MEASUREMENT OF CEREBRAL OXIDATIVE METABOLISM WITH SIMULTANEOUS MRI AND DOS

MRI 和 DOS 同步测量脑氧化代谢

• 批准号: 8362015
• 负责人: David Pilkinton
• 金额: $ 0.77万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362015
• 关键词: 2,4-Dinitrophenol; Animal Model; Blood; Brain; Brain Drains; Breathing; Cell Respiration; Cerebrum; Diffuse; Family suidae; Funding; Gases; Goals; Grant; Human; Magnetic Resonance; Magnetic Resonance Imaging; Measurement; Measures; Metabolic; Metabolism; Methods; National Center for Research Resources; Optics; Oxygen; Phase; Principal Investigator; Relative (related person); Research; Research Infrastructure; Resources; Sagittal Sinus; Source; Spectrum Analysis; Systematic Bias; Techniques; United States National Institutes of Health; Venous; Weight; cost; lung volume; metabolic poison; optical imaging; research study

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The goal of this project is to simulatneously measure the cerebral metabolic rate of oxygen (CMRO2) with MRI and diffuse optical spectroscopy (DOS). Multimodal MRI measurements will be made, including (1) local relative CMRO2 using a BOLD/ASL T¿2-weighted fast spin echo sequence calibrated using the inhalation of separate hyperoxic and hypercapnic epochs, (2) absolute whole brain CMRO2¿ by measuring the phase and/or T2 and the velocity using phase contrast measurements of venous blood draining from the brain into the sagittal sinus, and (3) local absolute CMRO¿2 using inhaled oxygen-17 (17O) gas. DOS will be used to simultaneouly measure whole brain relaxtive CMRO2. Swine -- a large animal model with similar size and lung volume to that of humans -- will be used in lieu of human experiments for a two major reasons. First, 17O is not yet approved for human use. Second, we will produce a large increase in metabolism in the brain by using the metabolic poison 2,4-dinitrophenol (DNP), which cannot be used in humans. Our goal is to cross-validate the 17O and DOS measurements with more established methods of local and whole brain CMRO2 MRI measurement techniques, and determine whether the techniques produce different estimates and/or have sources of systematic bias.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 本研究旨在利用磁共振成像（MRI）和扩散光谱（DOS）技术同时测量脑氧代谢率（CMRO 2）。 将进行多模式MRI测量，包括（1）使用BOLD/ASL T2加权快速自旋回波序列进行局部相对CMRO 2，该序列使用单独的高氧和高碳酸时期的吸入进行校准，（2）通过使用从大脑流入矢状窦的静脉血的相位对比测量测量相位和/或T2和速度来测量绝对全脑CMRO 2，以及（3）局部绝对CMRO 2。2使用吸入的氧-17（17 O）气体。 DOS将被用于间接测量全脑弛豫CMRO 2。 猪是一种大型动物模型，其大小和肺体积与人类相似，将用于代替人类实验，主要有两个原因。 第一，17 O尚未被批准用于人类。 第二，我们将通过使用不能用于人类的代谢毒物2，4-二硝基苯酚（DNP）来大量增加大脑的代谢。 我们的目标是交叉验证17 O和DOS测量与更成熟的方法，局部和全脑CMRO 2 MRI测量技术，并确定是否技术产生不同的估计和/或有系统性偏差的来源。