MACROMOLECULAR STRUCTURE DETERMINATION - GENERAL USERS

大分子结构测定 - 普通用户

• 批准号: 8363359
• 负责人: ROBERT M SWEET
• 金额: $ 130.89万
• 依托单位: BROOKHAVEN SCIENCE ASSOC-BROOKHAVEN LAB
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-01 至 2012-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363359
• 关键词: 3-Dimensional; ATP phosphohydrolase; Adenine; Alphavirus; Amyloid beta-Protein; Aspartate; Bacillus (bacterium); Bacterial RNA; Bacteriophage N4; Binding; Binding Proteins; Bypass; COPII-Coated Vesicles; CYP2B4 gene; Carbamyl Phosphate; Chromatin Remodeling Factor; Coenzyme A; Collection; Complex; Crystallography; DNA; DNA Repair Enzymes; DNA biosynthesis; DNA-Directed DNA Polymerase; DNA-Directed RNA Polymerase; DR1 gene; Data; Data Collection; Development; Elements; Entamoeba histolytica; Epidermal Growth Factor Receptor; Fatty Acid Desaturases; Fibrinogen; Flavivirus; Funding; Gene Silencing; Generations; Grant; Growth; Guanosine Triphosphate Phosphohydrolases; H-Meromyosin; HIV; HIV-1; HLA-DR4 Antigen; Histidine-Specific tRNA; Histocompatibility Antigens Class II; Human; Human Resources; Immune; Insulin; Laws; Lesion; Ligands; Light; MADD gene; Membrane Proteins; Micrococcal Nuclease; Mismatch Repair; Molecular Structure; Muramidase; National Center for Research Resources; O-GlcNAc transferase; Peptide Hydrolases; Peptides; Phosphoglucomutase; Porphobilinogen Synthase; Principal Investigator; Prolactin Receptor; Protein Synthesis Inhibitors; Proteins; Protons; RIPK2 gene; RNA; Research; Research Infrastructure; Research Personnel; Resolution; Resources; Ricinus communis; Roentgen Rays; Signal Transduction; Source; Spectrum Analysis; Structure; Synchrotrons; T-Cell Receptor; United States National Institutes of Health; abstracting; analog; anti-cancer therapeutic; base; cost; desaturase; design; env Gene Products; helicase; human BCAR1 protein; inhibitor/antagonist; kinase inhibitor; microbial; mutant; novel; protein complex; psychostimulant; receptor binding; research study; sensor; tRNA guanylyltransferase; triabin

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This subproject consists of a group of projects for which either abstracts were not available, or the total use was less than 0.1% of grant dollars, carried out by investigators who each consumed beamtime for the collection of x-ray diffraction data for 3-dimensional crystal structure solving. The investigators are included in the list of personnel, and they are the ones not identified with any other subproject. The investigators/subprojects for which their total use was less than 0.1% of grant dollars include the following: Thomas Steitz Structural Studies of tRNA-his Guanylyltransferase Stefan Riedl SHEP1/p130cas complex Deena Oren Fibrinogen/Abeta Victoria Robinson Structural Description of Novel Bacterial GTPases Gregory Bowman Crystal structure of human DDX3X helicase with ATP analogs Lucy Waskell Structure and spectroscopy of F429H mutant of cytochrome p450 2B4 Carolyn Cohen Heavy meromyosin Janice Pata Lesion-bypass DNA polymerases. Alex Bohm Mcv T-ag complex with DNA Prasanna Kolatkar Structure of Pax6 on non-canonical DNA elements Rudresh Acharya Crystal structure determination of M2 proton channel Yi Lu Lysozyme + DNA Huilin Li Outer membrane protein usher Ursula Ramirez Porphobilinogen synthase Yili Li HLA-DR4 complex with GP100 peptide Jurgen Sygusch The adenine riboswitch Victor Khangulov Internal Ionizable Residues in Staphylococcal nuclease Christopher Law Binding of dopaminergic psychostimulant to LeuT Yorgo Modis Flavivirus envelope protein and complexes John Shanklin Ivy Desaturase construct 2 Thomas Steitz Structural Studies of the 50S ribosomal subunit with substrate analogues Yorgo Modis Microbial ligand recognition by innate immune sensors Alba Guarne Cabello Studies of ADEPA with mutH Evan Kantrowitz Structure of Aspartate Trancarbamoylase from Bacillis subtilis with the substrate carbamoyl phosphate Karin Reinisch DENN-1 in complex with Rab35 Matthew Cordes Triabin-like Lipocalin 4 Edward Arnold Structural studies of HIV-1 RT and RNAP Byung ha Oh ST_Cas7 Yili Li Complex structure of CD4 and MHC class II molecule DR1 Liang Tang Structural studies of bacterial signal-transduction protein complexes Piotr Sliz Structure determination of human O-GlcNAc transferase and its complexes with peptides and inhibitors. Janice Pata Error-prone DNA synthesis Susan Buchanan Using the structure of MtmOIV for development of anti-cancer therapeutics Yong Xiong HIV factors and cellular interacting protein partners Ursula Ramirez Porphobilinogen synthase Christopher Hill Structural studies of Rpn13/Rpn2/Uch37complexes James Parsons PabB Karen Allen Beta-phosphoglucomutase Huei-Jiun Li 1,4-dihydroxy-2-naphthoyl-CoA synthase (MenB) in complex with inhibitors Jonathan Goldberg Structural Study of the Outer Shell of the COPII Vesicle Coat Alexei Soares Multi crystal experiments with insulin Bryan Nixon Mechanism of force generation by the AAA+ ATPase NtrC1 Seth Darst Bacterial RNA polymerase paused elongation complex Gregory Verdine Bacillus caldotenax UvrB (DNA Repair enzyme) Luis Brieba De Castro Structure of Inbitor of cistein Protease 1 from Entamoeba histolytica (EhICP1) Zhaocai Zhou EGFR kinase inhibitors Barbara Gerratana SibS Leemor Joshua-Tor Characterization of protein complexes involved in RNA induced transcriptional gene silencing Christopher Hill Partial Chromatin Remodeling Complex Jessica Bell FhbB MAD data collection Hongmin Li RIP2 binding protein Elias Lolis Structural basis for pH-dependent human Prolactin receptor recognition Kannan Natarajan Structures of T cell receptors, unliganded and in complex with MHC molecules John Shanklin Castor Bean wild-type fatty-acid desaturase Alba Guarne Cabello DNA Mismatch repair Mridhu Indic X-Ray Structure of FadL Mutants Yili Li NKp30 and Its ligand B7-H6 Elias Lolis MIF Enzymatic Inhibition and Mechanisms Andrew VanDemark DShrm SD2 domain Joseph Ippolito High Resolution Studies of the Large Ribosomal Subunit Bound to Newly Designed Inhibitors of Protein Synthesis Karen Allen Light chain inhibitor design Joseph Marcotrigiano Alphavirus replication proteins Katsuhiko Murakami Structure of bacteriophage N4 RNA polymerase2 and DNA complex Stevan Hubbard Growth Receptor Binding protein 14 RAPH - Ras Complex

该子项目是利用 NIH/NCRR 资助的中心拨款提供的资源的众多研究子项目之一。对子项目和子项目主要研究者的主要支持可能是由其他来源提供的，包括其他 NIH 来源。 子项目列出的总成本可能代表子项目使用的中心基础设施的估计金额，而不是 NCRR 拨款向子项目或子项目工作人员提供的直接资金。 该子项目由一组项目组成，这些项目要么没有摘要，要么总使用量低于拨款金额的 0.1%，由研究人员执行，每个研究人员都消耗光束时间来收集用于 3 维晶体结构求解的 X 射线衍射数据。 调查人员包含在人员名单中，并且他们是不属于任何其他子项目的人员。 总使用量低于拨款金额 0.1% 的研究者/子项目包括： Thomas Steitz tRNA-his 脒基转移酶的结构研究 Stefan Riedl SHEP1/p130cas 复合物 Deena Oren 纤维蛋白原/Abeta Victoria Robinson 新型细菌 GTP 酶的结构描述 Gregory Bowman 具有 ATP 类似物的人 DDX3X 解旋酶的晶体结构 Lucy Waskell 细胞色素 p450 2B4 F429H 突变体的结构和光谱 卡罗琳·科恩 重粒肌球蛋白 Janice Pata 病变绕过 DNA 聚合酶。 Alex Bohm Mcv T-ag 与 DNA 复合物 Prasanna Kolatkar 非规范 DNA 元件上 Pax6 的结构 Rudresh Acharya M2 质子通道晶体结构测定 易禄溶菌酶+DNA 李慧琳 外膜蛋白迎来 乌苏拉·拉米雷斯胆色素原合酶 伊利李 HLA-DR4 与 GP100 肽复合物 Jurgen Sygusch 腺嘌呤核糖开关 Victor Khangulov 葡萄球菌核酸酶中的内部电离残基 Christopher Law 多巴胺能精神兴奋剂与 LeuT 的结合 Yorgo Modis 黄病毒包膜蛋白和复合物 John Shanklin Ivy 去饱和酶构建体 2 Thomas Steitz 用底物类似物进行 50S 核糖体亚基的结构研究 Yorgo Modis 通过先天免疫传感器识别微生物配体 Alba Guarne Cabello 对 ADEPA 与 mutH 的研究 埃文·坎特罗维茨 (Evan Kantrowitz) 枯草芽孢杆菌天冬氨酸转氨甲酰酶的结构，底物为氨基甲酰磷酸 Karin Reinisch DENN-1 与 Rab35 的复合物 Matthew Cordes Triabin 样脂质运载蛋白 4 Edward Arnold HIV-1 RT 和 RNAP 的结构研究 吴秉河 ST_Cas7 CD4与MHC II类分子DR1的复合物结构 唐亮 细菌信号转导蛋白复合物的结构研究 Piotr Sliz 人 O-GlcNAc 转移酶及其与肽和抑制剂的复合物的结构测定。 Janice Pata 容易出错的 DNA 合成 Susan Buchanan 利用 MtmOIV 结构开发抗癌疗法 熊勇 HIV 因子和细胞相互作用蛋白伙伴 乌苏拉·拉米雷斯胆色素原合酶 Christopher Hill Rpn13/Rpn2/Uch37 复合物的结构研究 詹姆斯·帕森斯 PabB 凯伦·艾伦 β-磷酸葡萄糖变位酶 Huei-Jiun Li 1,4-二羟基-2-萘酰辅酶A合酶（MenB）与抑制剂的复合物 乔纳森·戈德堡 (Jonathan Goldberg) COPII 囊泡涂层外壳的结构研究 Alexei Soares 胰岛素多晶体实验 布莱恩·尼克松 AAA+ ATPase NtrC1 产生力的机制 Seth Darst 细菌 RNA 聚合酶暂停延伸复合物 Gregory Verdine 热坚芽孢杆菌 UvrB（DNA 修复酶） Luis Brieba De Castro 溶组织内阿米巴 (EhICP1) 半​​胱氨酸蛋白酶 1 抑制剂的结构 周兆才 EGFR 激酶抑制剂 芭芭拉·格拉塔娜·西伯斯 Leemor Joshua-Tor 参与 RNA 诱导转录基因沉默的蛋白质复合物的表征 Christopher Hill 部分染色质重塑复合物 杰西卡·贝尔 FhbB MAD 数据采集 李红民 RIP2 结合蛋白 Elias Lolis pH 依赖性人类催乳素受体识别的结构基础 Kannan Natarajan T 细胞受体的结构，未配体且与 MHC 分子复合 约翰·尚克林蓖麻子野生型脂肪酸去饱和酶 Alba Guarne Cabello DNA 错配修复 FadL 突变体的 Mridhu Indic X 射线结构 李伊力 NKp30 及其配体 B7-H6 Elias Lolis MIF 酶抑制和机制 安德鲁·范德马克 DShrm SD2 域 Joseph Ippolito 对与新设计的蛋白质合成抑制剂结合的大核糖体亚基的高分辨率研究 Karen Allen 轻链抑制剂设计 Joseph Marcotrigiano 甲病毒复制蛋白 噬菌体 N4 RNA 聚合酶 2 和 DNA 复合物的结构 Stevan Hubbard 生长受体结合蛋白 14 RAPH - Ras 复合物