PROTON NMR OF 15 OLIGOSACCHARIDES

15 种低聚糖的质子核磁共振

• 批准号: 8363089
• 负责人: Parastoo Azadi
• 金额: $ 0.17万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8363089
• 关键词: Acetone; COSY; Chemicals; Deuterium; Freeze Drying; Funding; Grant; Measures; Methods; NMR Spectroscopy; NOESY; National Center for Research Resources; Oligosaccharides; Principal Investigator; Protons; ROESY; Relative (related person); Research; Research Infrastructure; Resources; Running; Sampling; Source; TOCSY; Technology; Tube; United States National Institutes of Health; Water; cost

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Methods: NMR Spectroscopy The samples were deuterium exchanged by dissolving in D2O and lyophilization. The samples were dissolved in 80 ¿L D2O, 2 ¿L 1 % acetone in D2O was added as internal reference, and the samples were placed in a 3 mm Shigemi NMR tube. 1-D Proton, TOCSY and NOESY or ROESY NMR spectra, run with water presaturation, and gradient enhanced COSY and HSQC spectra were acquired on a Varian Inova-800 MHz spectrometer at 25 ¿C. Chemical shifts were measured relative to internal acetone (¿H=2.218 ppm, ¿C=33.00 ppm). Standard Varian acquisition parameters were used.

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 方法： 核磁共振波谱 样品经D2O溶解、冷冻干燥后进行换氢处理。样品用80°L D2O溶解，2°L加入1%丙酮在D2O中作为内标，样品放置在3 mm的Shigemi核磁共振管中。用水预饱和获得了一维质子、TOCSY和NOESY或ROESY核磁共振谱，并在25℃的Varian Inova-800 MHz光谱仪上获得了梯度增强的COSY和HSQC谱，测量了相对于内部丙酮的化学位移(H=2.218 ppm，C=33.00ppm)。使用标准的瓦里安采集参数。