High Resolution Non-Invasive Imaging of Oral and Craniofacial Tissues

口腔和颅面组织的高分辨率非侵入性成像

• 批准号: 7793331
• 负责人: Sunita P Ho
• 金额: $ 50万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-15 至 2011-07-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7793331
• 关键词: Applications Grants; Area; Arts; Biocompatible Materials; Biological; Biological Sciences; Biomedical Engineering; Biomimetics; Bone Diseases; Characteristics; Chemicals; Clinical; Complex; Core Facility; Dental; Dental caries; Dentistry; Development; Diagnostic Procedure; Dimensions; Disease; Equipment; Evaluation; Faculty; Funding; Future; Generations; Grant; Health; Housing; Image; In Situ; Interdisciplinary Study; Laboratory Personnel; Maintenance; Methods; Minerals; Minor; Names; Natural regeneration; Operative Surgical Procedures; Oral; Preventive; Research; Research Personnel; Resolution; Roentgen Rays; Scanning Electron Microscopy; School Dentistry; Science; Simulate; Stem cells; Structure; System; Techniques; Time; Tissue Engineering; Tissues; Training Support; United States National Institutes of Health; Work; X-Ray Computed Tomography; base; craniofacial; implantation; in vivo; instrument; nanoscale; novel diagnostics; operation; programs; public health relevance; remineralization; restoration; skeletal; soft tissue; therapy development; tissue regeneration

DESCRIPTION (provided by applicant): This application is for acquisition of a micro X-ray computed tomograph (MicroXCT-200). Recent advances in computed tomography open new opportunities for biomaterials and bioengineering research. The developments of microCTs allow high resolution imaging of internal structures of mineralized tissues and their interfaces with soft tissues down to 1 <m under in situ conditions (simulated in vivo). These capabilities are a breakthrough in the analysis of biological tissues and biomaterials to understand their native characteristics which constitute an integral part in the development of new generation of smart materials for restoration, implantation and tissue regeneration. The MicroXCT-200 is one of the most versatile instruments for a quantitative microstructural and mineral content evaluation in three spatial dimensions. This multiuser facility will greatly enhance the research of seven NIH investigators, as well as several other full-time researchers. The microCTs facilities currently available on campus are limited in resolution with 100% usage and much less capable. Funding is requested for the acquisition of a state-of-the-art MicroXCT-200, Xradia Inc., system. The proposal has received broad based support from UCSF, SFGH, UOP, LBNL and SLAC faculty; personnel from laboratory groups representing 8 PI's with current funding from NIH as projected users. The proposed MicroXCT-200 will be housed in the Nanoscale Tissue Characterization Core Facility in the Division of Biomaterials and Bioengineering, Dept. of Preventive and Restorative Dental Sciences, School of Dentistry, UCSF. The core facility in the Division of B&B has championed interdisciplinary research with the programs in the life sciences, materials sciences, and chemical sciences using high resolution atomic force and scanning electron microscopy techniques and will guarantee maintenance and operation of the instrument in top condition, and also will provide excellent training and support for the major users named on the grant proposal, and minor and future users. Acquisition of this instrument will promote substantial progress on current grants that include understanding of the periodontal complex in health and disease, bone disease, dental caries, and methods of remineralization, tissue regeneration using biomimetics and tissue engineering principles, and stem cell approaches, as well as the development of non-ionizing imaging for clinical dentistry for the projects to be studied. PUBLIC HEALTH RELEVANCE: This grant requests new high magnification non-invasive imaging equipment to support critical work of a number of NIH supported investigators who are working in the areas of dental and bone disease and the development of treatments that will minimize needed surgery, new diagnostic procedures for dental decay, and tissue engineering and stem cells to aid in regeneration of dental, oral, and skeletal issues.

描述（由申请人提供）：本申请是为了获取微型x射线计算机断层扫描仪（MicroXCT-200）。计算机断层扫描的最新进展为生物材料和生物工程研究开辟了新的机会。微型ct的发展允许在原位条件下（体内模拟）对矿化组织的内部结构及其与软组织的界面进行1 <m的高分辨率成像。这些能力是生物组织和生物材料分析的一个突破，可以了解它们的天然特性，这是开发用于修复、植入和组织再生的新一代智能材料的一个组成部分。MicroXCT-200是三维空间定量显微结构和矿物含量评估最通用的仪器之一。这个多用户设施将极大地加强七名NIH研究人员以及其他几名全职研究人员的研究。目前校园内可用的微型ct设备分辨率有限，不能达到100%的使用率，而且功能也很差。申请资金用于采购Xradia公司最先进的MicroXCT-200系统。该提案得到了UCSF， SFGH， UOP， LBNL和SLAC教师的广泛支持；来自实验室小组的人员代表8个PI，目前由NIH提供资金作为预期用户。拟议中的MicroXCT-200将被安置在UCSF牙科学院预防和修复牙科科学系生物材料和生物工程系的纳米级组织表征核心设施中。B&B部门的核心设施支持生命科学，材料科学和化学科学的跨学科研究项目，使用高分辨率原子力和扫描电子显微镜技术，并保证仪器在最佳状态下的维护和运行，同时也将为资助提案中指定的主要用户，以及次要和未来的用户提供出色的培训和支持。获得这一仪器将促进目前赠款的重大进展，包括了解健康和疾病中的牙周复合物、骨病、龋齿和再矿化方法、利用仿生学和组织工程原理进行组织再生以及干细胞方法，以及为将要研究的项目开发临床牙科非电离成像技术。

项目成果

Architecture-Guided Fluid Flow Directs Renal Biomineralization.

• DOI: 10.1038/s41598-018-30717-x
• 发表时间: 2018-09-21
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Ho SP;Chen L;Allen FI;Hsi RS;Shimotake AR;Wiener SV;Kang M;Minor AM;Stoller ML
• 通讯作者: Stoller ML

The Role of Epithelial Stat3 in Amelogenesis during Mouse Incisor Renewal.

• DOI: 10.1159/000486745
• 发表时间: 2018
• 期刊: Cells, tissues, organs
• 作者: Zhang B;Meng B;Viloria E;Naveau A;Ganss B;Jheon AH
• 通讯作者: Jheon AH

Biomechanics and strain mapping in bone as related to immediately-loaded dental implants.

• DOI: 10.1016/j.jbiomech.2015.05.014
• 发表时间: 2015-09-18
• 期刊: Journal of biomechanics
• 影响因子: 2.4
• 作者: Du J;Lee JH;Jang AT;Gu A;Hossaini-Zadeh M;Prevost R;Curtis DA;Ho SP
• 通讯作者: Ho SP

Fluorosed mouse ameloblasts have increased SATB1 retention and Gαq activity.

• DOI: 10.1371/journal.pone.0103994
• 发表时间: 2014
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Zhang Y;Kim JY;Horst O;Nakano Y;Zhu L;Radlanski RJ;Ho S;Den Besten PK
• 通讯作者: Den Besten PK

A continuum of mineralization from human renal pyramid to stones on stems.

从人类肾金字塔到茎上的石头的矿化连续体。

• DOI: 10.1016/j.actbio.2018.01.040
• 发表时间: 2018
• 期刊: Acta biomaterialia
• 影响因子: 9.7
• 作者: Sherer,BenjaminA;Chen,Ling;Kang,Misun;Shimotake,AlexR;Wiener,ScottV;Chi,Tom;Stoller,MarshallL;Ho,SunitaP
• 通讯作者: Ho,SunitaP