CELL SURFACE SIGNALING MOLECULES

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 该计划旨在确定两种不同类型的细胞信号级联反应中涉及的蛋白质的结构，以建立它们相互作用的机制和特异性：WNT生长因子信号，它决定了胚胎发育、成人组织更新过程中的细胞命运，并在许多癌症中发生变化；G蛋白偶联受体信号，特别是β2-肾上腺素能受体，它与儿茶酚胺配体反应，在心血管和肺生理学中具有重要作用。目前有两个项目的晶体需要同步辐射。1.Wnt共受体LRP6与激活和抑制结合蛋白对的相互作用2.激活β_2-肾上腺素能受体，检测其与激活小分子结合的结构。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This program aims to determine the structures of proteins involved in two distinct kinds of cellular signaling cascades in order to establish the mechanism and specificity of their interactions: Wnt growth factor signaling, which specifies cell fate during embryogenesis, tissue renewal in the adult, and is altered in many cancers; and G-protein coupled receptor signaling, specifically the beta2-adrenergic receptor, which responds to catecholamine ligands and has important roles in cardiovascular and pulmonary physiology. There are currently two projects with crystals that require synchrotron radiation. 1. Interactions of the Wnt co-receptor LRP6 with activating and inhibitory binding protein partners. 2. Activation of the beta2-adrenergic receptor, examining its structure bound to activating small-molecule.