Pressure-inducer and Sensor to Map Dynamic Periodontal Mechanobiological Activity

压力感应器和传感器绘制动态牙周机械生物学活动

• 批准号: 9376394
• 负责人: Sunita P Ho
• 金额: $ 23.78万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-01 至 2019-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9376394
• 关键词: Adopted; Alkaline Phosphatase; Alteplase; Alveolar Bone Loss; Animals; Biological; Biological Process; Biomedical Engineering; Biosensor; Clinic; Clinical; Complex; Consensus; Couples; Data; Dental crowns; Dimensions; Effectiveness; Encapsulated; Event; Excision; Functional Imaging; Future; Goals; Imagery; Imaging Techniques; In Situ; Intervention; Maps; Mastication; Measurable; Measures; Microscopy; Minerals; Modeling; Molar tooth; Monitor; Movement; Nature; Orthodontic; Oryctolagus cuniculus; Patients; Periodontal Ligament; Phase; Plant Roots; Positioning Attribute; Proteins; Recovery; Relapse; Rest; Roentgen Rays; Silicon; Silicones; System; TRANCE protein; Technology; Testing; Thick; Time; Tooth Crowns; Tooth Extraction; Tooth Movement; Tooth Socket; Tooth structure; Translating; Translations; X-Ray Computed Tomography; alveolar bone; asporin; biglycan; biomaterial compatibility; bone; bone sialoprotein; clinical application; digital; experimental study; in vivo; in vivo Model; insight; interdisciplinary approach; millimeter; pre-clinical; pressure; response; scleraxis; sensor; spatiotemporal; tartrate-resistant acid phosphatase

ABSTRACT The proposed interdisciplinary approach is to test the functionality of an elastic sensor that will both create and measure pressure on molar teeth in a preclinical rabbit model. Sensitivity of the commercially available sub-millimeter capacitive silicon pressure sensor encapsulated in silicone will be defined as the ability to monitor temporal shifts in pressures/forces on the tooth-crown during short- and long-term phases of tooth movements. Scientific Premise: In orthodontic tooth movement (OTM), the mineral formation and resorption events caused by natural tooth drift in the periodontal complex are often reversed through the time dependent mechanoresponsive nature of the PDL. This is achieved through largely qualitative adjustments of the magnitude and direction of forces and couples on the tooth crowns during clinical manipulations. With a calibrated force on the tooth-crown, preliminary insights have led us to hypothesize that the temporal sensitivity of the proposed biosensor can be mapped by identifying the gradual rate of shift in pressure (∆Pg/∆t). These shifts will prompt gradual reversal of the naturally occurring mineral resorption and formation at the PDL-entheses resulting in minimum tooth recovery/relapse toward their original position. An abrupt rate of shift in pressure (∆Pa/∆t) will be indicative of an abrupt reversal of these biological events and significant tooth relapse. The proposed two aims will include; Aim 1. To investigate the sensitivity of an elastic sensor between the 1st and 2nd molars in a rabbit model. Temporal shifts in pressure on the tooth-crown will be correlated with the functional relationship of the root with the alveolar bone, and changes in PDL-space during short- (t=0, 1 day (D)) and long-term (1 week (wk) and 2 weeks (wks)) phases of OTM, as well as tooth recovery/relapse. Aim 2. To investigate the functional effectiveness of an elastic sensor between 1st and 2nd molars of a rabbit. Temporal shifts in the measured in vivo input pressure at different thicknesses of the elastic sensor (relative to PDL-space) will be mapped and correlated with resulting biological responses. Three types of sensor thicknesses (T) will be used: 1) equivalent to PDL-space (T~average PDL-space in a rabbit: 70-100 µm), 15-20% lower (T<PDL-space) and 15-20% higher than average PDL-space (T>PDL-space) with time points similar to Aim 1. This combination of readily available micro-electro-mechanical system (MEMS) and simple fabrication into a pressure sensor will allow early efforts to be focused on proof-of-concept experiments utilizing in vivo models with the future goal of application in orthodontic patients in the clinic. This study will enable precise quantification of force modulation on tooth-crowns (Aim 1) by correlating with biological processes (Aim 2), that will facilitate optimal OTM with minimum tooth recovery/relapse. This data will help translate the proposed technology to clinical applications especially during short- and long-term phases of OTM intervention.

抽象的 拟议的跨学科方法是测试弹性传感器的功能，这两个传感器都会创建 并测量临床前兔模型中摩尔牙齿的压力。市售的敏感性 封装在硅酮中的亚毫米电容硅压力传感器将定义为 在短期和长期牙齿的牙齿上，监测牙齿冠上压力/力的暂时变化 动作。科学前提：在正畸牙齿运动（OTM）中，矿物形成和分辨率 牙周综合体中自然牙齿漂移引起的事件通常会在时间上逆转 PDL的机械响应性质。这是通过在很大程度上对 临床操纵期间，力和夫妻在牙齿冠上的大小和方向。与 牙冠上的校准力，初步见解使我们假设临时 可以通过识别鉴定等级的变化率来映射所提出的生物传感器的敏感性 压力（∆PG/∆T）。这些转变将促使自然发生的未成年人的成绩逆转 PDL-渗透的分辨率和形成，导致最小的牙齿回收/复发 他们的原始位置。压力的突然移动率（∆Pa/∆t）将表明突然 逆转这些生物事件和大量牙齿释放。拟议的两个目标将包括：目的 1。研究兔模型中第一和第二磨牙之间弹性传感器的灵敏度。 牙冠压力的时间变化将与根与根的功能关系相关 牙槽骨和短期（t = 0，1天（d））和长期（1周（WK）和2的PDL空间变化 OTM的几周（WKS））以及牙齿回收/复发。目标2。调查功能 兔第一和第二磨牙之间的弹性传感器的有效性。测得的时间变化 在弹性传感器不同厚度（相对于PDL空间）的不同厚度下的体内输入压力将被映射，并且 与产生的生物学反应相关。将使用三种类型的传感器厚度（T）：1） 相当于PDL空间（兔子中的平均PDL空间：70-100 µm），低15-20％（T <PDL空间）和 比平均PDL空间（T> PDL空间）高15-20％，时间点类似于AIM 1。 随时可用的微电动机电系统（MEMS）和简单制造为压力传感器 允许早期努力专注于使用体内模型的概念证明实验，其未来目标的目标 在诊所的正畸患者中应用。这项研究将对力调制进行精确量化 通过与生物过程相关（AIM 2），在牙齿冠上（AIM 1），这将促进最佳OTM 最小牙齿回收/复发。这些数据将有助于将拟议的技术转化为临床应用 特别是在OTM干预的短期和长期阶段。