LTQ Velos Pro mass spectrometer with ETD and other options

具有 ETD 和其他选件的 LTQ Velos Pro 质谱仪

• 批准号: 8447950
• 负责人: Paul D Gershon
• 金额: $ 37.41万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8447950
• 关键词: Aging; Area; Biological Sciences; Biomedical Research; Blood Vessels; Brain; Chlamydia Infections; Clinical; Committee Members; Communicable Diseases; Complex; Embryonic Development; Enzymes; Funding; Global Change; Growth Factor; Health; Homeostasis; Human; Infection; Investigation; Ions; Mass Spectrum Analysis; Messenger RNA; Midgut; Modification; Molecular; N.I.H. Research Support; Nitric Oxide Synthase; Poliomyelitis; Proteins; Proteome; RNA Splicing; Research; Rhinovirus; Salivary Glands; Schools; Screening for Prostate Cancer; Signal Transduction; Time; United States National Institutes of Health; Vaccinia virus; base; experience; instrument; mass spectrometer; medical schools; member; molecular dynamics; programs; proto-oncogene protein pim-1; research study; response; urinary; vector mosquito

DESCRIPTION (provided by applicant): This proposal seeks funding for the purchase of an LTQ Velos Pro mass spectrometer from ThermoFisher, with ETD option and nanoLC. The combination of fast cycle times, high sensitivity and multiple ion fragmentation modes provided by this instrument is not available to the major user group for this proposal, yet would allow fundamental enhancements in quantitative proteome- and quantitative phosphoproteome-based mass spectrometry experiments in support of a number of NIH funded research programs at UCI. Specific projects that will be enhanced include investigation of the molecular mechanisms underlying the modulation of cell signaling upon infection by vaccinia virus (Dr. Gershon); global cellular responses to polio and rhinovirus (Dr. Semler) and Chlamydia infection (Dr. DeLa Maza), understanding the salivary gland and midgut responses of mosquito vectors to infectious diseases (Dr. James), early detection of prostate cancer markers in the urinary proteome (Dr. Mercola), global changes in phosphosite occupancy as a result of Pim-1 kinase over-expression and specific inhibition (Dr. Lilly), finding growth factors involved in blood vessel branching durig embryogenesis and their homeostasis (Dr. Hughes), plasticity of modifications incurred during the assembly of human mRNA splicing complexes (Dr. Hertel), understanding responses to protein aging in the brain, and molecular dynamics of the key regulatory enzyme nitric oxide synthase (Dr. Poulos). The P.I. of this proposal, with 12 years experience in protein mass spectrometry, will administer the instrument in a shared area with the blessing of the aforementioned collaborators under the oversight of a supervisory committee with external members including Dr. Joe Loo (UCLA) and Dr. Julian Whitelegge (UCLA), and internal committee members representing both basic and clinical programs. 21 NIH-supported research programs related to human health spanning six departments in two schools of UCI are expected to benefit significantly from the capabilities of the proposed instrument. The requested instrument would represent a significant advance in interdisciplinary biomedical research capabilities within the UCI Schools of Medicine and Biological Sciences.

描述（由申请人提供）： 该提案寻求资金从 ThermoFisher 购买具有 ETD 选项和 nanoLC 的 LTQ Velos Pro 质谱仪。该仪器提供的快速循环时间、高灵敏度和多离子碎裂模式的组合不适用于本提案的主要用户组，但可以从根本上增强定量蛋白质组和定量磷酸蛋白质组质谱实验，以支持 NIH 资助的 UCI 的许多研究项目。将加强的具体项目包括研究痘苗病毒感染后细胞信号传导调节的分子机制（Gershon 博士）；对脊髓灰质炎和鼻病毒（Semler 博士）和衣原体感染（DeLa Maza 博士）的整体细胞反应，了解蚊媒对传染病的唾液腺和中肠反应（James 博士），尿蛋白质组中前列腺癌标志物的早期检测（Mercola 博士），Pim-1 激酶过度表达和特异性导致的磷酸位点占据的整体变化 抑制（Lilly 博士），寻找胚胎发生过程中血管分支及其稳态所涉及的生长因子（Hughes 博士），人类 mRNA 剪接复合物组装过程中发生的修饰的可塑性（Hertel 博士），了解大脑中蛋白质老化的反应，以及关键调节酶一氧化氮合酶的分子动力学（Poulos 博士）。 P.I.该提案的负责人拥有 12 年蛋白质质谱分析经验，将在上述合作者的支持下，在监督委员会的监督下，在共享区域管理该仪器，该监督委员会的外部成员包括 Joe Loo 博士（加州大学洛杉矶分校）和 Julian Whitelegge 博士（加州大学洛杉矶分校），以及代表基础和临床项目的内部委员会成员。 21 个由 NIH 支持的、涉及 UCI 两所学校六个系的人类健康研究项目预计将从拟议仪器的功能中受益匪浅。所要求的仪器将代表 UCI 医学和生物科学学院跨学科生物医学研究能力的重大进步。