Core D - Pathology and Flow Cytometry Core

流式细胞术和病理学（核心D）的功能是为每个项目提供专业知识， 流式细胞分析、细胞分选和病理分析所需的设备和设施， 心脏间充质细胞（CMC）和细胞外囊泡（EV）的研究所需的。准确 在复杂异质群体中细胞的鉴定、表征和定量在技术上 要求高，需要专门的和昂贵的设备和人员。的主要优点之一 这个计划项目的一个重要特点是，它将为所有四个项目提供流动的设施和技术， 细胞计数和细胞分选，否则任何项目都无法单独承担。流式细胞仪- 用于鉴定特定细胞群、细胞定型或表型变化的基础分析，以及 细胞功能的表征，如细胞周期，活力或信号转导，将是至关重要的， 项目因此，执行这些技术上具有挑战性的分析的中央公共设施是 为实现项目的目标不可或缺。除了效率和 节省成本，核心还将使四个研究人员与不同的和互补的背景（博士。 O 'Toole、摩尔、Li和Ratajczak）联合收割机结合他们的技术和生物学专业知识，以确保 拟议的工作已成功执行。核心将为计划项目调查人员提供以下能力： 从复杂群体中识别和表征特定细胞类型。它还将提供设施， 进行全面的组织病理学/免疫组织化学分析所需的专业知识， 这个戒酒会这由中心在盲态和严格的 标准化的方式将使在同一项目中比较不同实验的结果成为可能 在不同的项目中。 核心D将是当前资助周期中流式细胞术核心的延续（由O 'Toole博士领导） 以及O 'Toole博士和他的同事们在流式细胞术和病理学方面的密切和富有成效的持续合作。 和其他PPG调查员核心D的职能是为所有四个项目提供专门知识， 流式细胞术、分选和病理学所需的设备、设施、试剂、程序和分析 工作具体而言，核心将i）进行流式细胞术分析和细胞分选程序， CMC和EV的研究，ii）进行组织处理、组织病理学和心脏组织的免疫组织化学， 样本，以及iii）向四个项目提供核心的丰富经验和专业知识 人员的

The function of the Flow Cytometry and Pathology (Core D) is to provide each Project with the expertise, equipment, and facilities required for the flow cytometric analyses, cell sorting, and pathological analyses that are needed for studies of cardiac mesenchymal cells (CMCs) and extracellular vesicles (EVs). Accurate identification, characterization, and quantitation of cells in a complex heterogeneous population are technically demanding and require specialized and expensive equipment and personnel. One of the major advantages of this Program Project is that it will make available to all four Projects the facilities and techniques of flow cytometry and cell sorting, which otherwise would not be affordable to any Project in isolation. Flow cytometry- based analysis for the identification of specific cell populations, changes in cell commitment or phenotype, and characterization of cell functions such as cell cycle, viability, or signal transduction, will be crucial to all Projects. A central common facility that performs these technically challenging analyses is therefore indispensable to achieve the goals of the Program Project. Besides the obvious advantages of efficiency and cost savings, the Core will also enable four investigators with diverse and complementary backgrounds (Drs. O’Toole, Moore, Li, and Ratajczak) to combine their technical and biological expertise in order to ensure that the proposed work is successfully executed. The Core will offer the Program Project investigators the ability to identify and characterize specific cell types from complex populations. It will also offer the facilities and expertise necessary to perform the comprehensive histopathologic/immunohistochemical analyses required for this Program. The uniform histopathologic analyses conducted by the Core in a blinded and rigorously standardized fashion will make it possible to compare results of different experiments within the same Project and among different Projects. Core D will be the continuation of the Flow Cytometry Core in the current funding cycle (led by Dr. O’Toole) and of the intense and productive ongoing collaboration in flow cytometry and pathology between Dr. O’Toole and the other PPG investigators. The function of Core D is to provide all four Projects with the expertise, equipment, facilities, reagents, procedures, and analyses needed for flow cytometry, sorting, and pathology work. Specifically, the Core will i) perform the flow cytometric analyses and cell sorting procedures required for studies of CMCs and EVs, ii) perform tissue processing, histopathology, and immunohistochemistry of cardiac samples, and iii) make available to the four Projects the tremendous experience and expertise of the Core personnel.