NMR OF 6 SAMPLES

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 研究方法： NMR 通过冻干将样品（20 mg）从D2 O（99.9%D）中进行氘交换，溶解于0.7 mL D2 O（99.96%D）中，加入100 µ L 10 mg/mL DSS的D2 O（99.96%D）溶液，并将溶液置于5 mm NMR管中。 一维质子NMR光谱在Varian Inova-500 MHz仪器上在40 ℃下获得。 光谱宽度为4.7kHz，发射器频率设置为-587Hz，并且在每个实验中收集32次扫描。 使用Mestre-C软件使用指数乘法（0.2Hz）处理数据。 光谱参考最高场DSS信号（0.0 ppm）。用三阶伯恩斯坦多项式对光谱进行基线校正。 通过对5.97 ppm（<$UA 2S-H4）和5.79 ppm（<$UA-H4）处的信号进行积分，测定<$UA 2S-H4与<$UA-H4的比值。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Methods: NMR The samples (20 mg) were deuterium-exchanged by lyophilization from D2O (99.9 % D), dissolved in 0.7 mL D2O (99.96 % D), 100 ¿L 10 mg/mL DSS in D2O (99.96 % D) was added, and the solution placed into a 5-mm NMR tube. One-dimensional proton NMR spectra were acquired on a Varian Inova-500 MHz instrument at 40 ¿C. Spectral width was 4.7 kHz, transmitter frequency was set at -587 Hz, and 32 scans were collected in each experiment. The data were processed using the Mestre-C software using exponential multiplication (0.2 Hz). Spectra were referenced to the most upfield DSS signal (0.0 ppm). The spectra were baseline corrected with a third order Bernstein polynomial. The ratio of ¿UA2S-H4 to ¿UA-H4 was determined by integration of the signals at 5.97 ppm ( ¿UA2S-H4) and 5.79 ppm (¿UA-H4).