PROTEOMIC STUDY OF BRASSINOSTEROID SIGNAL TRANSDUCTION
油菜素甾醇信号转导的蛋白质组学研究
基本信息
- 批准号:8363740
- 负责人:
- 金额:$ 1.44万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2011
- 资助国家:美国
- 起止时间:2011-06-01 至 2012-05-31
- 项目状态:已结题
- 来源:
- 关键词:Affinity ChromatographyBiochemicalCell Surface ReceptorsDataFundingGene ExpressionGeneticGoalsGrantGrowthLinkMass Spectrum AnalysisMolecularNational Center for Research ResourcesNuclearPathway interactionsPhosphoric Monoester HydrolasesPhosphorylationPhosphorylation SitePhosphotransferasesPlantsPrincipal InvestigatorProtein DephosphorylationProteinsProteomicsResearchResearch InfrastructureResearch Project GrantsResourcesRoleSignal TransductionSourceSteroidsTransgenic OrganismsUnited States National Institutes of Healthcostresearch studyresponsesteroid hormonetranscription factor
项目摘要
This subproject is one of many research subprojects utilizing the resources
provided by a Center grant funded by NIH/NCRR. Primary support for the subproject
and the subproject's principal investigator may have been provided by other sources,
including other NIH sources. The Total Cost listed for the subproject likely
represents the estimated amount of Center infrastructure utilized by the subproject,
not direct funding provided by the NCRR grant to the subproject or subproject staff.
Brassinosteriods (BRs) are steroid hormones with important roles in plants. BR signal is perceived by the cell-surface receptor kinase BRI1, which initiates a cascade of protein phosphorylation leading to nuclear gene expression and cellular responses. Downstream BR signaling involves the cytoplasmic GSK3/SHAGGY-like kinase BIN2. BR inactivate BIN2, which phosphorylates the transcription factors BZR1 and BZR2/BES1. Our proteomic studies combined with genetic approaches have identified several missing links, including BSKs kinases that transduce signals from BRI1 kinases to downstream components. The goal of this study is to further understand BR signal transduction at the biochemical and proteomic levels. First,we will identify the BZR1-interacting proteins using tandem affinity purification and mass spectrometry analysis. Brassinosteroid promotes growth by inducing dephosphorylation of transcription factor BZR1, but the phosphatase(s) that desphosphorylate BZR1 still remain(s) elusive. Genetic and CHIP-CHIP data have suggested BZR1 might interact with different transcription factors to regulate gene expression. This study will greatly help to identify the phosphotase and to elucidate the networks of transcription factors. Second, we will identify phosphorylation sites of several key components of this pathway (BSK, BSUs, CDGs, BIN2). Third, in order to understand how BR signaling regulates BIN2 activity, we will purify BIN2-interacting proteins and identify them using MS analysis. The functions of the identified proteins will be studied using genetic and transgenic experiments. This research project will advance our understanding of the molecular mechanism for steroid responses in plants, which will have broad implications in our understanding of steroid actions in general.
这个子项目是利用这些资源的众多研究子项目之一
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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ZHIYONG WANG其他文献
ZHIYONG WANG的其他文献
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{{ truncateString('ZHIYONG WANG', 18)}}的其他基金
FUNCTIONAL STUDIES OF BRASSINOSTEROID-REGULATED PROTEINS
油菜素类固醇调节蛋白的功能研究
- 批准号:
8363848 - 财政年份:2011
- 资助金额:
$ 1.44万 - 项目类别:
PROTEOMIC STUDY OF BRASSINOSTEROID RESPONSES IN PLANTS
植物中油菜素类固醇反应的蛋白质组学研究
- 批准号:
8363849 - 财政年份:2011
- 资助金额:
$ 1.44万 - 项目类别:
PROTEOMIC STUDY OF PROTEIN MODIFICATIONS IN PLANTS
植物中蛋白质修饰的蛋白质组学研究
- 批准号:
8363847 - 财政年份:2011
- 资助金额:
$ 1.44万 - 项目类别:
Genetic Biochemical Studies of Plant Steroid Signaling
植物类固醇信号传导的遗传生化研究
- 批准号:
7993689 - 财政年份:2010
- 资助金额:
$ 1.44万 - 项目类别:
PROTEOMIC STUDY OF BRASSINOSTEROID SIGNAL TRANSDUCTION
油菜素甾醇信号转导的蛋白质组学研究
- 批准号:
8169732 - 财政年份:2010
- 资助金额:
$ 1.44万 - 项目类别:
PROTEOMIC STUDY OF BRASSINOSTEROID SIGNAL TRANSDUCTION
油菜素甾醇信号转导的蛋白质组学研究
- 批准号:
7957369 - 财政年份:2009
- 资助金额:
$ 1.44万 - 项目类别:
PROTEOMIC STUDY OF BRASSINOSTEROID SIGNAL TRANSDUCTION
油菜素甾醇信号转导的蛋白质组学研究
- 批准号:
7724170 - 财政年份:2008
- 资助金额:
$ 1.44万 - 项目类别:
PROTEOMIC STUDY OF BRASSINOSTEROID SIGNAL TRANSDUCTION
油菜素甾醇信号转导的蛋白质组学研究
- 批准号:
7601819 - 财政年份:2007
- 资助金额:
$ 1.44万 - 项目类别:
PROTEOMIC: BRASSINOSTEROID SIGNAL TRANSDUCTION
蛋白质组学:油菜素类固醇信号转导
- 批准号:
7369050 - 财政年份:2006
- 资助金额:
$ 1.44万 - 项目类别:
PROTEOMIC: BRASSINOSTEROID SIGNAL TRANSDUCTION
蛋白质组学:油菜素类固醇信号转导
- 批准号:
7180946 - 财政年份:2005
- 资助金额:
$ 1.44万 - 项目类别:
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