PROTEOMIC STUDY OF PROTEIN MODIFICATIONS IN PLANTS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 这个项目的目标是利用质谱学分析植物中的蛋白质修饰。翻译后修饰，如糖基化、磷酸化和乙酰化，在细胞调节中起着重要作用。由于植物是不能移动的，缺乏神经系统，对不断变化的环境的反应依赖于细胞调节机制。因此，植物被认为具有更复杂的翻译后调节系统，因此是研究细胞调节机制的蛋白质组学的良好系统。在这个项目中，翻译后修饰的多肽将从植物样本中浓缩并用质谱仪进行分析。这项研究将集中在糖基化(O-Gluc-NAC)和乙酰化，这两个已知在植物激素信号和生长调节中起重要作用。拟南芥中催化这些修饰过程的酶的突变体将在研究中用作参考。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The goal of this project is to analyze protein modifications in plants using mass spectrometry. Posttranslational modifications, such as glycosylation, phosphorylation, and acetylation, play important roles in cellular regulation. Because plants are immobile and lack neuron systems, responses to the changing environment relies on cellular regulatory mechanisms. Therefore, plants are believed to have more sophisticate posttranslational regulation systems, and thus good system for proteomic study of cellular regulation mechanisms. In this project, posttranslationally modified peptides will be enriched from plant samples and analyzed by mass spectrometry. The study will focus on glycosylation (O-Gluc-NAc) and acetylation, which are known to be important for hormone signaling and growth regulation in plants. Arabidopsis mutants for enzymes catalyzing these modification processes will be used as references in the studies.