PROTEIN MASS SPECTROMETRY (SHARED RESOURCE)

蛋白质鉴定和蛋白质组学是癌症研究的重要新工具。蛋白质质量 光谱共享资源将提供生物分子和蛋白质的高水平质谱学 分析。此共享资源基于一对串联质谱仪(AB 4700 MALDITOF/ TOF和Thermo LTQ Nano ESI-QIT)，可以访问多达10个包含10个 四元泵和两个二元泵，七台运行至少21个软件包的PC，以及许多较小的 物品。该设备能够常规地识别聚丙烯酰胺凝胶切片中的蛋白质。此外，它还可以 常规鉴定下拉混合物、复合病毒粒子和全细胞提取物中的蛋白质。他们已经做了 在LC-MALDI分析之前进行多维(最多三维)串联高效液相色谱分析。这一能力 包括二维蛋白质分级(PF2D)和二维和三维串联多肽 分馏法。这一设施提供了一个令人兴奋的跳板，通向最先进的蛋白质组学的前沿。 蛋白质质谱学设备已经得到癌症中心的支持，作为一个正在开发的共享 过去三年的Fesource。现在需要永久资金作为完全共享的支持 资源。

Protein identification and proteomics are important new tools in cancer research. The Protein Mass Spectrometry Shared Resource will provide high-level mass spectrometry of biomolecules and protein analyses. This Shared Resource is based around a pair of tandem mass analyzers (the AB 4700 MALDITOF/ TOF and Thermo LTQ nanoESI-QIT), with access to as many as ten HPLC systems containing 10 quaternary and two binary pumps, seven PCs running at least 21 software packages, and numerous smaller items. The Facility is able to routinely identify proteins in slices from polyacryamide gels. In addition, it can routinely identify proteins in pull-down mixtures, complex virions, and whole cell extracts. They have done multi-dimensional (up to three dimensions) of tandem HPLC prior to LC-MALDI analysis. This capability includes two-dimensional protein fractionation (PF2D) and two and three dimensions of tandem peptide fractionation. This Facility provides an exciting springboard to the frontiers of state-of-the art proteomics. The Protein Mass Spectrometry Facility has been supported by the Cancer Center as a developing Shared Fesource for the past three years. Permanent funds are now requested for support as a full Shared Resource.