PROTEIN MASS SPECTROMETRY (SHARED RESOURCE)

蛋白质鉴定和蛋白质组学是癌症研究的重要新工具。蛋白质质谱共享资源将提供生物分子和蛋白质分析的高水平质谱。该共享资源基于一对串联质量分析仪（AB 4700 MALDITOF/ TOF和Thermo LTQ nanoESI-QIT），可访问多达10个HPLC系统，其中包含10个四元泵和2个二元泵，7台运行至少21个软件包的PC以及许多较小的项目。该设施能够从聚丙烯酰胺凝胶切片中常规鉴定蛋白质。此外，它还可以常规鉴定下拉混合物、复杂病毒体和全细胞提取物中的蛋白质。他们在LC-MALDI分析之前进行了多维（最多三维）串联HPLC。这种能力包括二维蛋白质分级分离（PF 2D）和二维和三维串联肽分级分离。该设施为最先进的蛋白质组学前沿提供了一个令人兴奋的跳板。 在过去的三年里，蛋白质质谱仪设施一直得到癌症中心的支持，作为一个发展中的共享资源。现在要求永久资金作为全面共享资源提供支持。

Protein identification and proteomics are important new tools in cancer research. The Protein Mass Spectrometry Shared Resource will provide high-level mass spectrometry of biomolecules and protein analyses. This Shared Resource is based around a pair of tandem mass analyzers (the AB 4700 MALDITOF/ TOF and Thermo LTQ nanoESI-QIT), with access to as many as ten HPLC systems containing 10 quaternary and two binary pumps, seven PCs running at least 21 software packages, and numerous smaller items. The Facility is able to routinely identify proteins in slices from polyacryamide gels. In addition, it can routinely identify proteins in pull-down mixtures, complex virions, and whole cell extracts. They have done multi-dimensional (up to three dimensions) of tandem HPLC prior to LC-MALDI analysis. This capability includes two-dimensional protein fractionation (PF2D) and two and three dimensions of tandem peptide fractionation. This Facility provides an exciting springboard to the frontiers of state-of-the art proteomics. The Protein Mass Spectrometry Facility has been supported by the Cancer Center as a developing Shared Resource for the past three years. Permanent funds are now requested for support as a full Shared Resource.