COMPARATIVE PROTEOMICS AND GLYCOPROTEOMICS OF PROTEINS SECRETED FROM B CINEREA

灰霉病菌分泌蛋白质的比较蛋白质组学和糖蛋白质组学

• 批准号: 8363013
• 负责人: RON ORLANDO
• 金额: $ 6.02万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363013
• 关键词: Affect; Botrytis; Cell Wall; Development; Economics; Enzymes; Flowers; Funding; Grant; Gray unit of radiation dose; Growth; Harvest; Infection; Molds; National Center for Research Resources; Phase; Plants; Prevention strategy; Principal Investigator; Process; Proteins; Proteome; Proteomics; Recombinant Proteins; Research; Research Infrastructure; Resources; Source; Technology; Tissues; Trees; United States National Institutes of Health; comparative; cost; fruits and vegetables; fungus; glycosylation; pathogen; protein structure

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The fungus Botrytis cinerea is a widespread plant pathogen that affects many vegetables and fruits, as well as a large number of shrubs, trees, flowers, and weeds by causing grey mold or soft rot on the host tissues. B. cinerea mainly infects dicotyledons and non-graminaceous monocotyledons, and the infection not only occurs during the growth phase of crops but also during storage of the harvested products. Thus, it is responsible for significant economic losses. A great deal of research has focused on understanding the B. cinerea infection process, which in turn directs the development of effective prevention strategies. B. cinerea must breach the cell wall during the infection process and accordingly secretes a great number of enzymes that can degrade cell walls. Little is known concerning the identity of these enzymes. The majority of these cell wall degrading enzymes are glycosylated. Although the glycosylation on several recombinant proteins have been characterized, very little is known on the native enzymes, despite the importance of glycosylation on protein structure and activity. To this end, we have started to characterize the secreted proteome and glycoproteome of this fungus.

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 灰葡萄孢（Botrytis cinerea）是一种广泛分布的植物病原体，通过在宿主组织上引起灰霉病或软腐病而影响许多蔬菜和水果以及大量灌木、树木、花卉和杂草。B。灰霉病主要侵染双子叶植物和非禾本科单子叶植物，不仅发生在作物的生长期，而且发生在收获产品的储存期。因此，它造成了重大的经济损失。大量的研究集中在理解B上。灰霉病的感染过程，这反过来又指导了有效的预防策略的发展。B。灰霉病菌在感染过程中必须破坏细胞壁，并因此分泌大量可降解细胞壁的酶。关于这些酶的特性知之甚少。这些细胞壁降解酶大多数是糖基化的。虽然糖基化对几种重组蛋白质的特征，很少知道的天然酶，尽管糖基化对蛋白质的结构和活性的重要性。为此，我们已经开始表征这种真菌的分泌蛋白质组和糖蛋白质组。