Core B - Flow Cytometry Core

流式细胞仪核心(核心B)有三个主要目标。它的主要功能是为中心调查人员提供 能够利用复杂和具有技术挑战性的流式细胞术方法来表征细胞和 它们的功能和信号特性。因此，核心将提供访问最先进的设备，以 流式细胞术分析细胞抗原表达、代谢和信号转导及细胞分选能力 支持路易斯维尔大学在糖尿病和肥胖症方面的心血管研究。核心B级人员 是经验丰富的研究人员，他们在流式细胞术方面工作了几年，使用各种 来自多个物种的组织和细胞，在罕见事件分类和分析方面拥有广泛的专业知识。这个 他们的背景和专长领域的互补性，再加上他们的合议性， 为此核心内部的富有成效的协同及其与其他组织的富有成效的互动奠定坚实基础 与糖尿病和肥胖中心有关的调查人员。其次，核心致力于 教育目标。中心成员不仅将接受仪器操作方面的培训，而且其意图是 所有用户都完全了解细胞学的原理、应用和数据分析。希望所有的项目 在向独立过渡的过程中，研究人员将能够熟练地将流式细胞术作为一种 未来研究中的分析工具。核心还致力于发展其范围和业务，以支持 多维流式细胞术是未来发展的需要。它将继续升级其设备和分析 曲目。最后，这个核心的最终目标是发展成为一个自给自足的大规模流程 不仅满足该中心的具体需求，也满足其他大学的细胞学操作 对糖尿病和肥胖症研究感兴趣的研究人员。一个完全开发的流式细胞仪核心将是一个 在开发未来多调查员项目和帮助研究人员进行 旨在改善糖尿病造成的健康危害的多方面基础和临床研究 还有肥胖症。

The Flow Cytometry Core (Core B) has three major goals. Its primary function is to offer Center Investigators the ability to utilize complex and technically-challenging flow cytometric approaches to characterize cells and their functional and signaling properties. Thus the Core will provide access to state ofthe art equipment for flow cytometric analysis of cellular antigen expression, metabolism and signaling and cell sorting capability to support cardiovascular research in diabetes and obesity at the University of Louisville. Core B personnel are highly-experienced investigators who have worked for several years on flow cytometry using various tissues and cells from multiple species and have extensive expertise in rare event sorting and analysis. The complementary nature of their backgrounds and areas of expertise, coupled with their collegial nature, provides a strong foundation for productive synergy within this Core and its productive interactions with other investigators associated with the Diabetes and Obesity Center. Secondly, the Core is committed to an educational goal. Not only will Center members be trained on the operation of instruments, it is the intent that all users completely understand cytometric theory, application, and data analysis. It is hoped that all Project investigators, in their transition to independence, will be able to competently incorporate flow cytometry as an analytical tool in future studies. The Core is also committed to developing its scope and operation to support multidimensional flow cytometry needs in the future. It will continue to upgrade its equipment and analytical repertoire. Finally this Core has the ultimate goal of developing into a self-supporting and large-scale flow cytometry operation that will cater not only to the specific needs of the Center but to other University investigators interested in diabetes and obesity research. A fully developed Flow Cytometry Core will be a significant asset in developing future multi-investigator projects and for helping researchers conduct multifaceted basic and clinical investigations aimed at the amelioration of health hazards caused by diabetes and obesity.