Purchase of Q-Exactive Mass Spectrometer

购买Q-Exactive质谱仪

• 批准号: 8447398
• 负责人: ALMA L BURLINGAME
• 金额: $ 50.69万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-15 至 2014-06-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8447398
• 关键词: Algorithms; Bacillus (bacterium); Biological; California; Caspase; Cleaved cell; Complex; Detection; Fourier Transform; Granzyme; HIV; Ions; Patients; Peptides; Performance; Pharmaceutical Preparations; Phosphorylation; Post-Translational Protein Processing; Protein Dynamics; Proteins; Research Personnel; Resolution; Sampling; Scanning; Site; Structure; Time; Ubiquitination; Universities; base; cohort; improved; instrument; kinase inhibitor; mass spectrometer; pathogen; protein complex; research study

DESCRIPTION (provided by applicant): The proposed Q Exactive instrument from Thermo Scientific is a robust, high throughput, and high resolution mass spectrometer(1,2) based on high performance quadrupole mass selection and high resolution Orbitrap ion detection. It implements enhanced Fourier transform or eFT(3) algorithm for improved mass resolving power. The proposed Q Exactive instrument has several advantages over our current most powerful LTQ Orbitrap Velos instrument. The scan rate of HCD MSMS is nearly three times higher and the MSMS product ion detection efficiency is also higher. The combined effect allows the instrument to identify more peptides. As demonstrated in our comparison experiments, 2-3X more peptides are identified on the proposed instrument over those on the Velos Orbitrap. The Q Exactive mass spectrometer will help users identify and quantify post-translational modifications (PTM) from complex biological samples, determine protein complex structures, and study protein dynamics using strategically different approaches. A suite of University of California investigators will be major users and be helped in the identification and quantification of post-translational modifications (including site-specific phosphorylation and ubiquitination, fo example), proteolytically cleaved substrate networks (e.g., caspases, granzymes), host-pathogen protein interaction dynamics (HIV, T. bacillus, etc.) and drug inhibitors of kinases in patient cohorts.

描述（由申请人提供）：Thermo Scientific提出的Q Exactive仪器是一种基于高性能四极杆质量选择和高分辨率轨道离子检测的鲁棒、高通量和高分辨率质谱仪（1,2）。它实现了增强的傅里叶变换或eFT(3)算法，以提高质量分辨率。与我们目前最强大的LTQ Orbitrap Velos仪器相比，提出的Q Exactive仪器有几个优势。HCD MSMS的扫描率提高了近3倍，MSMS产物离子检测效率也更高。这种综合效应使仪器能够识别更多的多肽。在我们的比较实验中证明，在我们提出的仪器上比在Velos Orbitrap上多识别2-3倍的肽。qexactive质谱仪将帮助用户从复杂的生物样品中识别和量化翻译后修饰（PTM），确定蛋白质复合物结构，并使用不同的策略方法研究蛋白质动力学。加州大学的一组研究人员将是主要用户，并在识别和量化方面得到帮助