PROTEOMIC STUDY OF PROTEIN MODIFICATIONS IN PLANTS

植物中蛋白质修饰的蛋白质组学研究

• 批准号: 8363847
• 负责人: ZHIYONG WANG
• 金额: $ 0.46万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363847
• 关键词: Acetylation; Arabidopsis; Environment; Enzymes; Funding; Goals; Grant; Growth; Hormones; Mass Spectrum Analysis; Modification; National Center for Research Resources; Neurons; Peptides; Phosphorylation; Plants; Play; Post-Translational Protein Processing; Post-Translational Regulation; Principal Investigator; Process; Proteomics; Regulation; Research; Research Infrastructure; Resources; Role; Sampling; Signal Transduction; Source; System; United States National Institutes of Health; cell growth regulation; cost; glycosylation; mutant; response

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The goal of this project is to analyze protein modifications in plants using mass spectrometry. Posttranslational modifications, such as glycosylation, phosphorylation, and acetylation, play important roles in cellular regulation. Because plants are immobile and lack neuron systems, responses to the changing environment relies on cellular regulatory mechanisms. Therefore, plants are believed to have more sophisticate posttranslational regulation systems, and thus good system for proteomic study of cellular regulation mechanisms. In this project, posttranslationally modified peptides will be enriched from plant samples and analyzed by mass spectrometry. The study will focus on glycosylation (O-Gluc-NAc) and acetylation, which are known to be important for hormone signaling and growth regulation in plants. Arabidopsis mutants for enzymes catalyzing these modification processes will be used as references in the studies.

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