Orbitrap Fusion Tribrid Mass Spectrometer

Orbitrap 融合三联体质谱仪

• 批准号: 8734751
• 负责人: Parastoo Azadi
• 金额: $ 75万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-15 至 2015-07-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8734751
• 关键词: Anthrax disease; Atherosclerosis; Carbohydrates; Cardiac; Communities; Complex; Country; Disease; Educational workshop; Embolism; Funding; Glycosaminoglycans; HIV vaccine; Heart Diseases; Institutes; Laboratories; Lafora Disease; Liquid Chromatography; Lung diseases; Mass Spectrum Analysis; Muscular Dystrophies; Pulmonary Fibrosis; Research; Research Personnel; Research Project Grants; Resolution; Services; System; Training; United States; United States National Institutes of Health; Vaccine Research; Venous; Xenograft procedure; flexibility; instrument; instrumentation; mass spectrometer; nano

DESCRIPTION (provided by applicant): The Analytical Service and Training Laboratory (ASTL) at the Complex Carbohydrate Research Center is requesting funds to purchase a Thermo Scientific Orbitrap Tribrid Fusion mass spectrometer and nano-liquid chromatography system that will be dedicated to glycomics, glycoproteomics and glycosaminoglycan research service projects from a large number of NIH-Investigators from institutes all across the United States. The Orbitrap Fusion at ASTL will have a major impact on many disease related biomedical glycomics projects. This research is significant for muscular dystrophy, HIV vaccine research, heart and lung disease, atherosclerosis and venous thrombo-embolism, cardiac xenotransplantation, Lafora disease, pulmonary fibrosis, anthrax, and many others. The Orbitrap Fusion has unique features such as high sensitivity and resolution, HCD/ETD fragmentation capability, and unprecedented flexibility, which are necessary for all our glycomics and glycoproteomics proposed research projects. Existing LC-MS instrumentation in the facility includes an older LTQ-Orbitrap Discovery, which is not upgradable to ETD/HCD capability and has been discontinued. The number of research groups that will be impacted by the acquisition of the proposed Orbitrap Fusion instrument at the ASTL spans the entire country. Just in the past five years, more than 60 NIH-funded investigators from all over U.S have accessed ASTL's instrumentation and its unique expertise to advance their own carbohydrate research projects. The Orbitrap Fusion will also be utilized in several annual hands-on workshops to train more glycoscientists, and to bring cutting edge mass spectrometry expertise to the larger glycoscience community.

描述（由申请人提供）：复杂碳水化合物研究中心的分析服务和培训实验室（ASTL）正在申请资金购买Thermo Scientific Orbitrap Tribrid Fusion质谱仪和纳米液相色谱系统，该系统将专门用于来自美国各地研究所的大量NIH研究人员的糖组学、糖蛋白组学和糖胺聚糖研究服务项目。ASTL的Orbitrap Fusion将对许多疾病相关的生物医学糖组学项目产生重大影响。这项研究对于肌营养不良症、HIV疫苗研究、心脏和肺部疾病、动脉粥样硬化和静脉血栓栓塞、心脏异种移植、Lafora病、肺纤维化、炭疽病和许多其他疾病具有重要意义。Orbitrap Fusion具有高灵敏度和分辨率、HCD/ETD片段化能力以及前所未有的灵活性等独特功能，这些都是我们所有糖组学和糖蛋白组学研究项目所必需的。该设施中现有的LC-MS仪器包括一个较旧的LTQ-Orbitrap Discovery，该仪器无法与ETD/HCD能力兼容，已停产。在ASTL购买拟议的轨道阱融合仪器将影响到全国各地的研究小组。就在过去的五年里，来自美国各地的60多名NIH资助的研究人员利用ASTL的仪器及其独特的专业知识来推进他们自己的碳水化合物研究项目。Orbitrap Fusion还将用于几个年度实践研讨会，以培训更多的糖科学家，并将尖端的质谱专业知识带到更大的糖科学界。

项目成果

Glycomic and glycoproteomic analysis of glycoproteins-a tutorial.

• DOI: 10.1007/s00216-017-0406-7
• 发表时间: 2017-07
• 期刊: Analytical and bioanalytical chemistry
• 影响因子: 4.3
• 作者: Shajahan A;Heiss C;Ishihara M;Azadi P
• 通讯作者: Azadi P

Generation of 13C-Labeled MUC5AC Mucin Oligosaccharides for Stable Isotope Probing of Host-Associated Microbial Communities.

• DOI: 10.1021/acsinfecdis.8b00296
• 发表时间: 2019-01
• 期刊: ACS infectious diseases
• 影响因子: 5.3
• 作者: Clayton Evert;Tina Loesekann;G. Bhat;A. Shajahan;R. Sonon;P. Azadi;R. Hunter

Vaccines and Therapeutics for COVID-19 - How Can Understanding SARS-CoV-2 Glycosylation Lead to Pharmaceutical Advances?

COVID-19 疫苗和治疗方法 - 了解 SARS-CoV-2 糖基化如何促进制药业进步？

• 发表时间: 2021
• 期刊: American pharmaceutical review
• 作者: Gleinich,AnneS;Pepi,LaurenE;Shajahan,Asif;Heiss,Christian;Azadi,Parastoo
• 通讯作者: Azadi,Parastoo

Properties of the cuticular proteins of Anopheles gambiae as revealed by serial extraction of adults.

• DOI: 10.1371/journal.pone.0175423
• 发表时间: 2017
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Zhou Y;Badgett MJ;Billard L;Bowen JH;Orlando R;Willis JH
• 通讯作者: Willis JH

Glycoproteomic Sample Processing, LC-MS, and Data Analysis Using GlycReSoft.

• DOI: 10.1002/cpz1.84
• 发表时间: 2021-03
• 期刊: Current protocols
• 作者: Wang M;Shajahan A;Pepi LE;Azadi P;Zaia J
• 通讯作者: Zaia J