Pressure-inducer and Sensor to Map Dynamic Periodontal Mechanobiological Activity

压力感应器和传感器绘制动态牙周机械生物学活动

• 批准号: 9376394
• 负责人: Sunita P Ho
• 金额: $ 23.78万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-01 至 2019-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9376394
• 关键词: Adopted; Alkaline Phosphatase; Alteplase; Alveolar Bone Loss; Animals; Biological; Biological Process; Biomedical Engineering; Biosensor; Clinic; Clinical; Complex; Consensus; Couples; Data; Dental crowns; Dimensions; Effectiveness; Encapsulated; Event; Excision; Functional Imaging; Future; Goals; Imagery; Imaging Techniques; In Situ; Intervention; Maps; Mastication; Measurable; Measures; Microscopy; Minerals; Modeling; Molar tooth; Monitor; Movement; Nature; Orthodontic; Oryctolagus cuniculus; Patients; Periodontal Ligament; Phase; Plant Roots; Positioning Attribute; Proteins; Recovery; Relapse; Rest; Roentgen Rays; Silicon; Silicones; System; TRANCE protein; Technology; Testing; Thick; Time; Tooth Crowns; Tooth Extraction; Tooth Movement; Tooth Socket; Tooth structure; Translating; Translations; X-Ray Computed Tomography; alveolar bone; asporin; biglycan; biomaterial compatibility; bone; bone sialoprotein; clinical application; digital; experimental study; in vivo; in vivo Model; insight; interdisciplinary approach; millimeter; pre-clinical; pressure; response; scleraxis; sensor; spatiotemporal; tartrate-resistant acid phosphatase

ABSTRACT The proposed interdisciplinary approach is to test the functionality of an elastic sensor that will both create and measure pressure on molar teeth in a preclinical rabbit model. Sensitivity of the commercially available sub-millimeter capacitive silicon pressure sensor encapsulated in silicone will be defined as the ability to monitor temporal shifts in pressures/forces on the tooth-crown during short- and long-term phases of tooth movements. Scientific Premise: In orthodontic tooth movement (OTM), the mineral formation and resorption events caused by natural tooth drift in the periodontal complex are often reversed through the time dependent mechanoresponsive nature of the PDL. This is achieved through largely qualitative adjustments of the magnitude and direction of forces and couples on the tooth crowns during clinical manipulations. With a calibrated force on the tooth-crown, preliminary insights have led us to hypothesize that the temporal sensitivity of the proposed biosensor can be mapped by identifying the gradual rate of shift in pressure (∆Pg/∆t). These shifts will prompt gradual reversal of the naturally occurring mineral resorption and formation at the PDL-entheses resulting in minimum tooth recovery/relapse toward their original position. An abrupt rate of shift in pressure (∆Pa/∆t) will be indicative of an abrupt reversal of these biological events and significant tooth relapse. The proposed two aims will include; Aim 1. To investigate the sensitivity of an elastic sensor between the 1st and 2nd molars in a rabbit model. Temporal shifts in pressure on the tooth-crown will be correlated with the functional relationship of the root with the alveolar bone, and changes in PDL-space during short- (t=0, 1 day (D)) and long-term (1 week (wk) and 2 weeks (wks)) phases of OTM, as well as tooth recovery/relapse. Aim 2. To investigate the functional effectiveness of an elastic sensor between 1st and 2nd molars of a rabbit. Temporal shifts in the measured in vivo input pressure at different thicknesses of the elastic sensor (relative to PDL-space) will be mapped and correlated with resulting biological responses. Three types of sensor thicknesses (T) will be used: 1) equivalent to PDL-space (T~average PDL-space in a rabbit: 70-100 µm), 15-20% lower (T<PDL-space) and 15-20% higher than average PDL-space (T>PDL-space) with time points similar to Aim 1. This combination of readily available micro-electro-mechanical system (MEMS) and simple fabrication into a pressure sensor will allow early efforts to be focused on proof-of-concept experiments utilizing in vivo models with the future goal of application in orthodontic patients in the clinic. This study will enable precise quantification of force modulation on tooth-crowns (Aim 1) by correlating with biological processes (Aim 2), that will facilitate optimal OTM with minimum tooth recovery/relapse. This data will help translate the proposed technology to clinical applications especially during short- and long-term phases of OTM intervention.

摘要 所提出的跨学科方法是测试弹性传感器的功能， 并在临床前兔模型中测量臼齿上的压力。市售产品的灵敏度 封装在硅树脂中的亚毫米电容式硅压力传感器将被定义为能够 在牙齿的短期和长期阶段期间监测牙冠上的压力/力的时间变化 动作科学假设：在正畸牙齿移动（OTM）中，矿物质的形成和吸收 由牙周复合体中的自然牙齿漂移引起的事件通常通过时间依赖性的 PDL的机械反应性质。这主要是通过质量调整实现的， 在临床操作过程中，牙冠上的力和力偶的大小和方向。与 校准力的牙冠，初步的见解使我们假设， 所提出的生物传感器的灵敏度可以通过识别 压力（表压/吨）。这些变化将促使天然矿物逐渐逆转 PDL-附着点处的吸收和形成，导致最小的牙齿恢复/复发， 原来的位置。压力的突然变化率（kPa/dt）将指示压力的突然变化。 这些生物学事件的逆转和显著的牙齿复发。拟议的两个目标将包括： 1.目的：研究在兔第一、二磨牙间植入弹性传感器的敏感性。 牙冠上压力的时间变化将与牙根的功能关系相关， 牙槽骨，以及PDL-间隙在短期（t=0，1天（D））和长期（1周（wk）和2 周（wks））的OTM阶段，以及牙齿恢复/复发。目标2.为了研究功能性 兔第一、二磨牙间弹性传感器的有效性测量的时间变化 将绘制弹性传感器的不同厚度（相对于PDL空间）下的体内输入压力， 与所产生的生物反应相关。将使用三种类型的传感器厚度（T）：1） 相当于PDL-空间（T~家兔的平均PDL-空间：70-100 µm），低15-20%（T<PDL-空间），以及 比平均PDL-空间高15-20%（T> PDL-空间），时间点与目标1相似。的这种组合 容易获得的微机电系统（MEMS）和简单制造成压力传感器将 允许早期的努力集中在利用体内模型的概念验证实验上，未来目标是 在正畸患者中的临床应用。这项研究将使精确的量化力调制 通过与生物过程（目标2）相关联，在牙冠（目标1）上， 最小的牙齿恢复/复发。这些数据将有助于将拟议的技术转化为临床应用 特别是在OTM干预的短期和长期阶段。