INTEGRATED PLATFORM FOR CHEMICAL ANALYSIS OF LIVE CELLS

描述：（申请书中的逐字逐句）本提案的总体目标 设计、建造和测试一个集成的光学和微流体系统， 这将使新的生物化学测定的性能在单一的生活， 细胞该设备将在生物医学系统中进行测试，主要涉及 癌症，细胞生长和发育，尽管它将有广泛的应用 在分子医学和药物开发领域。这个项目是 提交响应PAS-99-010，以支持生物工程研究 伙伴关系（BRP）。它涉及三个学术机构之间的密切合作， 研究实验室，每个都有非常不同的重点和专业知识：（1） 光子学/显微镜（Berns & Venugopalan）;（2）BioMEMS/微流体（Li & Bachman）;（3）分析化学/细胞生物学（Albritton &西姆斯）。 该项目的具体目标是：（1）研制激光显微镜 单细胞操作和分析平台;（2）开发一种 用于单细胞测定的多用途、模块化微流体芯片;（3） 开发了可以在单细胞中测定的广泛的分析物。 显微镜平台的开发将涉及：（a）基础研究， 表征和优化激光与 细胞和BioMEMS材料，以及（B）开发荧光模块， 在集成微流体系统中检测底物和分析物。 微流体系统的开发将涉及（a）基础工程 MEMS微加工工艺，（B）不同芯片设计工程 配置，和（c）生物材料相容性研究。此外该 BioMEMS微流控系统将与显微镜 平台和下文所述的化学分析系统。第三特定 其目的是进一步完善一种独特的新生物测定系统， 开发用于激活激酶和蛋白酶的新酶测定法，以及 将这些测定转移到集成装置。一个多学科 方法是需要完成的任务，在发展和 这个系统的整合。技术目标的实现 整合到一个平台上，将提供一种具有广泛 在分子医学和生物医学研究中的各种应用。

DESCRIPTION: (Verbatim from the application) The overall aim of this proposal is to design, build, and test an integrated optical and microfluidics system that will enable the performance of novel biochemical assays in single living cells. The device will be tested in biomedical systems relating primarily to cancer, and cell growth, and development, though it will have wide application in the areas of molecular medicine and drug development. This project is submitted in response to the PAS-99-010 to support Bioengineering Research Partnerships (BRP's). It involves a close collaboration amongst three academic research labs, each with a very different focus and expertise: (1) photonics/microscopy (Berns & Venugopalan); (2) BioMEMS/microfluidics (Li & Bachman); (3) analytical chemistry/cell biology (Albritton & Sims). The project's specific aims are: (1) the development of a laser microscope platform for single cell manipulation and analysis; (2) development of a multipurpose, modular microfluidics chip for single cell assays; (3) development of a broad range of analytes which can be assayed in single cells. Development of the microscope platform will involve, (a) basic studies to characterize and optimize the physical mechanisms of laser interaction with the cells and BioMEMS materials, and (b) development of a fluorescence module for detection of substrates and analytes in the integrated microfluidic system. Development of the microfluidic system will involve (a) basic engineering of the MEMS microfabrication process, (b) design engineering of different chip configurations, and (c) biomaterials compatibility studies. In addition, the BioMEMS microfluidic systems will be integrated with both the microscope platform and the chemical analysis systems described below. The third specific aim involves further perfection of a unique new bioassay system through the development of new enzyme assays for activation of kinases and proteases, and the transfer of these assays to the integrated device. A multidisciplinary approach is required to accomplish the tasks involved in the development and integration of this system. The achievement of the technological objectives interfaced into one platform will provide an enabling technology with a wide variety of applications in molecular medicine and biomedical research.