STRUCTURAL ANALYSIS OF SCORPION VENOM VAEJOVIS MEXICANUS SMITHI

墨西哥蝎毒VAEJOVIS MEXICANUS SMITHI的结构分析

• 批准号: 8363774
• 负责人: ALMA L BURLINGAME
• 金额: $ 0.01万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363774
• 关键词: Amino Acid Sequence; Biochemical; Biogenic Amines; Biological; Cellular Membrane; Complex Mixtures; Enzymes; Epilepsy; Funding; Grant; High Pressure Liquid Chromatography; Human; Insecticides; Investigation; Knowledge; Label; Lipids; MALDI-TOF Mass Spectrometry; Mass Spectrum Analysis; Medical; Mexico; N-terminal; National Center for Research Resources; Nervous system structure; Nucleotides; Peptides; Phospholipase; Principal Investigator; Reporting; Research; Research Infrastructure; Resources; SHFM1 gene; Scorpion Venoms; Scorpions; Source; Toxic effect; Toxin; United States National Institutes of Health; Venoms; antimicrobial; cost; interest; liquid chromatography mass spectrometry; molecular mass; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The investigation of venoms is focused on the knowledge of the components directly related to the toxic effects for humans, but many components directly related to the toxic effects for humans, but many components have a broad spectrum of biological activities. This, in addition to the medical reasons, the study of the composition of the venom has a great interest for the discovery of new biochemical tools for the analysis of the operational mechanisms of the nervous system and cellular membranes. It has been reported that scorpion venom contains a variety of biologically active components: enzymes, peptides, nucleotides, lipids, biogenic amines and other unknown substances. Scorpion venoms are rich sources of peptides with a variety of pharmacological functions. Known pharmacological activities include antimicrobial, anti-epilepsy, insecticide, phospholipase and toxins. The scorpion Vaejovis mexicanus smithi is native from the state of Morelos, Mexico. This species has not been studied in depth previously. Preliminary studies have shown a complex mixture of components present in its venom, which makes it an interesting target of study for the search of new bioactive components. Crude venom was fractionated by RP-HPLC, and around 85 fractions were collected. Each fraction was subjected to MALDI-TOF mass spectrometry. The approximate size of different cannel-blocking peptides has been previously reported. Fractions containing components of such molecular masses were selected for further studies. Due to the complexity of the mixture and the size of the peptides of interest, Edman sequencing as well as LC/MS, CID and ECD could be utilized in order to decipher the amino acid sequence of the toxin components. A combination ECD analysis and selective N-terminal labeling can aid the de novo sequencing of large peptides.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 对蛇毒的研究主要集中在对与人体毒性作用直接相关的成分的了解上，但许多成分对人体的毒性作用直接相关，但许多成分具有广泛的生物活性。因此，除了医学上的原因外，对毒液成分的研究对于发现新的生化工具来分析神经系统和细胞膜的作用机制具有极大的兴趣。据报道，蝎毒中含有多种生物活性成分：酶、肽、核苷酸、脂类、生物胺等未知物质。蝎子毒液含有丰富的多肽，具有多种药理作用。已知的药理活性包括抗微生物、抗癫痫、杀虫剂、磷脂酶和毒素。墨西哥毒蝎Vaejovis micianus smithi原产于墨西哥莫雷洛斯州。本种以前没有被深入研究过。初步研究表明，其毒液中存在复杂的成分混合物，这使其成为寻找新的生物活性成分的有趣研究目标。用反相高效液相色谱法对粗毒进行分级，共收集到约85个级分。对每一组分进行MALDI-TOF质谱分析。不同的通道阻滞肽的大小大致相同，此前已有报道。选择含有这种分子质量组分的馏分进行进一步研究。由于混合物的复杂性和感兴趣的多肽的大小，可以利用Edman测序以及LC/MS、CID和ECD来破译毒素组分的氨基酸序列。结合ECD分析和选择性N-末端标记可以辅助大肽的从头测序。