PROTEOMIC STUDY OF BRASSINOSTEROID SIGNAL TRANSDUCTION

油菜素甾醇信号转导的蛋白质组学研究

基本信息

  • 批准号:
    7724170
  • 负责人:
  • 金额:
    $ 1.29万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2008
  • 资助国家:
    美国
  • 起止时间:
    2008-06-01 至 2009-05-31
  • 项目状态:
    已结题

项目摘要

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Brassinosteriods (BRs) are steroid hormones with important roles in plants. BR signal is perceived by the cell-surface receptor kinase BRI1, which initiates a cascade of protein phosphorylation leading to nuclear gene expression and cellular responses. Downstream BR signaling involves the cytoplasmic GSK3/SHAGGY-like kinase BIN2. BIN2 phosphorylates the transcription factors BZR1 and BZR2/BES1. Phosphorylation of the transcription factors inhibits their DNA binding and nuclear localization and promotes their degradation by the proteasome. The goal of this study is to understand BR signal transduction at the biochemical and proteomic levels. First, we will identify the BIN2-phosphorylation sites of BZR1 using mass spectrometry (MS). The importance of some of the phosphorylation sites will be further tested by site-directed-mutagenesis and transgenic experiments. Second, in order to understand how BR signaling regulates BIN2 activity, we will purify BIN2-interacting proteins and identify them using MS analysis. Third, we are identifying BR-regulated proteins using two-dimensional difference gel electrophoresis (2-D DIGE) and iTRAQ mass spectrometry. We will identify BR-regulated proteins by performing proteomic analysis of total protein, plasma membrane, and phosphoprotein fractions. Forth, we plan to study BR regulated protein phosphorylation using immobilized metal affinity chromatography and the iTRAQ method. The functions of the identified proteins will be studied using genetic and transgenic experiments. This research project will advance our understanding of the molecular mechanism for steroid responses in plants, which will have broad implications in our understanding of steroid actions in general.
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项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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ZHIYONG WANG其他文献

ZHIYONG WANG的其他文献

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{{ truncateString('ZHIYONG WANG', 18)}}的其他基金

FUNCTIONAL STUDIES OF BRASSINOSTEROID-REGULATED PROTEINS
油菜素类固醇调节蛋白的功能研究
  • 批准号:
    8363848
  • 财政年份:
    2011
  • 资助金额:
    $ 1.29万
  • 项目类别:
PROTEOMIC STUDY OF BRASSINOSTEROID RESPONSES IN PLANTS
植物中油菜素类固醇反应的蛋白质组学研究
  • 批准号:
    8363849
  • 财政年份:
    2011
  • 资助金额:
    $ 1.29万
  • 项目类别:
PROTEOMIC STUDY OF PROTEIN MODIFICATIONS IN PLANTS
植物中蛋白质修饰的蛋白质组学研究
  • 批准号:
    8363847
  • 财政年份:
    2011
  • 资助金额:
    $ 1.29万
  • 项目类别:
PROTEOMIC STUDY OF BRASSINOSTEROID SIGNAL TRANSDUCTION
油菜素甾醇信号转导的蛋白质组学研究
  • 批准号:
    8363740
  • 财政年份:
    2011
  • 资助金额:
    $ 1.29万
  • 项目类别:
Genetic Biochemical Studies of Plant Steroid Signaling
植物类固醇信号传导的遗传生化研究
  • 批准号:
    7993689
  • 财政年份:
    2010
  • 资助金额:
    $ 1.29万
  • 项目类别:
PROTEOMIC STUDY OF BRASSINOSTEROID SIGNAL TRANSDUCTION
油菜素甾醇信号转导的蛋白质组学研究
  • 批准号:
    8169732
  • 财政年份:
    2010
  • 资助金额:
    $ 1.29万
  • 项目类别:
PROTEOMIC STUDY OF BRASSINOSTEROID SIGNAL TRANSDUCTION
油菜素甾醇信号转导的蛋白质组学研究
  • 批准号:
    7957369
  • 财政年份:
    2009
  • 资助金额:
    $ 1.29万
  • 项目类别:
PROTEOMIC STUDY OF BRASSINOSTEROID SIGNAL TRANSDUCTION
油菜素甾醇信号转导的蛋白质组学研究
  • 批准号:
    7601819
  • 财政年份:
    2007
  • 资助金额:
    $ 1.29万
  • 项目类别:
PROTEOMIC: BRASSINOSTEROID SIGNAL TRANSDUCTION
蛋白质组学:油菜素类固醇信号转导
  • 批准号:
    7369050
  • 财政年份:
    2006
  • 资助金额:
    $ 1.29万
  • 项目类别:
PROTEOMIC: BRASSINOSTEROID SIGNAL TRANSDUCTION
蛋白质组学:油菜素类固醇信号转导
  • 批准号:
    7180946
  • 财政年份:
    2005
  • 资助金额:
    $ 1.29万
  • 项目类别:

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