UNC-CH NADIA Scientific Core

描述(由申请者提供)：这个NAIDA核心的主要目标是整合和扩大联盟的研究项目。科学核心的假设是，青春期间歇性乙醇(AIE)影响大脑发育，并诱导特定的神经适应，作为一种特定的神经化学和形态表型持续到成年。因此，科学核心将通过提供神经化学和形态脑图集来服务和连接NADIA组件，这些图集将定义青春期接触酒精的大脑的表型。每个组件将把大脑送到核心，用于免疫组织化学检测与被研究的生理和行为相关的大脑区域的关键神经递质基因的表达。此外，磁共振成像(MRI)和扩散张量成像(DTI)数据将在选定的数据集上收集，以提供不同AIE模型对成人大脑影响的全局结构信息。最后，所有结果数据都将被共享，未使用的脑组织将被存档，以便将来进行分析。总之，这些方法将提供有关ALE诱导的成人行为和生理变化背后的神经生物学机制的关键信息。科学核心中心将使用青春期饮酒的动物模型来研究酒精如何改变大脑结构和神经化学。这一努力，以及由此产生的数据存储库，有助于整合NAIDA联合会的研究组成部分，并将为了解青少年生活轨迹的差异和重要行为模式的成熟提供基础。

DESCRIPTION (provided by applicant): The primary objective of this NADIA core is to integrate and expand the Consortium research projects. The hypothesis of the Scientific Core is that adolescent intermittent ethanol (AIE) impacts brain development and induces specific neuro-adaptations that persist into adulthood as a specific neurochemical and morphological phenotype. Thus, the Scientific Core will serve and link NADIA components by providing neurochemical and morphological brain atlases that will define the phenotype of the brain exposed to alcohol during adolescence. Each component will send brains to the core for immunohistochemical determination of pivotal neurotransmitter gene expression in brain regions related to the physiology and behaviors being studied. Additionally, Magnetic Resonance Imaging (MRI) and Diffusion Tensor Imaging (DTI) data will be collected on select data sets in order to provide global structural information on the impact of different AIE models on adult brain. Finally, all the resulting data will be shared and unused brain tissue will be archived to allow for future analysis. Together, these approaches will provide critical information on neurobiological mechanisms that underlie AlE-induced changes in adult behavior and physiology. The Scientific Core will use an animal model of adolescence alcohol drinking to investigate how alcohol changes brain structure and neurochemistry. This endeavor, along with the resulting data repository, serves to integrate the research components of the NADIA Consortium and will provide a foundation for understanding differences in adolescent life trajectories and maturation of important behavioral repertoires.