SPINAL CORD ASTROCYTES AND CHRONIC PAIN

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 我们正在研究慢性疼痛对脊髓星形胶质细胞的影响。为了补充对脊髓星形胶质细胞的电生理测量，我们想在光镜和电子显微镜水平上观察星形胶质细胞的形态变化。我们希望通过结合荧光染料细胞填充、光转换和电子断层扫描来实现这一点。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. We are studying the consequences of chronic pain on astrocytes in the spinal cord. In order to complement electrophysiological measurements being made on spinal astrocytes, we would like to examine changes in the morphology of astrocytes at the light and electron microscopic levels. We would like to approach this with a combination of fluorescent dye cell fills, photoconversion, and electron tomography.