Biomimetic Properties of Cementum and Its Interfaces

牙骨质及其界面的仿生特性

• 批准号: 7932570
• 负责人: Sunita P Ho
• 金额: $ 3.26万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-22 至 2010-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7932570
• 关键词: Accounting; Address; Age; Animal Model; Anterior; Apical; Area; Arts; Atomic Force Microscopy; Award; Binding; Biocompatible Materials; Biological; Biomechanics; Biomedical Engineering; Biomimetics; Bone Resorption; Cadaver; Cell Adhesion; Cell Proliferation; Cell division; Cervix Uteri; Characteristics; Chemical Structure; Chemicals; Chondroitin ABC Lyase; Chondroitin Sulfate A; Chondroitin Sulfate C; Collagen; Collagen Fibril; Collagen Type I; Complex; Connective Tissue; Cytoskeleton; Data; Data Collection; Dehydration; Dental; Dental Cementum; Dental Enamel; Dentin; Dermatan Sulfate; Disease; Disease Progression; Engineering; Environment; Enzymes; Faculty; Female; Gender; Glycosaminoglycans; Goals; Guidelines; Hardness; Human; Hydration status; Image; Incisor; Inflammation; Interferometry; Investigation; Journals; Keratan Sulfate; Knowledge; Location; Manuscripts; Maps; Matrix Metalloproteinases; Measures; Mechanics; Memory; Mentors; Minerals; Modeling; Molar tooth; Natural regeneration; Pathway interactions; Periodontal Diseases; Periodontal Ligament; Periodontitis; Periodontium; Phase; Plant Roots; Procedures; Property; Proteoglycan; Publishing; Rattus; Research; Research Personnel; Shapes; Shock; Specimen; Structure; Techniques; Testing; Time; Tissue Engineering; Tissues; Tooth Loss; Tooth structure; United States National Institutes of Health; Variant; Width; Work; age group; alveolar bone; animal tissue; base; bone; cell behavior; cell growth; chondroitinase B; crosslink; hydrophilicity; keratan-sulfate endo-1,4-b-galactosidase; male; member; nanoscale; next generation; novel; oral tissue; programs; pyridinoline; response; scaffold; tissue regeneration

The overall purpose of the NIH Pathway to Independence Award K99/ROO application is to provide a year of support for the mentored phase that is crucial in completing the critical testing of four hypotheses, followed by manuscript submission to relevant journals. The subsequent 3 year independent phase is necessary to establish my research program and reaching my goals as a junior faculty member. It would enable me to establish myself as an independent investigator in biomaterials integration and characterization taking into account both human and comparable animal tissues, subsequently building tissues with well optimized bioengineered interfaces. This award will enable me to study the proposed specific aims defined in this proposal and expand my knowledge on building the next generation materials for tissue regeneration. The thrust of these efforts is based on a general hypothesis that the functional biomechanics of a tooth are derived from structural and chemical interactions of its different components at several hierarchical levels (macro-, micro- and nano-scales), and that these yield properties that depend on location, age, and gender. Destruction of tissues including the periodontal ligament (PDL), cementum, and bone can cause loss of teeth due to periodontitis. Key challenges include 1). Understanding degradation of the tissues associated with disease progression, and 2). Regeneration of the interfaces that bind the oral tissues together. To address the first challenge, an animal model for periodontitis will be developed and the sequential degeneration of tissues determined by studying their structure, chemical composition and mechanical properties. For the second challenge, tissue engineering (TE) can be used to create novel scaffolds. However, a major limitation of current TE procedures is limited knowledge of scaffold biomechanics. An efficient scaffold should sustain functional loads in addition to providing an environment conducive to desired cell behavior. To address these challenges, the inherent characteristics of the tissues and their interfaces must be determined. Then, they can be mimicked using TE to create appropriate scaffolds. Hence the following specific aims are defined to: 1). Investigate structure, chemical composition, mechanical properties of primary and secondary cementums, 2). Investigate structure, chemical composition, mechanical properties of cementum and its interface with root dentin, 3). Investigate strain fields using functional loads on anterior and posterior teeth as a function of age, 4). Perform a comparison studies between structure, chemical composition and mechanical properties of healthy human and rat alveolar bone, PDL, cementum, root dentin and their bimaterial interfaces. This information will be used to determine chronological changes in structure, chemical composition and mechanical properties in the rat periodontal tissues and their bimaterial interfaces during disease progression.

NIH独立奖奖K99/ROO应用的总体目的是提供 对指导阶段的支持年份，这对于完成四个假设的关键检验至关重要， 然后是手稿提交相关期刊。随后的3年独立阶段是 建立我的研究计划并实现我作为初级教师的目标所必需的。会 使我能够确立自己是生物材料整合和特征的独立研究者 考虑到人类和可比较的动物组织，随后建立井的组织 优化的生物工程接口。该奖项将使我能够研究所提出的特定目的定义 在此提案中，并扩展了我对建立下一代组织再生材料的知识。 这些努力的作用是基于一个普遍的假设，即牙齿的功能生物力学是 源自其在几个层次级别的不同组件的结构和化学相互作用 （宏观，微观和纳米尺度），这些产量依赖于位置，年龄和性别的产量。 包括牙周韧带（PDL），骨骼和骨骼在内的组织的破坏可能导致 由于牙周炎而引起的牙齿。主要挑战包括1）。了解相关组织的降解 随着疾病的进展和2）。将口腔组织结合在一起的界面的再生。到 应对第一个挑战，将开发出牙周炎的动物模型，然后进行顺序 通过研究其结构，化学成分和机械的结构来确定的组织变性 特性。对于第二个挑战，组织工程（TE）可用于创建新颖的脚手架。 但是，当前TE程序的主要局限性是对脚手架生物力学的了解有限。一个 有效的脚手架除了提供有利于所需的环境外，还应承担功能负载 细胞行为。为了应对这些挑战，组织及其界面的固有特征 必须确定。然后，可以使用TE模仿它们来创建适当的脚手架。因此 以下特定目标定义为：1）。研究结构，化学组成，机械 原发性和次级胶质的特性，2）。研究结构，化学组成， 牙骨质的机械性能及其与根牙本质的界面3）。使用 在前牙齿和后牙的功能载荷作为年龄的功能，为4）。比较研究 在结构，健康人和大鼠肺泡骨的化学成分和机械性能之间， PDL，胶质，根牙本质及其双层界面。此信息将用于确定 大鼠牙周的结构，化学成分和机械性能的时间顺序变化 疾病进展过程中的组织及其双层界面。