MEASUREMENT OF GLUCOSE HOMEOSTASIS IN HUMAN BRAIN BY NMR

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 (in与医学博士Elizabeth Seaquist合作，由R 01 NS 035192，PI Seaquist支持）：该项目的总体目标是确定糖尿病的影响和强化治疗对人体体内脑葡萄糖代谢的低血糖后果。在这个资助周期中，我们通过13 C-葡萄糖输注结合13 C-MR光谱研究了人脑中的糖原代谢。 我们首先测定了正常和轻度高血糖时健康志愿者的糖原含量和转换率。 然后我们研究了低血糖时脑糖原的作用。 我们假设人类大脑在低血糖期间利用糖原，并且在低血糖后使其超过正常水平（“超补偿”），如在啮齿动物中所示。 了解低血糖期间和低血糖后糖原在人脑中的作用是重要的，因为糖原超代偿可能涉及低血糖无意识，这是糖尿病患者胰岛素治疗的主要障碍。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. (in collaboration with Dr. Elizabeth Seaquist, Medicine, supported by R01 NS035192, PI Seaquist): The overall goal of this project is to determine the effects of diabetes and the hypoglycemic consequences of intensive therapy on in vivo brain glucose metabolism in humans. In this grant cycle, we study glycogen metabolism in the human brain by 13C MR spectroscopy in conjunction with infusions of 13C-glucose. We first determined the glycogen content and turnover rate in healthy volunteers during eu- and slight hyperglycemia. We then investigated the role of brain glycogen during hypoglycemia. We hypothesized that the human brain utilizes glycogen during hypoglycemia and overshoots it to above normal levels ("supercompensates") following hypoglycemia, as was shown to occur in rodents. Understanding the role of glycogen in the human brain during and following hypoglycemia is important because glycogen supercompensation may be involved in hypoglycemia unawareness which is a major barrier to insulin treatment in patients with diabetes.