A National Glycoscience Resource - CCRC Service and Training

项目总结 这项提案的目标是创建一个国家血糖科学资源，提供服务和人手- 浅谈各班成分分析和详细结构表征的训练 糖偶联物，包括糖蛋白、多糖和糖脂，对更大的科学 社区。佐治亚大学的复合碳水化合物研究中心(CCRC)已经 NIH/NIGMS联邦资助的两个资源中心的所在地：国家生物医学血糖中心 (5P41GM103490)，其中质谱学和转录组分析方面的新技术 旨在研究干细胞和分化细胞类型的糖组学和糖蛋白组学； 集成糖技术资源(5P41GM103390)，其中包括质谱学、核磁共振、分子 已使用建模、化学合成和基于细胞的分析来研究其分子基础 蛋白质-碳水化合物的相互作用。中国证监会的服务设施提供了多种补充服务 使用由两个研究资源开发的技术，这些资源现在已经成熟，完全可以翻译成 研究任何组织或细胞类型的糖类和糖蛋白。自从NIGMS研究资源 ，我们建议将每个资源的“高需求”服务和 创建“国家糖学资源”--中国中车服务与培训(GlyCore)，这将使我们能够继续 提供和加强我们在糖科学、实践培训课程和研讨会方面的尖端服务 让生物医学科学界很容易接触到它们。GlyCore将提供一份全面的清单 服务，包括分离和纯化，以及所有糖结合物的核磁共振和MS表征 被分成几个类别，并被整合在一起，以帮助解决糖科学中的特定问题。 为了更好地向外部调查人员提供我们的技术，我们还将继续提供四口井- 广受好评且行之有效的糖科学实践培训课程。我们的经验表明， 我们一对一培训的形式在实现对血糖科学工具的接受方面非常有效。我们 拥有最先进的核磁共振和MS仪器和训练有素的科学家，特别是 在碳水化合物结构分析服务和培训方面的专业知识和记录。我们已经全面实施了 和完善的样品接收、样品处理、样品跟踪、存储、数据存档和 为我们的用户提供统一质量的安全和分析输出的报告。研究的数量 受我们服务和培训活动影响的群体遍及全国各地，并不断扩大 全球范围内。我们通过广泛的外展，建立了方便快捷的“首选”服务和培训设施 活动，包括我们在会议、研讨会和研讨会上的Glyco展台，以及近距离的建筑 与大学、科学非营利组织和行业的关系。

PROJECT SUMMARY The goal of this proposal is to create a National Resource in Glycoscience that will offer service and hands- on training for compositional analysis and detailed structural characterization of all classes of glycoconjugates, including glycoproteins, polysaccharides, and glycolipids, to the greater scientific community. The Complex Carbohydrate Research Center (CCRC) at the University of Georgia has been home to two NIH/NIGMS federally funded resource centers: the National Center for Biomedical Glycomics (5P41GM103490), where new technologies in mass spectrometry and transcriptome analysis have been developed to investigate glycomics and glycoproteomics of stem and differentiated cell types; and the Research Resource for Integrated Glycotechnology (5P41GM103390), in which mass spectrometry, NMR, molecular modeling, chemical synthesis and cell based assays have been used to investigate the molecular basis of protein-carbohydrate interactions. The CCRC service facility has offered a variety of complementary services using technologies developed by both research resources that are now mature and completely translatable to investigating the glycomes and glycoproteomes of any tissue or cell type. Since the NIGMS Research Resources have recently been sunsetted, we propose to combine the “high demand” services from each resource and create a “National Glycoscience Resource”-CCRC Service and Training (GlyCore) that will enable us to continue to offer and enhance our cutting edge services in glycoscience, hands-on training courses and workshops and to make them easily accessible to the biomedical science community. GlyCore will offer a comprehensive list of services, including isolation and purification, as well as NMR and MS characterization of all glycoconjugates that are grouped into several categories and are integrated together to help solve particular problems in glycoscience. In order to better make our technologies available to outside investigators, we will also continue to offer four well- received and well-established hands-on training courses in glycoscience. Our experience has demonstrated that the one-on-one format of our training is extremely effective in achieving acceptance of tools in glycoscience. We have a combination of state-of-the-art NMR and MS instruments and well-trained scientists with particular expertise and track record for service and training in carbohydrate structural analysis. We have fully implemented and well-developed protocols for sample receipt, sample handling, sample tracking, storage, data archiving, and reporting that provide our users with security and analytic outputs of uniform quality. The number of research groups that have been impacted by our service and training activities spans the whole country and extend globally. We have built an easily accessible “go-to” service and training facility through extensive outreach activities, including our Glyco-booth at meetings, seminars, and workshops at conferences, and building close relationships with universities, scientific non-profit organizations, and industries.