Purchase of Q-Exactive Mass Spectrometer

描述（由申请人提供）：Thermo Scientific的申报Q Exactive仪器是一种基于高性能四极杆质量选择和高分辨率Orbitrap离子检测的耐用、高通量和高分辨率质谱仪（1，2）。它实现了增强的傅里叶变换或eFT（3）算法，以提高质量分辨能力。与我们目前最强大的LTQ Orbitrap Velos仪器相比，拟议的Q Exactive仪器具有几个优势。HCD MSMS的扫描速率提高了近三倍，MSMS产物离子检测效率也更高。组合效应允许仪器识别更多肽。如我们的比较实验所示，在所提出的仪器上鉴定的肽比在Velos Orbitrap上鉴定的肽多2- 3倍。Q Exactive质谱仪将帮助用户识别和量化复杂生物样品中的翻译后修饰（PTM），确定蛋白质复合物结构，并使用策略性不同的方法研究蛋白质动力学。一组加州大学的调查人员将成为主要用户，并在识别和量化方面得到帮助 翻译后修饰（例如，包括位点特异性磷酸化和泛素化），蛋白水解切割的底物网络（例如，半胱天冬酶，颗粒酶），宿主-病原体蛋白相互作用动力学（HIV，T.杆菌等）和激酶的药物抑制剂。

DESCRIPTION (provided by applicant): The proposed Q Exactive instrument from Thermo Scientific is a robust, high throughput, and high resolution mass spectrometer(1,2) based on high performance quadrupole mass selection and high resolution Orbitrap ion detection. It implements enhanced Fourier transform or eFT(3) algorithm for improved mass resolving power. The proposed Q Exactive instrument has several advantages over our current most powerful LTQ Orbitrap Velos instrument. The scan rate of HCD MSMS is nearly three times higher and the MSMS product ion detection efficiency is also higher. The combined effect allows the instrument to identify more peptides. As demonstrated in our comparison experiments, 2-3X more peptides are identified on the proposed instrument over those on the Velos Orbitrap. The Q Exactive mass spectrometer will help users identify and quantify post-translational modifications (PTM) from complex biological samples, determine protein complex structures, and study protein dynamics using strategically different approaches. A suite of University of California investigators will be major users and be helped in the identification and quantification of post-translational modifications (including site-specific phosphorylation and ubiquitination, fo example), proteolytically cleaved substrate networks (e.g., caspases, granzymes), host-pathogen protein interaction dynamics (HIV, T. bacillus, etc.) and drug inhibitors of kinases in patient cohorts.