LTQ Velos Pro mass spectrometer with ETD and other options
LTQ Velos Pro mass spectrometer with ETD and other options
批准号:
8447950
负责人:
Paul D Gershon
金额:
$37.41万
依托单位国家:
美国
项目类别:
财政年份:
2013
资助国家:
美国
项目状态:
已结题
起止时间:
2013-07-01 至 2014-06-30
关键词:
AgingAreaBiological SciencesBiomedical ResearchBlood VesselsBrainChlamydia InfectionsClinicalCommittee MembersCommunicable DiseasesComplexEmbryonic DevelopmentEnzymesFundingGlobal ChangeGrowth FactorHealthHomeostasisHumanInfectionInvestigationIonsMass Spectrum AnalysisMessenger RNAMidgutModificationMolecularN.I.H. Research SupportNitric Oxide SynthasePoliomyelitisProteinsProteomeRNA SplicingResearchRhinovirusSalivary GlandsSchoolsScreening for Prostate CancerSignal TransductionTimeUnited States National Institutes of HealthVaccinia virusbaseexperienceinstrumentmass spectrometermedical schoolsmembermolecular dynamicsprogramsproto-oncogene protein pim-1research studyresponseurinaryvector mosquito
中文摘要
描述(由申请人提供):
英文摘要
DESCRIPTION (provided by applicant):
This proposal seeks funding for the purchase of an LTQ Velos Pro mass spectrometer from ThermoFisher, with ETD option and nanoLC. The combination of fast cycle times, high sensitivity and multiple ion fragmentation modes provided by this instrument is not available to the major user group for this proposal, yet would allow fundamental enhancements in quantitative proteome- and quantitative phosphoproteome-based mass spectrometry experiments in support of a number of NIH funded research programs at UCI. Specific projects that will be enhanced include investigation of the molecular mechanisms underlying the modulation of cell signaling upon infection by vaccinia virus (Dr. Gershon); global cellular responses to polio and rhinovirus (Dr. Semler) and Chlamydia infection (Dr. DeLa Maza), understanding the salivary gland and midgut responses of mosquito vectors to infectious diseases (Dr. James), early detection of prostate cancer markers in the urinary proteome (Dr. Mercola), global changes in phosphosite occupancy as a result of Pim-1 kinase over-expression and specific inhibition (Dr. Lilly), finding growth factors involved in blood vessel branching durig embryogenesis and their homeostasis (Dr. Hughes), plasticity of modifications incurred during the assembly of human mRNA splicing complexes (Dr. Hertel), understanding responses to protein aging in the brain, and molecular dynamics of the key regulatory enzyme nitric oxide synthase (Dr. Poulos). The P.I. of this proposal, with 12 years experience in protein mass spectrometry, will administer the instrument in a shared area with the blessing of the aforementioned collaborators under the oversight of a supervisory committee with external members including Dr. Joe Loo (UCLA) and Dr. Julian Whitelegge (UCLA), and internal committee members representing both basic and clinical programs. 21 NIH-supported research programs related to human health spanning six departments in two schools of UCI are expected to benefit significantly from the capabilities of the proposed instrument. The requested instrument would represent a significant advance in interdisciplinary biomedical research capabilities within the UCI Schools of Medicine and Biological Sciences.
期刊论文(2)
专著(0)
科研奖励(0)
会议论文
Nuclear functions co-opted by human rhinovirus during replication in the cytoplasm of infected cells
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批准号:10684733
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项目类别:
-
资助金额:$46.57万
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财政年份:2021
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负责人:Paul D Gershon
-
依托单位:
Nuclear functions co-opted by human rhinovirus during replication in the cytoplasm of infected cells
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批准号:10443844
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项目类别:
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资助金额:$46.57万
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财政年份:2021
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负责人:Paul D Gershon
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依托单位:
Nuclear functions co-opted by human rhinovirus during replication in the cytoplasm of infected cells
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批准号:10298555
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项目类别:
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资助金额:$46.25万
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财政年份:2021
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负责人:Paul D Gershon
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依托单位:
Molecular architecture of the Vaccinia virion by structural proteomics
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批准号:10179428
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项目类别:
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资助金额:$33.2万
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财政年份:2019
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负责人:Paul D Gershon
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依托单位:
Molecular architecture of the Vaccinia virion by structural proteomics
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批准号:10465049
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项目类别:
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资助金额:$33.07万
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财政年份:2019
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负责人:Paul D Gershon
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依托单位:
Molecular architecture of the Vaccinia virion by structural proteomics
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批准号:10022126
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项目类别:
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资助金额:$33.32万
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财政年份:2019
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负责人:Paul D Gershon
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依托单位:
Novel nuclear and intracellular pathology in early AD
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批准号:8702666
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项目类别:
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资助金额:$24.45万
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财政年份:2014
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负责人:Paul D Gershon
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依托单位:
The Pox Virion Molecular Interactome
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批准号:8582931
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项目类别:
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资助金额:$21.73万
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财政年份:2013
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负责人:Paul D Gershon
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依托单位:
The Pox Virion Molecular Interactome
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批准号:8731174
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项目类别:
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资助金额:$19.31万
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财政年份:2013
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负责人:Paul D Gershon
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依托单位:
PROTEIN MASS SPECTROMETRY (SHARED RESOURCE)
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批准号:7944552
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项目类别:
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资助金额:$2.14万
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财政年份:2009
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负责人:Paul D Gershon
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依托单位:
MALDI TOF MASS SPECTROMETER
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批准号:6292199
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项目类别:
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资助金额:$30.5万
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财政年份:2001
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负责人:Paul D Gershon
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依托单位:
PROTEIN MASS SPECTROMETRY (SHARED RESOURCE)
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批准号:8740838
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项目类别:
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资助金额:$4.76万
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财政年份:1997
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负责人:Paul D Gershon
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依托单位:
Mechanism of Poly(a) Tail Formation by Vaccinia Virus
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批准号:6928609
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项目类别:
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资助金额:$27.45万
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财政年份:1995
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负责人:Paul D Gershon
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依托单位:
Mechanism of Poly(a) Tail Formation by Vaccinia Virus
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批准号:7267766
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项目类别:
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资助金额:$26.03万
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财政年份:1995
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负责人:Paul D Gershon
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依托单位:
MECHANISM OF POLY(A) TAIL FORMATION BY VACCINIA VIRUS
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批准号:2190762
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项目类别:
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资助金额:$16.28万
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财政年份:1995
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负责人:Paul D Gershon
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依托单位:
MECHANISM OF POLYA TAIL FORMATION BY VACCINIA VIRUS
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批准号:2908994
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项目类别:
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资助金额:$24.76万
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财政年份:1995
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负责人:Paul D Gershon
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依托单位:
MECHANISM OF POLYA TAIL FORMATION BY VACCINIA VIRUS
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批准号:6331989
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项目类别:
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资助金额:$23.43万
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财政年份:1995
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负责人:Paul D Gershon
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依托单位:
MECHANISM OF POLY(A) TAIL FORMATION BY VACCINIA VIRUS
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批准号:2459599
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项目类别:
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资助金额:$16.92万
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财政年份:1995
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负责人:Paul D Gershon
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依托单位:
MECHANISM OF POLYA TAIL FORMATION BY VACCINIA VIRUS
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批准号:6496117
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项目类别:
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资助金额:$1.5万
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财政年份:1995
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负责人:Paul D Gershon
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依托单位:
Mechanism of Poly(a) Tail Formation by Vaccinia Virus
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批准号:6823030
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项目类别:
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资助金额:$27.3万
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财政年份:1995
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负责人:Paul D Gershon
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依托单位:
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