Biomimetic Properties of Cementum and Its Interfaces

牙骨质及其界面的仿生特性

• 批准号: 7932570
• 负责人: Sunita P Ho
• 金额: $ 3.26万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-22 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7932570
• 关键词: Accounting; Address; Age; Animal Model; Anterior; Apical; Area; Arts; Atomic Force Microscopy; Award; Binding; Biocompatible Materials; Biological; Biomechanics; Biomedical Engineering; Biomimetics; Bone Resorption; Cadaver; Cell Adhesion; Cell Proliferation; Cell division; Cervix Uteri; Characteristics; Chemical Structure; Chemicals; Chondroitin ABC Lyase; Chondroitin Sulfate A; Chondroitin Sulfate C; Collagen; Collagen Fibril; Collagen Type I; Complex; Connective Tissue; Cytoskeleton; Data; Data Collection; Dehydration; Dental; Dental Cementum; Dental Enamel; Dentin; Dermatan Sulfate; Disease; Disease Progression; Engineering; Environment; Enzymes; Faculty; Female; Gender; Glycosaminoglycans; Goals; Guidelines; Hardness; Human; Hydration status; Image; Incisor; Inflammation; Interferometry; Investigation; Journals; Keratan Sulfate; Knowledge; Location; Manuscripts; Maps; Matrix Metalloproteinases; Measures; Mechanics; Memory; Mentors; Minerals; Modeling; Molar tooth; Natural regeneration; Pathway interactions; Periodontal Diseases; Periodontal Ligament; Periodontitis; Periodontium; Phase; Plant Roots; Procedures; Property; Proteoglycan; Publishing; Rattus; Research; Research Personnel; Shapes; Shock; Specimen; Structure; Techniques; Testing; Time; Tissue Engineering; Tissues; Tooth Loss; Tooth structure; United States National Institutes of Health; Variant; Width; Work; age group; alveolar bone; animal tissue; base; bone; cell behavior; cell growth; chondroitinase B; crosslink; hydrophilicity; keratan-sulfate endo-1,4-b-galactosidase; male; member; nanoscale; next generation; novel; oral tissue; programs; pyridinoline; response; scaffold; tissue regeneration

The overall purpose of the NIH Pathway to Independence Award K99/ROO application is to provide a year of support for the mentored phase that is crucial in completing the critical testing of four hypotheses, followed by manuscript submission to relevant journals. The subsequent 3 year independent phase is necessary to establish my research program and reaching my goals as a junior faculty member. It would enable me to establish myself as an independent investigator in biomaterials integration and characterization taking into account both human and comparable animal tissues, subsequently building tissues with well optimized bioengineered interfaces. This award will enable me to study the proposed specific aims defined in this proposal and expand my knowledge on building the next generation materials for tissue regeneration. The thrust of these efforts is based on a general hypothesis that the functional biomechanics of a tooth are derived from structural and chemical interactions of its different components at several hierarchical levels (macro-, micro- and nano-scales), and that these yield properties that depend on location, age, and gender. Destruction of tissues including the periodontal ligament (PDL), cementum, and bone can cause loss of teeth due to periodontitis. Key challenges include 1). Understanding degradation of the tissues associated with disease progression, and 2). Regeneration of the interfaces that bind the oral tissues together. To address the first challenge, an animal model for periodontitis will be developed and the sequential degeneration of tissues determined by studying their structure, chemical composition and mechanical properties. For the second challenge, tissue engineering (TE) can be used to create novel scaffolds. However, a major limitation of current TE procedures is limited knowledge of scaffold biomechanics. An efficient scaffold should sustain functional loads in addition to providing an environment conducive to desired cell behavior. To address these challenges, the inherent characteristics of the tissues and their interfaces must be determined. Then, they can be mimicked using TE to create appropriate scaffolds. Hence the following specific aims are defined to: 1). Investigate structure, chemical composition, mechanical properties of primary and secondary cementums, 2). Investigate structure, chemical composition, mechanical properties of cementum and its interface with root dentin, 3). Investigate strain fields using functional loads on anterior and posterior teeth as a function of age, 4). Perform a comparison studies between structure, chemical composition and mechanical properties of healthy human and rat alveolar bone, PDL, cementum, root dentin and their bimaterial interfaces. This information will be used to determine chronological changes in structure, chemical composition and mechanical properties in the rat periodontal tissues and their bimaterial interfaces during disease progression.

NIH通向独立奖K99/Roo应用程序的总体目的是提供 支持指导阶段的一年，这一阶段对完成四个假设的关键检验至关重要， 随后向相关期刊投稿。接下来的3年独立阶段是 有必要建立我的研究计划，并实现我作为初级教员的目标。它会 使我成为生物材料集成和表征领域的独立研究员 考虑到人类和类似的动物组织，随后用井建造组织 优化的生物工程接口。这一奖项将使我能够研究所提出的明确的目标 在这项建议中，并扩大我对构建下一代组织再生材料的知识。 这些努力的主旨是基于一个普遍的假设，即牙齿的功能生物力学是 源自其不同成分在几个层次上的结构和化学相互作用 (宏观、微观和纳米尺度)，这些特性取决于地点、年龄和性别。 破坏包括牙周韧带(PDL)、牙骨质和骨组织在内的组织会导致牙周组织的缺失 牙周炎引起的牙齿。主要挑战包括1)。了解相关组织的降解 随着疾病的进展，和2)。将口腔组织结合在一起的界面的再生。至 解决第一个挑战，将开发一种牙周炎动物模型，并按顺序 通过研究组织的结构、化学成分和机械性能而确定的组织退化 属性。对于第二个挑战，可以使用组织工程(TE)来创建新的支架。 然而，目前TE手术的一个主要限制是对支架生物力学的了解有限。一个 高效的脚手架除了提供有利于所需的环境外，还应承受功能负荷 细胞行为。为了应对这些挑战，组织及其界面的固有特性 必须下定决心。然后，可以使用TE模拟它们来创建适当的脚手架。因此， 具体目标如下：1)。研究结构、化学成分、机械性能 原生和次生胶结物的性质，2)。研究结构，化学成分， 牙骨质及其与根面牙本质界面的力学性能，3)调查应变场使用 前牙和后牙的功能负荷与年龄的关系，4)。进行比较研究 健康人和大鼠牙槽骨的结构、化学成分和力学性能之间的关系 牙周膜、牙骨质、根部牙本质及其双材料界面。这些信息将被用来确定 大鼠牙周组织结构、化学成分和力学性能的时间变化 疾病进展过程中的组织及其双材料界面。