FUNCTIONAL STUDIES OF BRASSINOSTEROID-REGULATED PROTEINS

油菜素类固醇调节蛋白的功能研究

• 批准号: 8363848
• 负责人: ZHIYONG WANG
• 金额: $ 0.13万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363848
• 关键词: Affinity Chromatography; Arabidopsis; Binding; Blood Vessels; Cell Surface Receptors; Cell division; Cell membrane; Cells; Developmental Process; Disease Resistance; Funding; Gene Expression; Genetic; Germination; Grant; Mass Spectrum Analysis; Molecular; Molecular Genetics; National Center for Research Resources; Nuclear; Phosphotransferases; Physiological Processes; Plants; Play; Principal Investigator; Productivity; Proteins; Proteomics; Research; Research Infrastructure; Resources; Role; Seeds; Signal Pathway; Signal Transduction; Source; Two-Dimensional Gel Electrophoresis; United States National Institutes of Health; cost; gene function; improved; receptor; steroid hormone; stress tolerance; success; tool; two-dimensional

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Brassinosteroids (BRs) are plant steroid hormones, which play important roles in a wide range of physiological and developmental processes, including cell expansion, cell division, vascular differentiation, seed germination, photomorphogenesis, stress tolerance and disease resistance. Molecular genetic studies have identified several BR signal transduction components of the BR signaling pathway, through which BRs bind the cell surface receptors to regulate nuclear gene expression. However, there are still gaps in the current BR signaling pathway. In particular, the substrates of the receptor kinases that transduce the BR signal from plasma membrane to cytoplasmic components remain unknown. Despite the success of genetic studies, many genes' function cannot be discovered by forward genetic approaches, largely due to genetic redundancy. Therefore, alternative approaches are required to significantly advance our understanding of the BR signaling pathway. Thus, we have performed proteomic studies and identified BR-regulated proteins in Arabidopsis using two-dimensional difference gel electrophoresis (2-D DIGE) followed by mass spectrometry. This study aims at characterizing plasma membrane-localized BR-responsive proteins, including the BR-signaling kinases (BSKs). Functional studies of these proteins have demonstrated their key role as BRI1 substrate in the BR signaling pathway. We plan to identify additional interacting proteins of BSKs using affinity purification followed by mass spectrometry analysis. Such analysis will advance our understanding of BR signal transduction and provide molecular tools for improving plant productivity.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 类甾醇（BRs）是一类植物甾体激素，在植物的细胞增殖、细胞分裂、维管分化、种子萌发、光形态建成、抗逆性和抗病性等生理和发育过程中发挥重要作用。分子遗传学研究已经确定了BR信号转导途径的几个BR信号转导组分，BR通过这些组分结合细胞表面受体来调节核基因表达。然而，目前的BR信号通路仍存在空白。特别是，受体激酶的底物的BR信号从质膜到细胞质成分仍然未知。尽管遗传学研究取得了成功，但许多基因的功能无法通过正向遗传学方法发现，这主要是由于遗传冗余。因此，需要替代方法来显著推进我们对BR信号通路的理解。因此，我们进行了蛋白质组学研究，并确定BR调节蛋白在拟南芥中使用二维差异凝胶电泳（2-D DIGE），然后通过质谱。本研究旨在表征质膜定位的BR反应蛋白，包括BR信号激酶（BSKs）。 这些蛋白的功能研究已经证明了它们在BR信号通路中作为BRI 1底物的关键作用。我们计划通过亲和纯化和质谱分析来鉴定BSK的其他相互作用蛋白。这些分析将促进我们对BR信号转导的理解，并为提高植物生产力提供分子工具。