IDENTIFYING BIOMINERALIZATION EVENTS IN HEALTHY AND DISEASED TISSUES: BONE, DENT

识别健康和患病组织中的生物矿化事件：骨骼、凹痕

• 批准号: 8362403
• 负责人: Sunita P Ho
• 金额: $ 0.03万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362403
• 关键词: Apatites; Bone Tissue; Cell Nucleus; Collagen; Collagen Fiber; Collagen Fibril; Complex; Dental Cementum; Dental caries; Dentin; Dentistry; Event; Funding; Grant; Human; Image; Lesion; Mechanics; Mineral Fibers; Minerals; National Center for Research Resources; Natural regeneration; Operative Surgical Procedures; Periodontal Diseases; Periodontal Ligament; Principal Investigator; Property; Radiation; Research; Research Infrastructure; Resources; Source; Structure; Tissues; Tooth structure; United States National Institutes of Health; Variant; alveolar bone; base; biomineralization; bone; clinically relevant; cost; density; minimally invasive; physical property; remineralization; repaired; structural biology; tissue regeneration

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The clinical relevance in investigating these tissues and interfaces is twofold. Firstly, the challenge in regaining tooth function lost due to periodontal disease lies in encouraging regeneration of critical interfaces that form the tooth attachment apparatus. Effective tissue regeneration requires understanding the union between dissimilar materials such as bone and cementum via the periodontal ligament. In this study we will use TXM and XRF imaging to reveal differences in physical properties in particular structural and mineral variations of healthy and diseased alveolar bone and cementum in humans. The physical properties include observation of degraded collagen fibers, mineral density variations, and mineral and collagen fiber association. Secondly, minimally invasive dentistry seeks repair of carious lesions (tooth decay) as a first step to restoring the damaged tissue. If caries reach dentin, the mineralized collagen-based tissue that forms the bulk of the tooth, decay proceeds at a faster rate and surgical intervention (drill and fill) is required. Due to the complex structure of dentin, it has not been established whether proper mechanical functioning can be restored by remineralization treatments. In this study, using TXM we seek to define structural and property changes associated with dentin remineralization to aid in defining the "quality", or hydrated indentation modulus, of remineralized tissue. TXM images from four identifiable zones of varying mineral content in human dentin carious lesions containing collagen fibrils with interfibrillar apatite nuclei facilitating functional remineralization by regrowth of the apatite crystals will be presented.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 研究这些组织和界面的临床意义是双重的。首先，恢复由于牙周病而丧失的牙齿功能的挑战在于鼓励形成牙齿附着装置的关键界面的再生。 有效的组织再生需要了解不同材料之间的联合，如骨和牙骨质通过牙周韧带。 在这项研究中，我们将使用TXM和XRF成像来揭示人类健康和患病牙槽骨和牙骨质的物理特性差异，特别是结构和矿物变化。 物理性质包括观察降解的胶原纤维、矿物质密度变化以及矿物质和胶原纤维结合。 其次，微创牙科寻求修复龋损（蛀牙）作为恢复受损组织的第一步。 如果龋齿到达牙本质，形成牙齿大部分的矿化胶原基组织，则龋齿以更快的速度进行，并且需要手术干预（钻孔和填充）。由于牙本质的复杂结构，尚未确定牙本质矿化治疗是否可以恢复适当的机械功能。在这项研究中，使用TXM，我们试图定义与牙本质矿化相关的结构和性质变化，以帮助定义矿化组织的“质量”或水合压痕模量。 TXM图像从四个可识别的区域不同的矿物含量在人类牙本质龋损含有胶原纤维与纤维间磷灰石核促进功能性再矿化的磷灰石晶体的再生长。