OPTIMIZATION OF ETD TECHNIQUE ON THE LTQ ORBITRAP

LTQ Orbitrap 上 ETD 技术的优化

• 批准号: 8363798
• 负责人: ALMA L BURLINGAME
• 金额: $ 1.1万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363798
• 关键词: Amino Acids; Detection; Development; Dissociation; Electron Transport; Funding; Grant; Ions; Mass Spectrum Analysis; National Center for Research Resources; Peptides; Post-Translational Protein Processing; Principal Investigator; Proteins; Research; Research Infrastructure; Resources; Side; Source; Techniques; United States National Institutes of Health; cost

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Electron transfer dissociation (ETD) can be used to provide unique and complementary fragment ions when compared to other dissociation techniques, such as collision induced dissociation (CID). Weak side chain bonds such as are observed at phosphorylated amino acids in peptides are often lost during CID, but preserved during ETD. This makes the technique useful for the detection and analysis of posttranslational modifications in peptides and intact proteins. This project is devoted to the development and improvement of ETD for such applications.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 与碰撞诱导解离(CID)等其他解离技术相比，电子转移解离(ETD)可用于提供独特和互补的碎片离子。弱侧链键，如在多肽中观察到的磷酸化氨基酸，在CID过程中经常丢失，但在ETD过程中保留。这使得该技术对于检测和分析多肽和完整蛋白质的翻译后修饰是有用的。本项目致力于为此类应用开发和改进ETD。