STRUCTURAL ANALYSIS OF SCORPION VENOM VAEJOVIS MEXICANUS SMITHI

墨西哥蝎毒VAEJOVIS MEXICANUS SMITHI的结构分析

• 批准号: 8363774
• 负责人: ALMA L BURLINGAME
• 金额: $ 0.01万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363774
• 关键词: Amino Acid Sequence; Biochemical; Biogenic Amines; Biological; Cellular Membrane; Complex Mixtures; Enzymes; Epilepsy; Funding; Grant; High Pressure Liquid Chromatography; Human; Insecticides; Investigation; Knowledge; Label; Lipids; MALDI-TOF Mass Spectrometry; Mass Spectrum Analysis; Medical; Mexico; N-terminal; National Center for Research Resources; Nervous system structure; Nucleotides; Peptides; Phospholipase; Principal Investigator; Reporting; Research; Research Infrastructure; Resources; SHFM1 gene; Scorpion Venoms; Scorpions; Source; Toxic effect; Toxin; United States National Institutes of Health; Venoms; antimicrobial; cost; interest; liquid chromatography mass spectrometry; molecular mass; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The investigation of venoms is focused on the knowledge of the components directly related to the toxic effects for humans, but many components directly related to the toxic effects for humans, but many components have a broad spectrum of biological activities. This, in addition to the medical reasons, the study of the composition of the venom has a great interest for the discovery of new biochemical tools for the analysis of the operational mechanisms of the nervous system and cellular membranes. It has been reported that scorpion venom contains a variety of biologically active components: enzymes, peptides, nucleotides, lipids, biogenic amines and other unknown substances. Scorpion venoms are rich sources of peptides with a variety of pharmacological functions. Known pharmacological activities include antimicrobial, anti-epilepsy, insecticide, phospholipase and toxins. The scorpion Vaejovis mexicanus smithi is native from the state of Morelos, Mexico. This species has not been studied in depth previously. Preliminary studies have shown a complex mixture of components present in its venom, which makes it an interesting target of study for the search of new bioactive components. Crude venom was fractionated by RP-HPLC, and around 85 fractions were collected. Each fraction was subjected to MALDI-TOF mass spectrometry. The approximate size of different cannel-blocking peptides has been previously reported. Fractions containing components of such molecular masses were selected for further studies. Due to the complexity of the mixture and the size of the peptides of interest, Edman sequencing as well as LC/MS, CID and ECD could be utilized in order to decipher the amino acid sequence of the toxin components. A combination ECD analysis and selective N-terminal labeling can aid the de novo sequencing of large peptides.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 对毒液的研究重点是对与对人类的毒性作用直接相关的成分的了解，但许多成分与对人体的毒性作用直接相关，但许多成分具有广谱的生物活性。 这除了医学原因外，毒液成分的研究对于发现用于分析神经系统和细胞膜的运行机制的新生化工具具有很大的兴趣。 据报道，蝎毒中含有多种生物活性成分：酶、肽、核苷酸、脂类、生物胺和其他未知物质。 蝎毒是具有多种药理功能的肽的丰富来源。 已知的药理活性包括抗菌、抗癫痫、杀虫、磷脂酶和毒素。 Vaejovis mexicanus smithi 蝎子原产于墨西哥莫雷洛斯州。 此前尚未对该物种进行过深入研究。 初步研究表明其毒液中存在复杂的成分混合物，这使其成为寻找新生物活性成分的有趣研究目标。 通过 RP-HPLC 对粗毒液进行分级，收集了大约 85 个级分。 对每个级分进行 MALDI-TOF 质谱分析。 先前已报道过不同通道阻断肽的大致大小。 选择含有此类分子质量的组分的级分用于进一步研究。 由于混合物的复杂性和目标肽的大小，可以利用 Edman 测序以及 LC/MS、CID 和 ECD 来破译毒素成分的氨基酸序列。 ECD 分析和选择性 N 末端标记相结合有助于大肽的从头测序。