WILLIAM WEIS PRT TIME

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 该计划旨在确定参与细胞膜靶向，粘附和信号传导过程的蛋白质的结构，以建立它们相互作用的机制和特异性。 有三个主要领域正在调查中，所有这些都与晶体需要同步辐射。 1.免疫系统中的细胞表面碳水化合物识别，重点是血清甘露糖结合蛋白及其如何触发补体介导的细胞溶解，以及DC-SIGN，一种介导抗原呈递细胞激活T细胞的粘附分子，也用于捕获HIV并将其传递给T细胞。 2.细胞间连接的结构和装配，研究连接钙粘蛋白细胞粘附分子与细胞骨架的蛋白质的相互作用。 粘附连接蛋白β-连环蛋白也是Wnt信号通路的重要组成部分，其与其他Wnt通路组分的相互作用也正在研究中。 3.负责细胞内囊泡与靶膜特异性对接和融合的蛋白质类。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This program aims to determine the structures of proteins involved in cell membrane targeting, adhesion and signaling processes, in order to establish the mechanism and specificity of their interactions. There are three principal areas under investigation, all of which with crystals that require synchrotron radiation. 1. Cell-surface carbohydrate recognition in the immune system, focusing on serum Mannose-binding Proteins and how they trigger complement-mediated cell lysis, and DC-SIGN, an adhesion molecule that mediates activation of T cells by antigen-presenting cells, and which also serves to capture HIV and deliver it to T cells. 2. Architecture and assembly of intercellular junctions, studying the interactions of the proteins that link cadherin cell adhesion molecules to the cytoskeleton. The adherens junction protein beta-catenin is also an essential component of the Wnt signaling pathway, and its interactions with other Wnt pathway components are also being studied. 3. Proteins responsible for specific docking and fusion of intracellular vesicles with target membranes.