PILATUS3 X 1M X-ray detector
PILATUS3 X 1M X-ray detector
批准号:
9074860
负责人:
William I Weis
金额:
$55.01万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2016
资助国家:
美国
项目状态:
已结题
起止时间:
2016-04-01 至 2018-03-31
关键词:
BiologicalBiomedical ResearchCatalysisCommunitiesComplexCoupledCryoelectron MicroscopyCrystallographyDataFundingGrowthHealthHumanInternationalMeasurementMethodsMolecular Sieve ChromatographyMutationNoiseOpticsPhotonsProteinsReadingResearchResearch PersonnelResolutionRoentgen RaysSignal TransductionSiliconSourceStructureSystemTechnologyTimeUnited States National Institutes of Healthdata acquisitiondetectordrug developmentimprovedmacromolecular assemblymacromoleculeprotein protein interactionresearch studystructural biologysynchrotron radiationtime usetool
中文摘要
英文摘要
DESCRIPTION (provided by applicant): Biological small angle x-ray scattering and diffraction (SAXS) provides structural and dynamic information from solutions or partially ordered arrays of biomolecules in physiologically relevant conditions, albeit at lower resolution (~7-10 Å or higher compared to that obtainable from crystallography or cryo-electron microscopy. SAXS is not only a powerful tool to study structures of large macromolecular assemblies in solution, but can also be used for time-resolved studies to investigate large conformational changes that occur during enzymatic catalysis, protein- protein interactions, and folding of macromolecules. Such studies are critical for biomedical research, as they provide basic mechanistic information that can be used to understand the effects of deleterious mutations, and can thereby inform drug development that ultimately impacts human health. The application of SAXS methods to problems in structural biology has seen tremendous growth in recent years, reflecting the need within the research community for experimental methods that can give structural and dynamic information on large multi-domain complexes. The explosive growth of SAXS has largely been enabled by technical advances that include high- brightness, low divergence X-ray sources, advances in X-ray optics, and X-ray detectors. As protein solutions scatter X-rays quite weakly, improved X-ray detectors are a critical enabling technology. The silicon pixel array X-ray detectors are ideally suited for SAXS, due to their zero read noise and photon counting technology, which open up new possibilities for SAXS applications in structural biology. This application is a request for a complete detector system for small angle X-ray scattering, consisting of a PILATUS3 X 1M Pixel Array Detector (PAD) manufactured by Dectris, Inc., and associated control, data acquisition, data handling and storage computing hardware. This system will be installed on beam line 4-2 (BL4-2) at the Stanford Synchrotron Radiation Lightsource (SSRL). The PILATUS3 1M PAD offers many advantages over the presently available detectors installed at the beam line including superior signal-to-noise, increased maximum frame rate, and larger dynamic range. The detector is well suited for size-exclusion chromatography coupled SAXS experiments, time-resolved, and high-throughput static SAXS measurements that will be needed to drive the forefront of BL4-2 research in structural biology on challenging biomedically important systems. It will maintain international competitiveness for NIH funded researchers.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Nanobody- and mini-G protein-enabled molecular pharmacology of HCAR1
-
批准号:10666999
-
项目类别:
-
资助金额:$15.46万
-
财政年份:2023
-
负责人:William I Weis
-
依托单位:
Molecular mechanisms of Wnt and mechanical signaling through β-catenin
-
批准号:10404076
-
项目类别:
-
资助金额:$73.31万
-
财政年份:2019
-
负责人:William I Weis
-
依托单位:
Molecular mechanisms of Wnt and mechanical signaling through β-catenin
-
批准号:10299581
-
项目类别:
-
资助金额:$18.83万
-
财政年份:2019
-
负责人:William I Weis
-
依托单位:
Molecular mechanisms of Wnt and mechanical signaling through β-catenin
-
批准号:10382116
-
项目类别:
-
资助金额:$2.21万
-
财政年份:2019
-
负责人:William I Weis
-
依托单位:
Molecular Basis of Wnt Receptor Interactions
-
批准号:8441547
-
项目类别:
-
资助金额:$30.61万
-
财政年份:2011
-
负责人:William I Weis
-
依托单位:
Molecular Mechanisms of Wnt Signal Transduction
-
批准号:9245707
-
项目类别:
-
资助金额:$31.09万
-
财政年份:2011
-
负责人:William I Weis
-
依托单位:
WILLIAM WEIS PRT TIME
-
批准号:8362039
-
项目类别:
-
资助金额:$0.74万
-
财政年份:2011
-
负责人:William I Weis
-
依托单位:
CELL SURFACE SIGNALING MOLECULES
-
批准号:8362414
-
项目类别:
-
资助金额:$0.38万
-
财政年份:2011
-
负责人:William I Weis
-
依托单位:
Molecular Mechanisms of Wnt Signal Transduction
-
批准号:9269721
-
项目类别:
-
资助金额:$7.98万
-
财政年份:2011
-
负责人:William I Weis
-
依托单位:
STRUCTURAL BASIS OF CELL MEMBRANE TARGETING, ADHESION, AND SIGNALING
-
批准号:8362199
-
项目类别:
-
资助金额:$0.06万
-
财政年份:2011
-
负责人:William I Weis
-
依托单位:
Molecular Basis of Wnt Receptor Interactions
-
批准号:8080131
-
项目类别:
-
资助金额:$32.13万
-
财政年份:2011
-
负责人:William I Weis
-
依托单位:
Molecular Basis of Wnt Receptor Interactions
-
批准号:8244425
-
项目类别:
-
资助金额:$32.39万
-
财政年份:2011
-
负责人:William I Weis
-
依托单位:
STRUCTURAL BASIS OF CELL MEMBRANE TARGETING, ADHESION, AND SIGNALING
-
批准号:8362111
-
项目类别:
-
资助金额:$0.03万
-
财政年份:2011
-
负责人:William I Weis
-
依托单位:
DETERMINING THE MOLECULAR ENVELOPE OF THE COMPLEX OF A G-PROTEIN AND ITS RECEPTO
-
批准号:8362362
-
项目类别:
-
资助金额:$0.74万
-
财政年份:2011
-
负责人:William I Weis
-
依托单位:
Molecular Basis of Wnt Receptor Interactions
-
批准号:8665349
-
项目类别:
-
资助金额:$32.39万
-
财政年份:2011
-
负责人:William I Weis
-
依托单位:
Molecular Mechanisms of Wnt Signal Transduction
-
批准号:9106024
-
项目类别:
-
资助金额:$31.04万
-
财政年份:2011
-
负责人:William I Weis
-
依托单位:
STRUCTURAL BASIS OF CELL MEMBRANE TARGETING, ADHESION, AND SIGNALING
-
批准号:8170160
-
项目类别:
-
资助金额:$0.03万
-
财政年份:2010
-
负责人:William I Weis
-
依托单位:
A Large-area Pixel Array Detector System for Macromolecular Crystallography
-
批准号:7822519
-
项目类别:
-
资助金额:$207.93万
-
财政年份:2010
-
负责人:William I Weis
-
依托单位:
Catenins, cadherins and their interactions
-
批准号:8000022
-
项目类别:
-
资助金额:$8.25万
-
财政年份:2010
-
负责人:William I Weis
-
依托单位:
DETERMINING THE MOLECULAR ENVELOPE OF THE COMPLEX OF A G-PROTEIN AND ITS RECEPTO
-
批准号:8170237
-
项目类别:
-
资助金额:$0.17万
-
财政年份:2010
-
负责人:William I Weis
-
依托单位:
海外基金