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Cooperation and conflict in microbial systems: sucrose metabolism in yeast

微生物系统中的合作与冲突：酵母中的蔗糖代谢

基本信息

批准号：
8257142
负责人：
Jeff Gore
金额：
$ 24.65万
依托单位：
MASSACHUSETTS INSTITUTE OF TECHNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-09-01 至 2014-03-31
项目状态：
已结题

项目摘要

The candidate is starting as an Assistant Professor in the Department of Physics at the Massachusetts Institute of Technology (effective January 2010). The candidate was awarded PhD in Physics from the University of California, Berkeley for research in the field of single-molecule biophysics. During the Postdoctoral Fellowship, the candidate transitioned to the field of systems biology, where he applied the quantitative techniques from previous research to the study of evolutionary game dynamics in microbial systems. The ROO award would allow the candidate to continue to acquire new skills in the techniques of in vivo experimental biology, specifically the use of bacterial and yeast cell culture, flow cytometry, and molecular genetics and analysis. The candidate has developed and engaged in research studying the evolution of cooperation in S. cerevisaie sucrose metabolism. During the Postdoctoral phase of the award, the candidate 1) Developed theoretical and experimental approaches to understand the nature of sucrose metabolism 2) Explored cooperative and competitive interactions in sucrose metabolism and 3) Studied the effect of spatial structure on the evolution of cooperative interactions. These aims were accomplished by genetically engineering "cooperator" and "cheater" strains of S. cerevisiae and growing these strains in direct competition in liquid culture. The results of these competition experiments were monitored through a combination of fluorescence microscopy and flow cytometry. During the independent phase of the award, the candidate will extend the research to the cooperative and competitive interactions that govern the production of iron-scavenging siderophores by the bacteria E. coli.

候选人将在马萨诸塞州的物理系担任助理教授技术学院（2010年1月生效）。该候选人被授予物理学博士学位，加州大学伯克利分校，研究单分子生物物理学。期间博士后奖学金，候选人过渡到系统生物学领域，在那里他应用从以前的研究到微生物进化博弈动力学研究的定量技术系统. ROO奖将允许候选人继续获得新的技能，体内实验生物学，特别是使用细菌和酵母细胞培养，流式细胞术，分子遗传学和分析。候选人已经开发并从事研究，在S.葡萄糖代谢在该奖项的博士后阶段，候选人1）开发理论和实验方法来理解的性质蔗糖代谢2）探索蔗糖代谢中的合作和竞争相互作用; 3）研究了空间结构对合作互动演化的影响。这些目标是通过基因工程改造的“合作者”和“作弊者”的S.酿酒和生长这些菌株在液体培养中直接竞争。这些竞争实验的结果是通过荧光显微术和流式细胞术的组合进行监测。在独立期间在奖励的阶段，候选人将把研究扩展到合作和竞争的互动控制细菌E.杆菌