BRAIN GLUCOSE TRANSPORT IN SUBJECTS & PATIENTS WITH DIABETES FOLLOWING HYPOGLYC

该子项目是利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得主要资金， 因此可以在其他CRISP条目中表示。列出的机构是 中心，不一定是研究者的机构。 虽然已经确定复发性低血糖会导致低血糖无意识，但其机制尚不清楚。一种假设是，反复发生的低血糖改变了大脑葡萄糖转运或代谢。我们在葡萄糖钳夹期间测量稳态脑葡萄糖浓度，以确定1型糖尿病和低血糖无意识受试者在暴露于复发性低血糖后是否可能改变脑葡萄糖转运或代谢。我们比较了14名糖尿病和低血糖无意识的受试者和27名健康对照受试者。在类似的代谢条件下，在使用生长抑素和胰岛素的高血糖钳夹（血浆葡萄糖= 16.7 mmol/l）期间，使用体内1H核磁共振（NMR）光谱在4特斯拉下测量脑葡萄糖浓度。在相同条件下，与对照组相比，1型糖尿病和低血糖无意识受试者的脑葡萄糖浓度显著较高（5.5 <$0.3 vs 4.7 <$0.1 mol/g湿重，P = 0.016）。这些数据表明，脑葡萄糖转运或代谢的变化可能是反复低血糖的结果

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Although it is well established that recurrent hypoglycemia leads to hypoglycemia unawareness, the mechanisms responsible for this are unknown. One hypothesis is that recurrent hypoglycemia alters brain glucose transport or metabolism. We measured steady-state brain glucose concentrations during a glucose clamp to determine whether subjects with type 1 diabetes and hypoglycemia unawareness may have altered cerebral glucose transport or metabolism after exposure to recurrent hypoglycemia. We compared 14 subjects with diabetes and hypoglycemia unawareness to 27 healthy control subjects. Brain glucose concentrations were measured under similar metabolic conditions using in vivo 1H nuclear magnetic resonance (NMR) spectroscopy at 4 Tesla during a hyperglycemic clamp (plasma glucose = 16.7 mmol/l) with somatostatin and insulin. Subjects with type 1 diabetes and hypoglycemia unawareness had significantly higher brain glucose concentrations compared to that in controls under the same conditions (5.5 ¿ 0.3 vs. 4.7 ¿ 0.1 mol/g wet weight, P = 0.016). These data suggest that changes in brain glucose transport or metabolism may occur as a result of recurrent hypoglycemia