PORCINE REPRODUCTIVE & RESPIRATORY SYNDROME VIRUS-ASSOCIATED GLYCANS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 猪繁殖与呼吸综合征病毒(PRRSV)是猪繁殖与呼吸综合征的病原，是世界养猪业面临的最严重的传染病。PRRSV是高度宿主限制性的病毒，在猪巨噬细胞中优先生长。在体外，PRRSV在猪肺泡巨噬细胞的原代培养中生长，以及在MARC-145细胞中生长，MARC-145细胞是非洲绿猴肾上皮样细胞系，通常用于PRRSV野生型和疫苗株的体外繁殖。然而，在这两种宿主细胞之间，某种PRRSV毒株通常对其中一种细胞类型比另一种细胞类型更有偏好。了解PRRSV的分子结构有望促进细胞嗜性的研究和疾病预防策略的发展。PRRSV有一个光滑的球形包膜，包裹着主要的包膜蛋白(GP5/M异二聚体)和次要的包膜蛋白(E、GP2a、GP3、GP4)，其中大多数是高度糖基化的。GP5连接的多聚糖的相对分子质量超过了它的短胞外域氨基酸序列。因此，广泛分布的病毒多聚糖可能覆盖病毒粒子表面，甚至延伸为触角，这表明在病毒与细胞相互作用中可能发挥作用，并可能成为杀微生物剂设计中病毒中和的目标。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Porcine reproductive and respiratory syndrome virus (PRRSV) is the etiologic agent of porcine reproductive and respiratory syndrome, which is the most severe infectious disease facing the swine industry worldwide. PRRSV is highly host-restricted, growing preferentially in vivo in porcine macrophages. In vitro, PRRSV grows in primary cultures of porcine alveolar macrophages, as well as in MARC-145 cells, the African green monkey kidney epithelial-like cell line, which have been routinely used for in vitro propagation of wild-type and vaccine strains of PRRSV. However, between the two types of host cells, a certain PRRSV strain usually has a preference on one cell type over the other. Understanding the molecular structure of PRRSV is expected to facilitate the study of cell tropism and the development of disease prevention strategies. PRRSV has a smooth spherical envelope embedded with major envelope proteins (GP5/M heterodimer) and minor envelope proteins (E, GP2a, GP3, GP4), most of which are highly glycosylated. The molecular weight of GP5-linked glycans exceeds that of its short ectodomain amino acid sequences. Thus the broadly distributed viral glycans are likely to cover the virion surface, or even stretch out as antennae, suggesting a potential role in virus-cell interactions and possible targets for virus neutralization in microbicide design.