IDENTIFYING BIOMINERALIZATION EVENTS IN HEALTHY AND DISEASED TISSUES: BONE, DENT

识别健康和患病组织中的生物矿化事件：骨骼、凹痕

• 批准号: 8362403
• 负责人: Sunita P Ho
• 金额: $ 0.03万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362403
• 关键词: Apatites; Bone Tissue; Cell Nucleus; Collagen; Collagen Fiber; Collagen Fibril; Complex; Dental Cementum; Dental caries; Dentin; Dentistry; Event; Funding; Grant; Human; Image; Lesion; Mechanics; Mineral Fibers; Minerals; National Center for Research Resources; Natural regeneration; Operative Surgical Procedures; Periodontal Diseases; Periodontal Ligament; Principal Investigator; Property; Radiation; Research; Research Infrastructure; Resources; Source; Structure; Tissues; Tooth structure; United States National Institutes of Health; Variant; alveolar bone; base; biomineralization; bone; clinically relevant; cost; density; minimally invasive; physical property; remineralization; repaired; structural biology; tissue regeneration

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The clinical relevance in investigating these tissues and interfaces is twofold. Firstly, the challenge in regaining tooth function lost due to periodontal disease lies in encouraging regeneration of critical interfaces that form the tooth attachment apparatus. Effective tissue regeneration requires understanding the union between dissimilar materials such as bone and cementum via the periodontal ligament. In this study we will use TXM and XRF imaging to reveal differences in physical properties in particular structural and mineral variations of healthy and diseased alveolar bone and cementum in humans. The physical properties include observation of degraded collagen fibers, mineral density variations, and mineral and collagen fiber association. Secondly, minimally invasive dentistry seeks repair of carious lesions (tooth decay) as a first step to restoring the damaged tissue. If caries reach dentin, the mineralized collagen-based tissue that forms the bulk of the tooth, decay proceeds at a faster rate and surgical intervention (drill and fill) is required. Due to the complex structure of dentin, it has not been established whether proper mechanical functioning can be restored by remineralization treatments. In this study, using TXM we seek to define structural and property changes associated with dentin remineralization to aid in defining the "quality", or hydrated indentation modulus, of remineralized tissue. TXM images from four identifiable zones of varying mineral content in human dentin carious lesions containing collagen fibrils with interfibrillar apatite nuclei facilitating functional remineralization by regrowth of the apatite crystals will be presented.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 研究这些组织和界面的临床意义是双重的。首先，在恢复因牙周病失去的牙齿功能方面的挑战在于鼓励形成牙齿附着装置的关键界面的再生。有效的组织再生需要了解不同材料之间的结合，如通过牙周膜的骨和牙骨质。在这项研究中，我们将使用TXM和XRF成像来揭示人类健康和患病的牙槽骨和牙骨质的物理性质的差异，特别是结构和矿物质的变化。物理性质包括观察降解的胶原纤维，矿物质密度的变化，以及矿物质和胶原纤维的结合。其次，微创牙科寻求修复龋损(龋齿)，作为修复受损组织的第一步。如果龋齿达到牙本质，即构成牙齿主体的矿化胶原基组织，龋齿会以更快的速度进行，需要手术干预(钻孔和填充)。由于牙本质结构的复杂性，再矿化治疗能否恢复牙本质的机械功能尚不清楚。在这项研究中，我们试图使用TXM来定义与牙本质再矿化相关的结构和性质变化，以帮助定义再矿化组织的“质量”或水化压痕模数。TXM图像来自人类牙本质龋损中四个不同矿物质含量的可识别区域，其中含有胶原纤维和纤维间磷灰石核，通过磷灰石晶体的再生促进功能再矿化。