Functional Competence of a Dentoalveolar Fibrous Joint in Vertebrates

脊椎动物牙槽纤维关节的功能能力

• 批准号: 10245170
• 负责人: Sunita P Ho
• 金额: $ 38.36万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-02-09 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10245170
• 关键词: Age; Animals; Biological; Biological Assay; Biological Process; Biomechanics; Cells; Clinical; Competence; Complex; Data; Dental Cementum; Dentists; Detection; Diet; Electron Microscopy; Equilibrium; Exposure to; Failure; Female; Fluorescence; Food; Frequencies; Genes; Guidelines; Human; Image; In Situ; Intervention; Jaw; Joints; Lead; Maps; Mastication; Maxilla; Measures; Mediating; Methods; Minerals; Modeling; Molecular; Morphology; Mus; Muscle; Musculoskeletal System; Orthodontic; Orthodontics; Outcome; Parents; Pathologic; Periodontal Ligament; Phenotype; Physiological; Process; Property; Proteins; Rattus; Recovery; Recovery of Function; Research; Risk; Rodent; Scanning; Site; Soft Diet; Stimulus; Stress; Structure; TNFSF11 gene; Therapeutic; Time; Tissues; Tooth Crowns; Tooth Movement; Tooth structure; Transgenic Mice; Vertebrates; X-Ray Computed Tomography; age group; age related; alveolar bone; asporin; bone; differential expression; digital; in vivo; joint function; joint stiffness; male; mouse model; preservation; response; scleraxis; spatiotemporal; teeth clenching; transmission process

ABSTRACT A variety of biomechanical stimuli are directed on the dentoalveolar fibrous joint (DAJ) of the masticatory complex (e.g. chewing on softer diet (SD), therapeutic loads from orthodontic interventions), and can result in local abnormal deformations and adaptive processes that can lead to aberrant function [1-7]. A systematic study of the effect of SD on the tooth-crown revealed significant age-related shifts in biological processes in the periodontal complex (R01DE022032 02/2012-01/2018). Thus, recovery of an adapting joint to its original stiffness is compromised with age, but the question remained: when the joint is exposed to prolonged aberrant loads, what is the optimal age range for the joint to recover to its baseline biomechanics should the aberrant load be substituted with physiologic loads (hard diet (HD))? What is the age range beyond which joint biomechanics are irreversible? Data from the recovery model (RM) (mice raised to various time points on SD and switched to HD), will help determine the age range for which functional competence of the joint is maintained, and help predict when clinical intervention simulated through an experimental tooth movement (ETM) model is most effective. ETM will also help in identifying the intended reversal of natural biological processes [8] at the strain-amplified sites of the PDL-entheses. Hypothesis: The functional competence of the DAJ can be identified as age-related gradients in biological expressions (ΔBE/Δage) and joint stiffness (ΔS/Δage). From a translational perspective, ETM is effective in an age range that demonstrates minima in ΔS/Δage. Proposed aims will include: Aim 1: To assess the recovery of functional competence of adapting fibrous joints with age. Functional competence of an adapted joint will be determined by its ability to resist shifts in magnitudes and frequencies of biomechanical loads without subsequent pathologic function [9]; to-date no such data exist. Functional competence will be determined from digital spatiotemporal maps of changes in periodontal ligament (PDL)-spaces, tooth-bone morphology and joint stiffness (ΔS/Δage) with age from SD, HD, RM groups. The RM will identify an age range to optimize preservation of the fibrous joint. Aim 2: To assess the mechanobiological processes of an adapting fibrous joint with age. Spatiotemporal shifts in biological expressions (BE) (ΔBE/Δage – genes and matrix proteins) at the bone, cementum, and the PDL-entheses from respective age groups will be mapped. Differentially expressed genes, and mineral forming and resorbing matrix factors at PDL-entheses and the PDL at widened and narrowed regions with age for three groups (SD, HD, RM) will be correlated. AIM 3: To assess the effect of a proposed age range on experimental tooth movement resulting in long-term functional competence. Outcome physicochemical and biological measures will be the same as in Aims 1 and 2 but on ETM group [10] before, during, and after the proposed age range from the RM in Aims 1 and 2. The intended reversal of natural biological process at the strain-amplified PDL-entheses in the ETM model also will be investigated.

摘要 各种生物力学刺激作用于咀嚼牙槽骨纤维关节(DAJ) 复杂(例如，咀嚼较软的饮食(SD)，正畸干预带来的治疗负荷)，并可能导致 局部异常变形和适应过程可导致功能异常[1-7]。一个系统的 对SD对牙冠影响的研究揭示了与年龄相关的生物过程在 牙周复合体(R01DE022032 02/2012-01/2018)。因此，适配关节恢复到其原始状态 僵硬会随着年龄的增长而降低，但问题仍然是：关节何时暴露于长期的异常 如果出现异常，关节恢复到基线生物力学的最佳年龄范围是多少 用生理负荷代替负荷(硬性饮食(HD))？超过哪个关节的年龄范围是多少？ 生物力学是不可逆转的吗？来自恢复模型(RM)的数据(SD饲养到不同时间点的小鼠 并切换到HD)，将有助于确定关节功能能力的年龄范围 维护并帮助预测通过实验性牙齿移动模拟临床干预的时间 (ETM)模式最为有效。ETM还将帮助确定自然生物的预期逆转 在PDL包膜的应变扩增部位进行处理[8]。假设：人的功能能力 在生物表达(ΔBE/ΔAGE)和关节中，DAJ可以被识别为与年龄相关的梯度 刚性(ΔS/Δ年龄)。从翻译的角度来看，ETM在一个年龄段内是有效的，这表明 ΔS/Δ时代的极小值。拟议的目标将包括：目标1：评估功能能力的恢复情况 随着年龄的增长，适应纤维关节。适应关节的功能能力将取决于其能力。 在没有后续病理功能的情况下抵抗生物力学载荷的大小和频率的变化 [9]；到目前为止还没有这样的数据。功能能力将根据数字时空地图确定 牙周膜间隙、牙-骨形态和关节硬度随年龄的变化(ΔS/Δ年龄) 来自SD、HD、RM组。RM将确定一个年龄范围，以优化纤维关节的保存。目标 2：评估适应的纤维关节的力学生物学过程随年龄的变化。时空 骨、牙骨质和骨组织中生物表达(BE)(ΔBE/ΔAGE基因和基质蛋白)的变化 将映射来自各个年龄段的PDL-enet。差异表达基因与矿物形成 随着年龄的增长，在PDL末端重新吸收基质因子，在加宽和狭窄区域重新吸收PDL 组(SD、HD、RM)将相互关联。目标3：评估建议的年龄范围对 实验性牙齿移动导致长期的功能能力。结果 物理化学和生物措施将与目标1和目标2相同，但在ETM组[10] 在目标1和目标2中建议的年龄范围之前、期间和之后。 在ETM模型中，还将研究菌株扩增PDL末端的自然生物过程。

项目成果

Contact ratio and adaptations in the maxillary and mandibular dentoalveolar joints in rats and human clinical analogs.

大鼠和人类临床类似物上颌和下颌牙槽关节的接触比和适应性。

• DOI: 10.1016/j.jmbbm.2022.105485
• 发表时间: 2022
• 期刊: Journal of the mechanical behavior of biomedical materials
• 影响因子: 3.9
• 作者: Wang,Bo;Nguyen,Nam;Kang,Misun;Srirangapatanam,Sudarshan;Connelly,Stephen;Souza,Richard;Ho,SunitaP
• 通讯作者: Ho,SunitaP

Food hardness can regulate orthodontic tooth movement in mice.

食物硬度可以调节小鼠正畸牙齿的移动。

• DOI: 10.1111/jre.12945
• 发表时间: 2022
• 期刊: Journal of periodontal research
• 影响因子: 3.5
• 作者: Ustriyana,Putu;He,Rui;Srirangapatanam,Sudarshan;Chang,Jasper;Arman,SheelerT;Sidhu,Sukhmandeep;Wang,Bo;Kang,Misun;Ho,SunitaP
• 通讯作者: Ho,SunitaP

Microanatomical changes and biomolecular expression at the PDL-entheses during experimental tooth movement.

实验性牙齿移动过程中 PDL 附着点的显微解剖变化和生物分子表达。

• DOI: 10.1111/jre.12625
• 发表时间: 2019
• 期刊: Journal of periodontal research
• 影响因子: 3.5
• 作者: Yang,Lynn;Kang,Misun;He,Rui;Meng,Bo;Pal,Arvin;Chen,Ling;Jheon,AndrewH;Ho,SunitaP
• 通讯作者: Ho,SunitaP

Functional adaptation of interradicular alveolar bone to reduced chewing loads on dentoalveolar joints in rats.

• DOI: 10.1016/j.dental.2020.12.003
• 发表时间: 2021-03
• 期刊: Dental materials : official publication of the Academy of Dental Materials
• 作者: Jang A;Wang B;Ustriyana P;Gansky SA;Maslenikov I;Useinov A;Prevost R;Ho SP
• 通讯作者: Ho SP

Differentiating zones at periodontal ligament-bone and periodontal ligament-cementum entheses.

• DOI: 10.1111/jre.12281
• 发表时间: 2015-12
• 期刊: Journal of periodontal research
• 影响因子: 3.5
• 作者: Lee JH;Pryce BA;Schweitzer R;Ryder MI;Ho SP
• 通讯作者: Ho SP