INTEGRATED PLATFORM FOR CHEMICAL ANALYSIS OF LIVE CELLS

描述：(逐字摘自申请表)本提案的总体目标 是设计、建造和测试一个集成的光学和微流体系统 这将使新的生化分析在单一生物中的表现成为可能 细胞。该设备将在生物医学系统中进行测试，主要涉及 癌症，细胞的生长和发育，尽管它将有广泛的应用 在分子医学和药物开发领域。这个项目是 提交以响应PAS-99-010以支持生物工程研究 合伙企业(BRP)。它涉及三位学者之间的密切合作 研究实验室，每个实验室都有非常不同的侧重点和专业知识：(1) 光子学/显微镜(Berns&Venugopalan)；(2)生物微机械/微流体(Li& (3)分析化学/细胞生物学(Albritton&Sims)。 该项目的具体目标是：(1)研制激光显微镜 用于单细胞操作和分析的平台；(2)开发一个 用于单细胞分析的多用途、模块化微流控芯片； 开发可在单细胞中进行分析的多种分析物。 显微镜平台的开发将涉及：(A)基础研究 描述和优化激光与激光相互作用的物理机制 细胞和生物微机械材料，以及(B)开发用于 集成微流控系统中底物和分析物的检测。 微流控系统的开发将涉及(A)基础工程 MEMS微加工工艺，(B)不同芯片的设计工程 (C)生物材料兼容性研究。此外， BioMEMS微流控系统将与这两种显微镜集成 平台和下文所述的化学分析系统。第三个具体问题 AIM涉及通过以下方式进一步完善独特的新生物检测系统 开发新的激活激酶和蛋白水解酶的酶分析方法； 将这些化验结果转移到集成设备。一个多学科的 需要一种方法来完成开发所涉及的任务 该系统的集成。技术目标的实现 集成到一个平台中将提供广泛的支持技术 在分子医学和生物医学研究中的各种应用。

DESCRIPTION: (Verbatim from the application) The overall aim of this proposal is to design, build, and test an integrated optical and microfluidics system that will enable the performance of novel biochemical assays in single living cells. The device will be tested in biomedical systems relating primarily to cancer, and cell growth, and development, though it will have wide application in the areas of molecular medicine and drug development. This project is submitted in response to the PAS-99-010 to support Bioengineering Research Partnerships (BRP's). It involves a close collaboration amongst three academic research labs, each with a very different focus and expertise: (1) photonics/microscopy (Berns & Venugopalan); (2) BioMEMS/microfluidics (Li & Bachman); (3) analytical chemistry/cell biology (Albritton & Sims). The project's specific aims are: (1) the development of a laser microscope platform for single cell manipulation and analysis; (2) development of a multipurpose, modular microfluidics chip for single cell assays; (3) development of a broad range of analytes which can be assayed in single cells. Development of the microscope platform will involve, (a) basic studies to characterize and optimize the physical mechanisms of laser interaction with the cells and BioMEMS materials, and (b) development of a fluorescence module for detection of substrates and analytes in the integrated microfluidic system. Development of the microfluidic system will involve (a) basic engineering of the MEMS microfabrication process, (b) design engineering of different chip configurations, and (c) biomaterials compatibility studies. In addition, the BioMEMS microfluidic systems will be integrated with both the microscope platform and the chemical analysis systems described below. The third specific aim involves further perfection of a unique new bioassay system through the development of new enzyme assays for activation of kinases and proteases, and the transfer of these assays to the integrated device. A multidisciplinary approach is required to accomplish the tasks involved in the development and integration of this system. The achievement of the technological objectives interfaced into one platform will provide an enabling technology with a wide variety of applications in molecular medicine and biomedical research.