Research Support Core: Inhalation Toxicology Core

研究支持核心：吸入毒理学核心

• 批准号: 8249996
• 负责人: LARRY W ROBERTSON
• 金额: $ 13.42万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-12 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8249996
• 关键词: Acute; Aerosols; Air; Air Conditioning; Animal Experimentation; Animal Model; Animals; Apical; Area; Autopsy; Binding; Biological; Biological Assay; Blood; Cell Line; Chemicals; Cities; Control Animal; Electronics; Environmental Health; Environmental Wind; Epithelial Cells; Equipment; Experimental Designs; Exposure to; Filtration; Goals; Health; Human; Humidity; Individual; Industry; Inhalation Exposure; Inhalation Toxicology; Laboratories; Laboratory Animals; Life; Lung; Measures; Metabolic; Metabolism; Methods; Molecular Weight; Monitor; Movement; Mus; Neurologic; Nose; Phase; Poison; Pollution; Polychlorinated Biphenyls; Population; Protocols documentation; Rain; Rattus; Relative (related person); Research Personnel; Research Support; Risk Assessment; Rodent; Science; Seasons; Secure; Sentinel; Services; Site; Social Welfare; Soil; Solubility; Source; System; Temperature; Time; Tissues; Toxic Actions; Toxic effect; Toxicology; Water; Work; airway epithelium; alveolar epithelium; animal facility; design; exhaust; health science research; innovation; landfill; novel strategies; operation; particle; planetary Atmosphere; pressure; programs; quality assurance; remediation; reproductive; response; urban area; vapor; vaporization

Inhalation exposure to semi-volatile PCBs in industrial cities, near the Great Lakes, in areas where dredging is occurring and at remediation sites has the potential to cause neurological, reproductive, and immunological toxicity in humans and animals. The Inhalation Toxicology Core was established with the primary goal to serve the projects and other research support cores with state-of-the-art animal facilities and rigorously controlled inhalation exposures to realistic mixtures of airborne PCB congeners. The specific aims of this Core are: Aim 1) Generate controlled PCB atmospheres in exposure chambers and collect airborne PCBs resulting from vaporization of common industrial PCB mixtures (supports Projects 1, 2, 3, 5 and the Analytical and Synthesis Cores); Aim 2) Perform acute, subacute and subchronic inhalation exposures of laboratory animals to defined mixtures of atmospheric PCBs and to individual congeners, (supports Projects 1 and 2); Aim 3) Provide a rodent vivarium for experimental animals used for the isbrp and execute a quality assurance program to monitor their health and welfare (supports Projects 1, 2, and 3); Aim 4) Perform necropies on exposed, control and sentinel rodents and distribute tissues to project investigators in the isbrp (supports Projects 1,2, and 3). The Inhalation Toxicology Core exists within the Pulmonary Toxicology Facility (PTF) of the Environmental Health Sciences Research Center and consists of 6,000 ft2 of contiguous laboratories and offices. The PTF is nationally recognized for innovative animal models and exposure delivery systems. A full array of inhalation toxicology, aerosol science, and bioassay services are rendered. The Inhalation Toxicology Core is well equipped for generating and quantifying PCB vapors or aerosols; for performing nose-only inhalation exposures of rats and mice in acute, subacute or subchronic protocols; and for determining the degree of exposure and response. A new aim in this renewal is: Aim 5) Perform airborne exposures to primary alveolar and airway epithelia and epithelial cell lines in Transwells with an apical air interface using vapor phase and particle bound PCB congeners. This will bring to the program a new approach for studying toxic effects of airborne PCBs on epithelial cells.

在工业城市、五大湖附近、正在进行疏浚的地区和补救地点，吸入接触半挥发性多氯联苯有可能对人类和动物造成神经、生殖和免疫毒性。建立吸入毒理学核心的主要目标是为项目和其他研究支持核心提供最先进的动物设施，并严格控制吸入暴露于空气中多氯联苯同系物的实际混合物。该核心的具体目标是：目标1）在暴露室中产生受控的多氯联苯气氛，并收集常见工业多氯联苯混合物蒸发产生的空气中的多氯联苯（支持项目1、2、3、5以及分析和综合核心）;目标2）对实验室动物进行急性、亚急性和亚慢性吸入暴露于大气中的多氯联苯混合物和个别同系物，（支持项目1和2）;目标3）为用于isbrp的实验动物提供啮齿动物饲养场，并执行质量保证计划以监测其健康和福利（支持项目1、2和3）;目标4）对暴露、对照和哨兵啮齿动物进行尸检，并将组织分发给isbrp的项目研究人员（支持项目1、2和3）。吸入毒理学核心位于环境健康科学研究中心的肺毒理学机构（PTF）内，由6，000平方英尺的连续实验室和办公室组成。PTF因其创新的动物模型和暴露传递系统而获得国家认可。一系列吸入毒理学，气雾剂 提供科学和生物测定服务。吸入毒理学核心设备齐全，可用于生成和量化多氯联苯蒸汽或气溶胶;在急性、亚急性或亚慢性方案中对大鼠和小鼠进行仅鼻吸入暴露;以及确定暴露程度和反应。本次更新的一个新目标是：目标5）使用气相和颗粒结合的多氯联苯同系物，在具有顶端空气界面的Transwell中对初级肺泡和气道上皮细胞和上皮细胞系进行空气暴露。这将为该计划带来一种研究空气中多氯联苯对上皮细胞毒性影响的新方法。