A National Glycoscience Resource - CCRC Service and Training

国家糖科学资源 - CCRC 服务和培训

• 批准号: 10707084
• 负责人: Parastoo Azadi
• 金额: $ 74.71万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-18 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10707084
• 关键词: Area; Bacterial Polysaccharides; Biochemical; Biological Assay; Carbohydrates; Categories; Cell Differentiation process; Cells; Chemicals; Communities; Complex; Country; Disaccharides; Educational workshop; Fingerprint; Funding; GAG Gene; Gas Chromatography; Glycoconjugates; Glycolipids; Glycopeptides; Glycoproteins; Glycosaminoglycans; Glycosphingolipids; Goals; Home; Individual; Industry; Infrastructure; Maps; Mass Spectrum Analysis; Molecular; Molecular Weight; Monosaccharides; National Institute of General Medical Sciences; Nonprofit Organizations; Nuclear Magnetic Resonance; Output; Polysaccharides; Property; Protein-Carbohydrate Interaction; Protocols documentation; Reporting; Research; Research Personnel; Research Training; Resources; Sampling; Science; Scientist; Security; Services; Site; Structure; Techniques; Technology; Tissues; Training; Training Activity; United States National Institutes of Health; Universities; archive data; cell type; chemical synthesis; data archive; depolymerization; experience; genetic linkage analysis; glycoproteomics; glycosylation; instrument; interest; liquid chromatography mass spectrometry; meetings; molecular modeling; new technology; outreach; physical separation; stem; symposium; tool; transcriptome

PROJECT SUMMARY The goal of this proposal is to create a National Resource in Glycoscience that will offer service and hands- on training for compositional analysis and detailed structural characterization of all classes of glycoconjugates, including glycoproteins, polysaccharides, and glycolipids, to the greater scientific community. The Complex Carbohydrate Research Center (CCRC) at the University of Georgia has been home to two NIH/NIGMS federally funded resource centers: the National Center for Biomedical Glycomics (5P41GM103490), where new technologies in mass spectrometry and transcriptome analysis have been developed to investigate glycomics and glycoproteomics of stem and differentiated cell types; and the Research Resource for Integrated Glycotechnology (5P41GM103390), in which mass spectrometry, NMR, molecular modeling, chemical synthesis and cell based assays have been used to investigate the molecular basis of protein-carbohydrate interactions. The CCRC service facility has offered a variety of complementary services using technologies developed by both research resources that are now mature and completely translatable to investigating the glycomes and glycoproteomes of any tissue or cell type. Since the NIGMS Research Resources have recently been sunsetted, we propose to combine the “high demand” services from each resource and create a “National Glycoscience Resource”-CCRC Service and Training (GlyCore) that will enable us to continue to offer and enhance our cutting edge services in glycoscience, hands-on training courses and workshops and to make them easily accessible to the biomedical science community. GlyCore will offer a comprehensive list of services, including isolation and purification, as well as NMR and MS characterization of all glycoconjugates that are grouped into several categories and are integrated together to help solve particular problems in glycoscience. In order to better make our technologies available to outside investigators, we will also continue to offer four well- received and well-established hands-on training courses in glycoscience. Our experience has demonstrated that the one-on-one format of our training is extremely effective in achieving acceptance of tools in glycoscience. We have a combination of state-of-the-art NMR and MS instruments and well-trained scientists with particular expertise and track record for service and training in carbohydrate structural analysis. We have fully implemented and well-developed protocols for sample receipt, sample handling, sample tracking, storage, data archiving, and reporting that provide our users with security and analytic outputs of uniform quality. The number of research groups that have been impacted by our service and training activities spans the whole country and extend globally. We have built an easily accessible “go-to” service and training facility through extensive outreach activities, including our Glyco-booth at meetings, seminars, and workshops at conferences, and building close relationships with universities, scientific non-profit organizations, and industries.

项目概要 该提案的目标是创建一个糖科学国家资源，提供服务和实践 关于所有类别的成分分析和详细结构表征的培训 糖复合物，包括糖蛋白、多糖和糖脂，以更科学的方式 社区。佐治亚大学复杂碳水化合物研究中心 (CCRC) 拥有两个 NIH/NIGMS 联邦资助的资源中心：国家生物医学糖组学中心 （5P41GM103490），其中质谱和转录组分析的新技术已被采用 开发用于研究干细胞和分化细胞类型的糖组学和糖蛋白质组学；和研究 集成糖技术资源 (5P41GM103390)，其中质谱、核磁共振、分子 建模、化学合成和基于细胞的测定已被用来研究分子基础 蛋白质-碳水化合物相互作用。 CCRC 服务设施提供了多种补充服务 使用两种研究资源开发的技术，这些技术现在已经成熟并且完全可以转化为 研究任何组织或细胞类型的糖组和糖蛋白质组。自 NIGMS 研究资源 最近已经日落，我们建议将每种资源的“高需求”服务结合起来 创建“国家糖科学资源”-CCRC 服务和培训 (GlyCore)，这将使我们能够继续 提供和增强我们在糖科学、实践培训课程和研讨会方面的尖端服务 使生物医学科学界能够轻松获取它们。 GlyCore 将提供一份完整的清单 服务，包括分离和纯化，以及所有糖复合物的 NMR 和 MS 表征 分为几个类别并整合在一起以帮助解决糖科学中的特定问题。 为了更好地向外部研究人员提供我们的技术，我们还将继续提供四个良好的 已接受且完善的糖科学实践培训课程。我们的经验证明 我们的一对一培训形式对于糖科学工具的接受非常有效。我们 拥有最先进的 NMR 和 MS 仪器以及训练有素的科学家， 碳水化合物结构分析方面的专业知识和服务和培训记录。我们已全面落实 以及完善的样品接收、样品处理、样品跟踪、存储、数据归档和 为我们的用户提供安全性和统一质量的分析输出的报告。研究数量 受到我们服务和培训活动影响的群体遍布全国并延伸到 全球。我们通过广泛的外展活动建立了一个易于使用的“首选”服务和培训设施 活动，包括我们在会议、研讨会和研讨会上的 Glyco 展位，以及建立密切的联系 与大学、科学非营利组织和行业的关系。