PROTEOMIC STUDY OF BRASSINOSTEROID RESPONSES IN PLANTS

植物中油菜素类固醇反应的蛋白质组学研究

• 批准号: 8363849
• 负责人: ZHIYONG WANG
• 金额: $ 0.37万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363849
• 关键词: Affinity Chromatography; Biochemical; Biomass; Funding; Goals; Grant; Mass Spectrum Analysis; Methods; Molecular; Molecular Genetics; National Center for Research Resources; Plants; Principal Investigator; Production; Proteins; Proteomics; Regulation; Research; Research Infrastructure; Resources; Source; United States National Institutes of Health; cost; plant growth/development; response; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Our long-term goal is to identify molecular tools for increasing biomass production by understanding the molecular and biochemical mechanisms of brassinosteroid (BR) regulation of plant growth and development. Our strategy to achieve this goal is to identify BR-regulated proteins using proteomic methods, and then study their functions using molecular genetic and biochemical approaches. The specific goals of this proposal include: (1) illustrate the functions of a number of BR-regulated proteins identified by proteomic analysis; (2) identify interacting proteins of the BR-regulated protein using affinity purification and mass spectrometry; (3) identify additional BR early response proteins using prefractionation followed by 2-D DIGE and iTRAQ mass spectrometry; (4) determine the functions of a number of the newly identified proteins.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 我们的长期目标是通过了解油菜素类固醇(BR)调节植物生长发育的分子和生化机制来确定提高生物量产量的分子工具。我们实现这一目标的策略是使用蛋白质组学方法鉴定BR调节的蛋白质，然后使用分子遗传学和生物化学方法研究它们的功能。这一建议的具体目标包括：(1)通过蛋白质组学分析确定一些受BR调节的蛋白质的功能；(2)利用亲和纯化和质谱学鉴定BR调节蛋白的相互作用蛋白质；(3)通过预分馏和2-D DGE和iTRAQ质谱学鉴定其他BR早期反应蛋白；(4)确定一些新发现的蛋白质的功能。