CELL SURFACE SIGNALING MOLECULES

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。次级项目的主要支助 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 表示子项目使用的中心基础设施的估计数量， NCRR赠款不直接向子项目或子项目工作人员提供资金。 该计划旨在确定参与两种不同类型的细胞信号级联的蛋白质的结构，以建立它们相互作用的机制和特异性：Wnt生长因子信号传导，其指定胚胎发生期间的细胞命运，成人组织更新，并在许多癌症中改变;和G蛋白偶联受体信号传导，特别是β 2-肾上腺素能受体，其响应于儿茶酚胺配体并在心血管和肺生理学中具有重要作用。目前有两个项目的晶体需要同步辐射。1. Wnt共受体LRP 6与活化和抑制性结合蛋白伴侣的相互作用。2.β 2-肾上腺素能受体的激活，检查其与激活小分子结合的结构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This program aims to determine the structures of proteins involved in two distinct kinds of cellular signaling cascades in order to establish the mechanism and specificity of their interactions: Wnt growth factor signaling, which specifies cell fate during embryogenesis, tissue renewal in the adult, and is altered in many cancers; and G-protein coupled receptor signaling, specifically the beta2-adrenergic receptor, which responds to catecholamine ligands and has important roles in cardiovascular and pulmonary physiology. There are currently two projects with crystals that require synchrotron radiation. 1. Interactions of the Wnt co-receptor LRP6 with activating and inhibitory binding protein partners. 2. Activation of the beta2-adrenergic receptor, examining its structure bound to activating small-molecule.