CHARACTERIZATION OF THE EARLY APOPTOTIC MITOCHONDRIAL PROTEOME
早期凋亡线粒体蛋白质组的表征
基本信息
- 批准号:8363803
- 负责人:
- 金额:$ 0.01万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2011
- 资助国家:美国
- 起止时间:2011-06-01 至 2012-05-31
- 项目状态:已结题
- 来源:
- 关键词:AddressAffinityApoptosisApoptoticBioinformaticsBiologyCell DeathChemicalsElectrophoresisEvolutionFundingGoalsGrantHistocompatibility TestingLabelMass Spectrum AnalysisMitochondriaMitochondrial ProteinsModelingN-terminalNational Center for Research ResourcesNuclearOrganellesPathway interactionsPeptide Signal SequencesPeptidesPreparationPrincipal InvestigatorProteinsProteomeProteomicsRelative (related person)ResearchResearch InfrastructureResourcesRibosomesRoleSamplingSignal TransductionSourceUnited States National Institutes of HealthYeastsbasecostmitochondrial membranenovelprotein aminoacid sequencesubtiligase
项目摘要
This subproject is one of many research subprojects utilizing the resources
provided by a Center grant funded by NIH/NCRR. Primary support for the subproject
and the subproject's principal investigator may have been provided by other sources,
including other NIH sources. The Total Cost listed for the subproject likely
represents the estimated amount of Center infrastructure utilized by the subproject,
not direct funding provided by the NCRR grant to the subproject or subproject staff.
Several proteomic studies of mammalian and yeast mitochondria have identified a growing list of over 1000 proteins in the mitochondrial proteome. The overlap between various studies is limited to 60-80% and the relative abundance of these components varies with tissue type. The number of mitochondrial proteins has been estimated at 2000, based on an endosymbiont model for mitochondrial evolution (Gabaldon, 2004), thus the mitochondrial proteome is still incomplete. The vast majority of mitochondrial proteins are nuclear encoded and are expected to contain N-terminal peptide signals used for mitochondrial targeting. Interestingly, only one third of the proteins in the mammalian mitochondrial proteome are predicted to contain a signal peptide motif using bioinformatic analysis, while another third are easily annotated with known mitochondrial enzymatic functions. The remaining third of the sample are typically novel mitochondrial localized proteins of ambiguous function, and in some studies this number is greater than half of the total protein list. Conspicuously absent from many of these protein lists are the apoptosis regulating proteins, which associate with mitochondrial membranes in a highly regulated pathway for cell death.
This project will address 1) functional annotation of mitochondrial proteins using controlled biology and pharmacological induction of early apoptosis signaling and 2) analysis of N-terminal peptide for targeting of proteins to mitochondria. To accomplish these goals, we are investigating free flow electrophoresis (FFE) purification of intact mitochondria, reducing contamination by high abundance ribosome and nuclear components. We are also using a chemical biology enzymatic approach to label with subtiligase the free N-termini of mitochondrial proteins for affinity capture. The role of the Mass Spectrometry Facility in this project is to handle all aspects of the organelle preparation, peptide sequencing and bioinformatic analysis of N-terminal peptides.
这个子项目是利用这些资源的众多研究子项目之一
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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{{ truncateString('ALMA L BURLINGAME', 18)}}的其他基金
UTILIZATION OF QSTARXL MASS SPECTROMETER, LC SYSTEM & ASSOCIATED SOFTWARE
QSTARXL 质谱仪、LC 系统的使用
- 批准号:
8363759 - 财政年份:2011
- 资助金额:
$ 0.01万 - 项目类别:
STRUCTURAL ANALYSIS OF SCORPION VENOM VAEJOVIS MEXICANUS SMITHI
墨西哥蝎毒VAEJOVIS MEXICANUS SMITHI的结构分析
- 批准号:
8363774 - 财政年份:2011
- 资助金额:
$ 0.01万 - 项目类别:
OPTIMIZATION OF ETD TECHNIQUE ON THE LTQ ORBITRAP
LTQ Orbitrap 上 ETD 技术的优化
- 批准号:
8363798 - 财政年份:2011
- 资助金额:
$ 0.01万 - 项目类别:
VISUALIZATION OF QUANTITATIVE BIOLOGICAL MASS SPECTROMETRY DATA
定量生物质谱数据的可视化
- 批准号:
8363629 - 财政年份:2011
- 资助金额:
$ 0.01万 - 项目类别:
DECIPHERING THE PHOSPHOPROTEOME OF T LYMPHOCYTES
破译 T 淋巴细胞的磷酸蛋白质组
- 批准号:
8363775 - 财政年份:2011
- 资助金额:
$ 0.01万 - 项目类别:
UTILIZATION OF QSTAR MASS SPECTROMETERS, LC SYSTEM & ASSOCIATED SOFTWARE
QSTAR 质谱仪、LC 系统的使用
- 批准号:
8363742 - 财政年份:2011
- 资助金额:
$ 0.01万 - 项目类别:
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