Quantitation of bioactive molecules using triple quadrupole mass spectrometry

使用三重四极杆质谱法定量生物活性分子

• 批准号: 8247516
• 负责人: Sarah Trotman Pruett
• 金额: $ 41.04万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-04-15 至 2013-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8247516
• 关键词: Address; Aging; Animal Model; Basic Science; Behavioral; Biological; Biological Markers; Biomedical Research; Core Facility; Funding; Goals; Gold; Health; Housing; Human; Laboratories; Mass Spectrum Analysis; Measurement; Measures; Medicine; Mental disorders; Modeling; Molecular; Neurosciences; Neurotransmitters; Obesity; Peptides; Public Health; Research; Research Personnel; Research Support; Sampling; Steroids; System; Systems Analysis; Translational Research; Universities; addiction; base; instrumentation; mass spectrometer; programs; small molecule

DESCRIPTION (provided by applicant): Basic and translation research in behavioral neuroscience are addressing a number of questions that represent a large portion of the of today's largest public health burden imposed by such conditions as addiction, obesity, aging, and mental health disorders. The study of these topics in humans and animal models is crucial for advancing our understanding with the goal of developing new treatment paradigms and medicines. Measurement of small molecules and peptides as regulators of these states is crucial for obtaining this goal. However, valid quantitative strategies are often limited or non-existent for the most relevant models and cross- species comparison of these measures is challenging because measurements are often based on systems that are susceptible to biological matrix interferences. The use of triple quadrupole mass spectrometers is the gold standard for quantitation and provides sensitive and specific molecular measures that are species independent. The Biomarkers Core Laboratory requests funds for the acquisition of a triple quadrupole LC-MS system for the analysis of small molecules such as neurotransmitters, steroids, and peptides. The requested mass spectrometer will support a group of 11 major users at Emory University and analyze an estimated 15,000 biological samples per year. It will be housed in the Biomarkers Core Laboratory which, as an existing core facility, provides research support to a large group of researchers both at Emory and on a national level. The goal for this instrumentation is to provide the ability to access biologically relevant results for Core investigators that would be otherwise unattainable due to sample volume limitations and restrictions inherent to traditional molecular measures. Successful establishment of this instrumentation will enhance multiple research programs at Emory University and nationally, providing the ability to have a direct impact on human health and opening new avenues of biomedical research. PUBLIC HEALTH RELEVANCE: The Biomarkers Core Laboratory at the Yerkes National Primate Research Center functions as a national resource for research support and requests a triple quadrupole mass spectrometer to enhance their support capabilities. The instrumentation will be used for advanced quantitative monitoring of naturally occurring molecules for a broad spectrum of public health challenges including autism, post-traumatic stress, drug addiction, obesity, and genetic diseases.

描述（由申请人提供）：行为神经科学的基础和转化研究正在解决一些问题，这些问题代表了成瘾，肥胖，衰老和精神健康障碍等条件所造成的当今最大公共卫生负担的很大一部分。在人类和动物模型中研究这些主题对于推进我们的理解至关重要，目的是开发新的治疗模式和药物。测量作为这些状态的调节剂的小分子和肽对于实现这一目标至关重要。然而，对于最相关的模型，有效的定量策略通常是有限的或不存在的，并且这些测量的跨物种比较是具有挑战性的，因为测量通常基于易受生物基质干扰的系统。使用三重四极杆质谱仪是定量的金标准，并提供了灵敏和特异的分子测量，是物种独立的。生物标志物核心实验室申请资金用于购买三重四极杆LC-MS系统，用于分析神经递质，类固醇和肽等小分子。所要求的质谱仪将支持埃默里大学的11个主要用户，每年分析约15，000个生物样本。它将被安置在生物标志物核心实验室，作为现有的核心设施，为埃默里大学和国家一级的大量研究人员提供研究支持。该仪器的目标是为核心研究者提供获取生物学相关结果的能力，否则由于样本体积限制和传统分子测量固有的限制，这些结果将无法获得。该仪器的成功建立将加强埃默里大学和全国的多个研究项目，提供对人类健康产生直接影响的能力，并开辟生物医学研究的新途径。 公共卫生相关性：耶基斯国家灵长类动物研究中心的生物标志物核心实验室作为研究支持的国家资源，并要求三重四极质谱仪，以提高其支持能力。该仪器将用于对自然发生的分子进行先进的定量监测，以应对广泛的公共卫生挑战，包括自闭症、创伤后应激障碍、药物成瘾、肥胖和遗传疾病。