TRANSGENIC SOYBEAN FORMULA TO ENHANCE RESECTION-INDUCED INTESTINAL ADAPTATION

转基因大豆配方可增强切除引起的肠道适应

• 批准号: 8386044
• 负责人: BRAD Wayne WARNER
• 金额: $ 20.28万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-07-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8386044
• 关键词: Address; Ally; Animal Model; Animal Testing; Area; Bacteria; Biological Products; Biotechnology; Catheters; Cessation of life; Coagulation Process; Collaborations; Development; Digestion; Drug Compounding; Drug Formulations; EGFR inhibition; Engineering; Enteral; Enteral Feeding; Enterocytes; Epidermal Growth Factor; Epidermal Growth Factor Receptor; Excision; Foundations; Goals; Human; Infection; Intestines; Intravenous; Kilogram; Laboratories; Length; Liver; Liver Failure; Medical; Milk; Modeling; Morbidity - disease rate; Mus; Nutrient; Operative Surgical Procedures; Oral; Patients; Pediatric Hospitals; Peptides; Pharmacologic Substance; Physicians; Plants; Postoperative Period; Process; Production; Proteins; Research Personnel; Resources; Risk; Rodent Model; Safety; Science; Scientist; Short Bowel Syndrome; Signal Transduction; Small Intestines; Solutions; Soy Milk; Soybeans; Surface; System; Testing; Time; Total Parenteral Nutrition; Toxic effect; Transgenic Organisms; Transplantation; Universities; Veins; Villus; Washington; Weaning; Yeasts; absorption; base; cost; cost effective; experience; forging; improved; medical schools; minimal risk; novel; novel strategies; nutrition; prevent; receptor; research study; response; soy; tube feeding

DESCRIPTION (provided by applicant): Short gut syndrome (SGS) results from the massive intestinal resection that is often necessary in a broad array of medical and surgical treatments. Following a massive small bowel resection (SBR), a compensatory process occurs in the remnant bowel termed adaptation. This response is largely a mitogenic signal culminating in taller villi, deeper crypts, and an expanded mucosal absorptive and digestive surface area. The significance of adaptation is underscored by the requirement in SGS patients of nutrition by vein for the duration of the patient's lifetime if this response is inadequate. It has been demonstrated that exogenous EGF enhances several morphologic parameters of adaptation and its use has been proposed as a treatment for SGS. A unique collaboration between researchers at the Donald Danforth Plant Science Center and physician scientists at the St. Louis Children's Hospital and Washington University School of Medicine will develop and do animal testing of a soy-milk formula produced from soybeans genetically engineered to synthesize EGF. This novel transgenic EGF soy-based formula may provide a low-cost, minimal-risk therapy for SBR patients as a means to reduce SGS. Plant-based production would have many advantages in terms of production costs, world-wide distribution, and stability. This approach may provide a simple means of reducing SGS, and the associated high-cost most often borne by public resources. PUBLIC HEALTH RELEVANCE: Short gut syndrome occurs after a massive small bowel resection (SBR) that is often necessary in a broad array of medical and surgical treatments. After a massive SBR a compensatory process occurs in the remnant bowel termed adaptation which has been shown to be enhanced by Epidermal Growth Factor (EGF). A milk formula produced from soybeans genetically engineered to synthesize EGF will be developed as this may provide a simple, more cost- effective means of enhancing intestinal adaptation in patients suffering from massive intestinal loss.

描述(由申请人提供)：短肠综合征(SGS)是由大量的肠道切除引起的，这在广泛的内科和外科治疗中往往是必要的。在大量小肠切除(SBR)后，残肠发生一个代偿过程，称为适应。这种反应在很大程度上是一种有丝分裂信号，最终导致绒毛更高，隐窝更深，粘膜吸收和消化表面积扩大。在SGS患者中，如果这种反应不充分，则需要在患者一生中通过静脉营养，这突显了适应的重要性。它已经被证明了 外源性EGF增强了适应的几个形态参数，并已被建议用于治疗SGS。唐纳德·丹福斯植物科学中心的研究人员与圣路易斯儿童医院和华盛顿大学医学院的内科科学家开展了一项独特的合作，将开发一种豆奶配方并进行动物试验，这种配方是从转基因大豆中生产出来的，目的是合成EGF。这种新的转基因EGF大豆配方可能为SBR患者提供一种低成本、最低风险的治疗方法，作为减少SGS的一种手段。以工厂为基础的生产在生产成本、全球分布和稳定性方面将有许多优势。这种方法可以提供一种简单的方法来减少SGS，以及通常由公共资源承担的相关高成本。 公共卫生相关性：短肠综合征发生在大规模小肠切除(SBR)后，这在广泛的内科和外科治疗中通常是必要的。在大规模SBR后，残肠发生代偿过程，被称为适应，这已被证明是由表皮生长因子(EGF)促进的。将开发一种由转基因大豆生产的合成EGF的奶粉，因为这可能为患有大量肠道丢失的患者提供一种简单、更具成本效益的增强肠道适应的方法。