RACEMIC PROTEIN CRYSTALLOGRAPHY

该子项目是利用资源的许多研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 为子项目列出的总成本可能 代表子项目使用的中心基础设施的估计数量， NCRR赠款不直接向子项目或子项目工作人员提供资金。 采用全化学合成法制备了50个残基的蛇毒蛋白L-奥瓦普林及其对映体D-奥瓦普林。对巨大芽孢杆菌和炭疽芽孢杆菌进行了径向扩散试验; L-和D-奥瓦普林均显示出对B的抗菌活性。巨大的由L-氨基酸组成的天然蛋白质对映体不能容易地结晶。然而，当使用含有等量的L-和D-奥瓦普林的外消旋混合物时，获得衍射质量的晶体。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The 50-residue snake venom protein L-omwaprin and its enantiomer D-omwaprin were prepared by total chemical synthesis. Radial diffusion assays were performed against Bacillus megaterium and Bacillus anthracis; both L- and D-omwaprin showed antibacterial activity against B. megaterium. The native protein enantiomer, made of L-amino acids, failed to crystallize readily. However, when a racemic mixture containing equal amounts of L- and D-omwaprin was used, diffraction quality crystals were obtained.