DEVELOPMENT OF TECHNIQUES FOR QUANTITATIVE GLYCOMICS

定量糖组学技术的发展

• 批准号: 8363021
• 负责人: RON ORLANDO
• 金额: $ 3.44万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363021
• 关键词: Affinity; Cations; Complex Mixtures; Funding; Grant; Individual; Mass Spectrum Analysis; Methodology; Methods; Molecular; National Center for Research Resources; Polysaccharides; Principal Investigator; Protons; Research; Research Infrastructure; Resources; Source; Structure; Surface; Technology; United States National Institutes of Health; comparative; cost; improved; ionization; molecular mass; research study; technique development

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The quantitative comparison of glycan expression requires highly sensitive methods that can distinguish and identify individual glycans with subtly different structures. Mass spectrometry (MS) fulfills these requirements while providing a rapid and reliable method to analyze complex mixtures, and therefore has been widely used in glycomic studies. One limitation of using MS for the quantitation of biomolecules is that the ionization efficiencies of distinct molecular species can differ significantly, depending on such factors as the analyte's molecular mass, proton/cation affinity, surface activity, the presence of compounds which compete with or interfere with the ionization of the analyte, etc. We are involved in devising new methodologies to improve the accuracy of comparative glycomic experiments.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 聚糖表达的定量比较需要高灵敏度的方法，这些方法可以区分和鉴定具有细微不同结构的单个聚糖。 质谱法（MS）满足了这些要求，同时提供了一种快速可靠的方法来分析复杂的混合物，因此已被广泛用于糖组学研究。 使用MS定量生物分子的一个限制是不同分子种类的电离效率可能显著不同，这取决于诸如分析物的分子质量、质子/阳离子亲和力、表面活性、与分析物的电离竞争或干扰分析物的电离的化合物的存在、我们参与设计新的方法，以提高比较糖组学实验的准确性。