PORCINE REPRODUCTIVE & RESPIRATORY SYNDROME VIRUS-ASSOCIATED GLYCANS

猪繁殖

• 批准号: 8363119
• 负责人: RON ORLANDO
• 金额: $ 0.34万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363119
• 关键词: Alveolar Macrophages; Amino Acid Sequence; CCL7 gene; CD36 gene; Cell Line; Cells; Cercopithecus pygerythrus; Communicable Diseases; Development; Epithelial; Family suidae; Funding; Grant; In Vitro; Industry; Kidney; Link; Minor; Molecular Structure; Molecular Weight; National Center for Research Resources; Polysaccharides; Porcine Reproductive and Respiratory Syndrome; Porcine respiratory and reproductive syndrome virus; Prevention strategy; Principal Investigator; Research; Research Infrastructure; Resources; Role; Source; Stretching; Surface; Technology; Tropism; United States National Institutes of Health; Vaccines; Viral; Virion; Virus; Virus-Cell Membrane Interaction; cell type; cost; design; disorder prevention; env Gene Products; in vivo; macrophage; microbicide; preference; protein E

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Porcine reproductive and respiratory syndrome virus (PRRSV) is the etiologic agent of porcine reproductive and respiratory syndrome, which is the most severe infectious disease facing the swine industry worldwide. PRRSV is highly host-restricted, growing preferentially in vivo in porcine macrophages. In vitro, PRRSV grows in primary cultures of porcine alveolar macrophages, as well as in MARC-145 cells, the African green monkey kidney epithelial-like cell line, which have been routinely used for in vitro propagation of wild-type and vaccine strains of PRRSV. However, between the two types of host cells, a certain PRRSV strain usually has a preference on one cell type over the other. Understanding the molecular structure of PRRSV is expected to facilitate the study of cell tropism and the development of disease prevention strategies. PRRSV has a smooth spherical envelope embedded with major envelope proteins (GP5/M heterodimer) and minor envelope proteins (E, GP2a, GP3, GP4), most of which are highly glycosylated. The molecular weight of GP5-linked glycans exceeds that of its short ectodomain amino acid sequences. Thus the broadly distributed viral glycans are likely to cover the virion surface, or even stretch out as antennae, suggesting a potential role in virus-cell interactions and possible targets for virus neutralization in microbicide design.

该副本是利用资源的众多研究子项目之一 由NIH/NCRR资助的中心赠款提供。对该子弹的主要支持 而且，副投影的主要研究员可能是其他来源提供的 包括其他NIH来源。 列出的总费用可能 代表subproject使用的中心基础架构的估计量， NCRR赠款不直接向子弹或副本人员提供的直接资金。 猪繁殖和呼吸道综合征病毒（PRRSV）是猪生殖和呼吸综合征的病因学，这是全球面临的最严重的传染病。 PRRSV高度受宿主的限制性，在猪巨噬细胞中优先生长在体内。在体外，PRRSV在猪肺泡巨噬细胞的原发性培养物以及MARC-145细胞中生长，非洲绿色猴子肾肾上皮细胞系通常用于体外传播PRRSV的野生型和疫苗菌株的体外传播。但是，在两种类型的宿主细胞之间，特定的PRRSV菌株通常对一种细胞类型比另一种细胞类型更偏爱。理解PRRSV的分子结构有望促进细胞卓越的研究和疾病预防策略的发展。 PRRSV具有嵌入主要包膜蛋白（GP5/M异二聚体）和次要包膜蛋白（E，GP2A，GP3，GP4）的光滑球形包膜，其中大多数是高度糖基化的。 GP5连接的聚糖的分子量超过了其短域氨基酸序列的分子量。因此，广泛分布的病毒甘氨酸可能覆盖病毒体表面，甚至延伸为触角，这表明在病毒 - 细胞相互作用中具有潜在的作用，并且可能在杀菌剂设计中中和病毒中和。