DNA DOUBLE STRAND BREAK REPAIR

DNA 双链断裂修复

• 批准号: 8169519
• 负责人: Michael W. Berns
• 金额: $ 0.34万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8169519
• 关键词: Ablation; Cell Cycle Stage; Cells; Collaborations; Computer Retrieval of Information on Scientific Projects Database; DNA Damage; DNA Double Strand Break; DNA Repair; Double Strand Break Repair; Funding; Goals; Grant; Hela Cells; Image; Institution; Kinetics; Lasers; Lesion; Microscope; Mitosis; Mitotic; Pathway interactions; Research; Research Personnel; Resources; Source; Specificity; Staining method; Stains; United States National Institutes of Health; irradiation; nanosecond; repaired; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Essentially this project concerns the use of laser ablation as a platform to study DNA double strand break repair during mitosis. In collaboration with the LAMMP, I will use the Meta 510 confocal microscope to perform flourescent imaging of HeLa and CFPAC1 cells stained for various DSB recognition and repair factors after lesion induction in a different facility with femtosecond near IR or nanosecond UVA laser irradiation. Goals include: elucidation of pathway specificity in the cellular response to mitotic DNA damage and the kinetics of DNA damage repair factor recruitment during the different stages of the cell cycle.

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 本质上，这个项目涉及使用激光消融作为一个平台，研究DNA双链断裂修复在有丝分裂。 与LAMMP合作，我将使用Meta 510共聚焦显微镜对HeLa和CFPAC 1细胞进行荧光成像，这些细胞在不同的设施中用飞秒近红外或纳秒UVA激光照射进行损伤诱导后，针对各种DSB识别和修复因子进行染色。 目标包括：阐明了细胞对有丝分裂DNA损伤的反应途径特异性和细胞周期不同阶段DNA损伤修复因子募集的动力学。