UNC-CH NADIA Scientific Core

描述（由申请人提供）：该NADIA核心的主要目标是整合和扩展联盟的研究项目。科学核心的假设是，青少年间歇性乙醇（AIE）影响大脑发育，并诱导特定的神经适应，作为特定的神经化学和形态表型持续到成年期。因此，科学核心将通过提供神经化学和形态学大脑图谱来服务和链接NADIA组件，这些图谱将定义青春期暴露于酒精的大脑的表型。每个组件将发送大脑的核心免疫组织化学测定关键神经递质基因表达的大脑区域相关的生理和行为正在研究。此外，将收集选定数据集的磁共振成像（MRI）和扩散张量成像（DTI）数据，以提供不同AIE模型对成人大脑影响的总体结构信息。最后，所有产生的数据将被共享，未使用的脑组织将被存档，以供未来分析。总之，这些方法将提供关键信息的神经生物学机制的基础铝诱导成人的行为和生理变化。科学核心将使用青少年饮酒的动物模型来研究酒精如何改变大脑结构和神经化学。这一奋进，沿着由此产生的数据库，有助于整合NADIA联盟的研究组成部分，并将为理解青少年生活轨迹的差异和重要行为剧目的成熟提供基础。

DESCRIPTION (provided by applicant): The primary objective of this NADIA core is to integrate and expand the Consortium research projects. The hypothesis of the Scientific Core is that adolescent intermittent ethanol (AIE) impacts brain development and induces specific neuro-adaptations that persist into adulthood as a specific neurochemical and morphological phenotype. Thus, the Scientific Core will serve and link NADIA components by providing neurochemical and morphological brain atlases that will define the phenotype of the brain exposed to alcohol during adolescence. Each component will send brains to the core for immunohistochemical determination of pivotal neurotransmitter gene expression in brain regions related to the physiology and behaviors being studied. Additionally, Magnetic Resonance Imaging (MRI) and Diffusion Tensor Imaging (DTI) data will be collected on select data sets in order to provide global structural information on the impact of different AIE models on adult brain. Finally, all the resulting data will be shared and unused brain tissue will be archived to allow for future analysis. Together, these approaches will provide critical information on neurobiological mechanisms that underlie AlE-induced changes in adult behavior and physiology. The Scientific Core will use an animal model of adolescence alcohol drinking to investigate how alcohol changes brain structure and neurochemistry. This endeavor, along with the resulting data repository, serves to integrate the research components of the NADIA Consortium and will provide a foundation for understanding differences in adolescent life trajectories and maturation of important behavioral repertoires.