AIR-CONTAINING LIPOSOMES

含气脂质体

• 批准号: 8361127
• 负责人: David D McPherson
• 金额: $ 0.49万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361127
• 关键词: Acoustics; Air; Contrast Media; Drug Formulations; Electron Microscopy; Encapsulated; Freeze Fracturing; Funding; Gases; Genes; Grant; Image; Image Enhancement; Lipid Bilayers; Liposomes; Mannitol; National Center for Research Resources; Pharmaceutical Preparations; Phase; Population; Preparation; Principal Investigator; Research; Research Infrastructure; Resources; Sampling; Solutions; Source; Structure; Thermodynamics; Ultrasonography; United States National Institutes of Health; aqueous; cost; falls; macromolecule; monolayer

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Liposomes are self-forming lipid bilayer arrays separating an aqueous internal compartment from the bulk aqueous phase with clearly separated hydrophilic and hydrophobic regions. Initially, liposomal preparations were modified to permit gas encapsulation in order to be used as a targetable contrast agent for ultrasound imaging enhancement. These preparations have been termed "echogenic liposomes (ELIP)". When a gas is encapsulated into a liposome, the gas can be presumed, for thermodynamic reasons, to reside between the two monolayers of the liposome bilayer or as a monolayer covered air bubble within the aqueous compartment of liposomes. Our samples are liposomes in 0.32 M mannitol solution. The size distribution of of the liposomes falls into two primary populations, 100 nm and 1,000 nm, with the average size at 800nm. In our previous research, we have successfully co-encapsulated drugs and genes into ELIP and have imaged our ELIP using freeze fracture electron microscopy. The highly acoustic liposomes have demonstrated a spherical structure with the acoustic formulation.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 脂质体是自形成的脂质双层阵列，其将水性内部隔室与主体水相分离，具有明显分离的亲水性和疏水性区域。最初，脂质体制剂 被修改以允许气体封装，以便用作超声成像增强的可靶向造影剂。这些制剂被称为"回声脂质体（ELIP）"。当气体 在包封到脂质体中的情况下，出于热力学原因，可以假定气体驻留在脂质体双层的两个单层之间，或者作为单层覆盖的气泡驻留在水性脂质体中。 脂质体的隔室。 我们的样品是0.32 M甘露醇溶液中的脂质体。 脂质体福尔斯的粒径分布分为100 nm和1,000 nm两个主要群体，平均粒径为800 nm。 在我们以前的研究中，我们已经成功地将药物和基因共封装到ELIP中，并使用冷冻断裂电子显微镜对我们的ELIP进行成像。高声学脂质体已经证明了 球形结构与声学公式。