LTQ Velos Pro mass spectrometer with ETD and other options

描述（由申请人提供）： 该提案寻求资助从ThermoFisher购买LTQ Velos Pro质谱仪，带有ETD选项和nanoLC。该仪器提供的快速循环时间，高灵敏度和多离子碎片模式的组合不适用于该提案的主要用户组，但将允许定量蛋白质组和定量磷酸化蛋白质组的质谱实验的基本增强，以支持UCI的一些NIH资助的研究计划。将加强的具体项目包括研究牛痘病毒感染后细胞信号调节的分子机制（格申博士）;全球细胞对脊髓灰质炎和鼻病毒的反应（塞姆勒博士）和衣原体感染（德拉马扎博士），了解蚊子媒介对传染病的唾液腺和中肠反应（詹姆斯博士），早期检测前列腺癌标志物在尿蛋白质组（Mercola博士），Pim-1激酶过表达和特异性抑制导致磷酸化位点占据率的总体变化发现生长因子在胚胎发育过程中参与血管分支及其体内平衡（休斯博士），在人类mRNA剪接复合体组装过程中发生的修饰的可塑性（Hertel博士），了解大脑中蛋白质老化的反应，以及关键调节酶一氧化氮合酶的分子动力学（Poulos博士）。私家侦探该提案的一部分，具有12年的蛋白质质谱分析经验，将在上述合作者的祝福下，在监督委员会的监督下，在一个共享区域管理该仪器，外部成员包括Joe Loo博士（UCLA）和Julian Whitelegge博士（UCLA），以及代表基础和临床项目的内部委员会成员。21个NIH支持的与人类健康相关的研究项目，横跨UCI两个学院的六个部门，预计将从拟议仪器的能力中受益匪浅。所要求的工具将代表UCI医学和生物科学学院跨学科生物医学研究能力的重大进步。

DESCRIPTION (provided by applicant): This proposal seeks funding for the purchase of an LTQ Velos Pro mass spectrometer from ThermoFisher, with ETD option and nanoLC. The combination of fast cycle times, high sensitivity and multiple ion fragmentation modes provided by this instrument is not available to the major user group for this proposal, yet would allow fundamental enhancements in quantitative proteome- and quantitative phosphoproteome-based mass spectrometry experiments in support of a number of NIH funded research programs at UCI. Specific projects that will be enhanced include investigation of the molecular mechanisms underlying the modulation of cell signaling upon infection by vaccinia virus (Dr. Gershon); global cellular responses to polio and rhinovirus (Dr. Semler) and Chlamydia infection (Dr. DeLa Maza), understanding the salivary gland and midgut responses of mosquito vectors to infectious diseases (Dr. James), early detection of prostate cancer markers in the urinary proteome (Dr. Mercola), global changes in phosphosite occupancy as a result of Pim-1 kinase over-expression and specific inhibition (Dr. Lilly), finding growth factors involved in blood vessel branching durig embryogenesis and their homeostasis (Dr. Hughes), plasticity of modifications incurred during the assembly of human mRNA splicing complexes (Dr. Hertel), understanding responses to protein aging in the brain, and molecular dynamics of the key regulatory enzyme nitric oxide synthase (Dr. Poulos). The P.I. of this proposal, with 12 years experience in protein mass spectrometry, will administer the instrument in a shared area with the blessing of the aforementioned collaborators under the oversight of a supervisory committee with external members including Dr. Joe Loo (UCLA) and Dr. Julian Whitelegge (UCLA), and internal committee members representing both basic and clinical programs. 21 NIH-supported research programs related to human health spanning six departments in two schools of UCI are expected to benefit significantly from the capabilities of the proposed instrument. The requested instrument would represent a significant advance in interdisciplinary biomedical research capabilities within the UCI Schools of Medicine and Biological Sciences.