DNA DOUBLE STRAND BREAK REPAIR

DNA 双链断裂修复

• 批准号: 8362690
• 负责人: Michael W. Berns
• 金额: $ 0.51万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362690
• 关键词: Ablation; Biotechnology; Cell Cycle Stage; Cells; Collaborations; DNA Damage; DNA Double Strand Break; DNA Repair; Double Strand Break Repair; Funding; Goals; Grant; Hela Cells; Image; Kinetics; Lasers; Lesion; Microscope; Mitosis; Mitotic; National Center for Research Resources; Pathway interactions; Principal Investigator; Research; Research Infrastructure; Resources; Source; Specificity; Staining method; Stains; United States National Institutes of Health; cost; irradiation; nanosecond; repaired; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Essentially this project concerns the use of laser ablation as a platform to study DNA double strand break repair during mitosis. In collaboration with the LAMMP, I will use the Meta 510 confocal microscope to perform flourescent imaging of HeLa and CFPAC1 cells stained for various DSB recognition and repair factors after lesion induction in a different facility with femtosecond near IR or nanosecond UVA laser irradiation. Goals include: elucidation of pathway specificity in the cellular response to mitotic DNA damage and the kinetics of DNA damage repair factor recruitment during the different stages of the cell cycle.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其他NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 本质上，该项目涉及使用激光消融作为研究有丝分裂期间DNA双链断裂修复的平台。 与LAMMP合作，我将使用Meta 510共聚焦显微镜对HeLa和CFPAC 1细胞进行荧光成像，这些细胞在不同的设施中用飞秒近红外或纳秒UVA激光照射进行损伤诱导后，针对各种DSB识别和修复因子进行染色。 目标包括：阐明了细胞对有丝分裂DNA损伤的反应途径特异性和细胞周期不同阶段DNA损伤修复因子募集的动力学。