LTQ Velos Pro mass spectrometer with ETD and other options

描述(由申请人提供)： 这项提案寻求从ThermoFisher购买LTQ Velos Pro质谱仪的资金，该质谱仪具有ETD选项和NanoLC。该仪器提供的快速循环时间、高灵敏度和多种离子碎裂模式的组合对于这项提议的主要用户群体是不可用的，但将允许在定量蛋白质组和定量磷蛋白质组基础上的质谱学实验的基本增强，以支持加州大学国立卫生研究院资助的一些研究项目。将得到加强的具体项目包括研究牛痘病毒感染时细胞信号调节的分子机制(Gershon博士)；对脊髓灰质炎和鼻病毒以及衣原体感染的全球细胞反应(塞姆勒博士)和衣原体感染(德拉马扎博士)，了解蚊子媒介对传染病的唾液腺和中肠反应(詹姆斯博士)，早期检测尿液蛋白质组中的前列腺癌标记物(梅科拉博士)，由于Pim-1激酶过度表达和特异性抑制而导致的亚磷酸盐占有率的全球变化(礼来博士)，寻找与血管分支有关的生长因子(休斯博士)，在人类mRNA剪接复合体的组装过程中发生的修改的可塑性(赫特尔博士)，了解大脑中蛋白质老化的反应，以及关键调控酶一氧化氮合酶的分子动力学(Poulos博士)。这项提案的私家侦探拥有12年的蛋白质质谱学经验，将在前述合作者的支持下，在一个监督委员会的监督下管理仪器，监督委员会的外部成员包括加州大学洛杉矶分校的Joe Loo博士和Julian Whitelege博士，以及代表基础和临床项目的内部委员会成员。美国国立卫生研究院支持的21个与人类健康有关的研究项目，涉及UCI两所学校的六个系，预计将从拟议工具的能力中受益匪浅。所要求的仪器将是UCI医学院和生物科学学院内跨学科生物医学研究能力的重大进步。

DESCRIPTION (provided by applicant): This proposal seeks funding for the purchase of an LTQ Velos Pro mass spectrometer from ThermoFisher, with ETD option and nanoLC. The combination of fast cycle times, high sensitivity and multiple ion fragmentation modes provided by this instrument is not available to the major user group for this proposal, yet would allow fundamental enhancements in quantitative proteome- and quantitative phosphoproteome-based mass spectrometry experiments in support of a number of NIH funded research programs at UCI. Specific projects that will be enhanced include investigation of the molecular mechanisms underlying the modulation of cell signaling upon infection by vaccinia virus (Dr. Gershon); global cellular responses to polio and rhinovirus (Dr. Semler) and Chlamydia infection (Dr. DeLa Maza), understanding the salivary gland and midgut responses of mosquito vectors to infectious diseases (Dr. James), early detection of prostate cancer markers in the urinary proteome (Dr. Mercola), global changes in phosphosite occupancy as a result of Pim-1 kinase over-expression and specific inhibition (Dr. Lilly), finding growth factors involved in blood vessel branching durig embryogenesis and their homeostasis (Dr. Hughes), plasticity of modifications incurred during the assembly of human mRNA splicing complexes (Dr. Hertel), understanding responses to protein aging in the brain, and molecular dynamics of the key regulatory enzyme nitric oxide synthase (Dr. Poulos). The P.I. of this proposal, with 12 years experience in protein mass spectrometry, will administer the instrument in a shared area with the blessing of the aforementioned collaborators under the oversight of a supervisory committee with external members including Dr. Joe Loo (UCLA) and Dr. Julian Whitelegge (UCLA), and internal committee members representing both basic and clinical programs. 21 NIH-supported research programs related to human health spanning six departments in two schools of UCI are expected to benefit significantly from the capabilities of the proposed instrument. The requested instrument would represent a significant advance in interdisciplinary biomedical research capabilities within the UCI Schools of Medicine and Biological Sciences.