RAPIDATA AND OTHER THINGS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 在国家同步加速器光源(NSLS)举办的大分子结晶学方法国际上最广泛认可的课程之一。自1999年以来，每年春天，来自世界各地的学生都会聚集在NSLS参加RapiData，这是一个由Sweet、Alex Soares和Sal Sclafani组织的为期一周的速成课程，是P41项目的培训部分。它的目的是向参与者介绍最好的和最新的大分子X射线结晶学设备和技术。RapiData允许学生与领先的软件开发人员见面并向他们学习 在结晶学领域，然后实际使用NSLS光束线收集数据。今年，52名学生参加了4月4日至9日举行的研讨会。 自开设以来，已有近650名学生参加了这门课程，我们不断要求增加更多的名额。许多RapiData校友现在已经成长为结晶学专家，他们继续将经验较少的同事推荐到BNL，以了解这一高度专业化的感兴趣领域的初始步骤。我们经常收到以前RapiData学生的研究生。 课程从Sweet教授的为期一天的背景课程开始，旨在为经验较少的学生更新晶体基础知识。接下来的两天充满了来自BNL、工业、学术界和其他国家实验室的科学家的讲座和教程。接下来，学生们被分组，并在8条NSLS光束线上引导进行了长达60小时的马拉松式数据收集课程。与此同时，许多教程正在进行中，由讲师自己管理。近三分之二的学生带来了自己的标本，目的是解开特定酶的结构，而其他人则观察并帮助他们。在课程期间，大约解决了六个结构，每个结构都可能是一个可发布的结果。在课程的数据收集部分，学生们每天下午聚集在一起讨论他们的结果，并制作关于他们的数据和科学主题的小型讲座。 看着学生们整个星期的技能发展总是令人兴奋的。他们中的许多人来到这里时几乎没有结晶学经验，离开时他们知道如何获取和处理真实数据，以及如何在出现问题时定位和修复问题。这就是拥有这样一个动手项目的优势。 这笔赠款为课程本身提供了支持，教育、环境和社会保障部提供了实物资助，并对当地工作人员和光束线业务提供了部分支持。布鲁克海文科学协会、NSLS以及几家设备供应商和制药公司提供了额外的支持。此外，国际结晶学联合会还提供了一笔特别补助金，帮助七名拉丁美洲学生参加这门课程。 此外，当然，当我们的科学家和PX操作员与我们光束线的外部用户互动时，获取和处理好的衍射数据所需的技能培训是一个持续的过程。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. One of the most widely recognized courses internationally in macromolecular crystallography methods is held at the National Synchrotron Light Source (NSLS). Since 1999, students from around the world have gathered at the NSLS every spring for RapiData, a weeklong crash course organized by Sweet, Alex Soares, and Sal Sclafani, a training component of this P41 project. It's designed to introduce participants to the best and latest equipment and techniques for macromolecular x-ray crystallography. RapiData allows students to meet and learn from the leading developers of software in the crystallography field, and then to actually use NSLS beamlines to collect data. This year, 52 students attended the workshop, which was held from April 4 - 9. Since its creation, nearly 650 students have attended this course, and we have constant requests to add more slots. Many RapiData alumni have now blossomed into crystallography experts, and they continue to refer their less-experienced colleagues to BNL to learn the initial steps of this highly specialized area of interest. We regularly get graduate students of former RapiData students. The course began with a daylong background session taught by Sweet, meant to refresh the knowledge of crystallography fundamentals for less-experienced students. The following two days were filled with lectures and tutorials taught by scientists from BNL, industry, academia, and other national labs. Next, the students were divided into groups and guided through a marathon, 60-hour data-collection session on eight NSLS beamlines. At the same time, many tutorials were underway, run by the lecturers themselves. Nearly two-thirds of the students brought their own specimens with the goal of solving the structure of a particular enzyme, while the others observed and helped. About half a dozen structures were solved during the course, each of which could be a publishable result. During the data-collection portion of the course, the students gathered each afternoon to discuss their results and produced mini lectures about their data and scientific subject. It's always really exciting to watch the students' skills develop throughout the week. Many of them come here with little or no experience in crystallography, and they leave knowing how to obtain and process real data, as well as how to locate and fix problems as they arise. That's the advantage of having such a hands-on program. There was support from this grant for the course itself, and BER/DOE provided in-kind funding with their partial support of local staff and beamline operations. Additional support was provided by Brookhaven Science Associates, the NSLS, and several equipment vendors and drug companies. In addition a special grant was provided by the International Union of Crystallography to assist seven Latin American students in attending the course. In addition, of course, training in the skills required to take and process good diffraction data are a continuous process as our scientists and PX Operators interact with outside users of our beamlines.