MASS SPECTROMETRY OF GLYCOPROTEINS

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 这是一个为期两天的课程，由CCRC教员讲授，包括绘制糖蛋白中的糖基化位点图，确定N-和O-连接的寡糖的组成、测序和分支点，以及这些分析中使用的MS程序。在基质辅助激光解吸(MALDI)和电喷雾电离(ESI-MS)仪器上进行了演示。向每个课程参与者提供了一份课程手册，其中包括选定的分析技术和参考资料。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This is a two-day course with lectures by CCRC faculty and includes mapping the glycosylation sites in glycoproteins, determining the composition, sequencing, and branching points of N- and O-linked oligosaccharides, and MS procedures used in these analyses. Demonstrations are performed on both matrix-assisted laser desorption (MALDI) and electrospray ionization (ESI-MS) instruments. A course manual including selected analytical techniques and references is provided to each course participant.