GLYCOPROTEIN GLYCOMICS: EXPRESSION IN MOUSE ES CELLS & PROGENY

糖蛋白糖组学：小鼠 ES 细胞中的表达

• 批准号: 8363005
• 负责人: RON ORLANDO
• 金额: $ 6.02万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363005
• 关键词: Affinity Chromatography; Bioinformatics; Cataloging; Catalogs; Cells; Data; Embryo; Fractionation; Funding; Glycopeptides; Glycoproteins; Grant; Lectin; Link; Liquid Chromatography; Mass Spectrum Analysis; Mus; National Center for Research Resources; Peptide N-Glycosidase; Peptides; Polysaccharides; Principal Investigator; Research; Research Infrastructure; Resources; Source; Staging; Stem cells; Technology; United States National Institutes of Health; cost; embryonic stem cell; liquid chromatography mass spectrometry; tool

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Core 2A is developing tools to analyze the Glycoprotein Glycome of cells, including embryonic mouse stem cells and their differentiated products. Fractionation of tryptic glycopeptides using serial lectin affinity chromatography is being developed to classify expression by the types of glycans expressed. Separation of glycopeptides is then further accomplished by liquid chromatography, followed by mass spectrometry to catalog and identify various species. At various stages, N-linked glycans will be produced by N-glycanase treatment. The resulting peptides will be identified by their Asn to Asp conversion using LC/MS analysis. All results will be submitted to the Bioinformatics module (Core project 4) for integration with other data.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 Core 2A 正在开发分析细胞糖蛋白糖组的工具，包括小鼠胚胎干细胞及其分化产物。正在开发使用系列凝集素亲和层析对胰蛋白酶糖肽进行分级，以根据表达的聚糖类型对表达进行分类。然后通过液相色谱法进一步完成糖肽的分离，然后通过质谱法来分类和鉴定各种物种。在各个阶段，N-连接聚糖将通过N-聚糖酶处理产生。所得肽将通过 LC/MS 分析将 Asn 转化为 Asp 进行鉴定。所有结果将提交给生物信息学模块（核心项目4）以与其他数据集成。