Diagnosis of Osteochondrosis using high field MRI

使用高场 MRI 诊断骨软骨病

• 批准号: 8490466
• 负责人: Cathy S. Carlson
• 金额: $ 14.13万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-07-01 至 2015-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8490466
• 关键词: 3-Dimensional; Adolescent; Affect; Animal Model; Animals; Area; Barium Sulfate; Biology; Biomechanics; Blood Vessels; Calcified; Cartilage; Cartilage Matrix; Child; Childhood; Chronic; Clinical; Collagen; Collagen Type I; Computer Simulation; Degenerative polyarthritis; Development; Diagnosis; Diagnostic Procedure; Disease; Distal; Early Diagnosis; Equus caballus; Excision; Failure; Family suidae; Femur; Fibrocartilages; Frostbite; Functional disorder; Funding; Goals; Growth; Healed; Human; Hyaline Cartilage; Image; Imaging Techniques; Imaging technology; Immunohistochemistry; Injury; Joints; K-Series Research Career Programs; Knowledge; Lesion; Life Style; Magnetic Resonance; Magnetic Resonance Imaging; Magnetism; Maps; Mentors; Methodology; Methods; Minnesota; Monitor; Necrosis; Operative Surgical Procedures; Orthopedic Surgery procedures; Orthopedics; Osteochondritis Dissecans; Osteochondrosis; Pain; Pathogenesis; Pathologist; Physiologic pulse; Prevalence; Proteoglycan; Qualifying; Recommendation; Replacement Arthroplasty; Request for Applications; Research; Research Activity; Research Personnel; Role; Scientist; Secondary to; Sepsis; Services; Signal Transduction; Sirius Red F3B; Site; Specimen; Staging; Staining method; Stains; Surgical Flaps; Techniques; Time; Tissues; Tolonium chloride; Toxin; Trauma; Universities; Vascular blood supply; Veterinary Medicine; Wages; Water; Work; abstracting; aggrecan; arthropathies; bone; career; cartilage repair; college; disability; experience; healing; humerus; imaging modality; improved; in vivo; innovation; joint injury; knee pain; member; method development; novel; prevent; professor; repaired; technique development; young adult

DESCRIPTION (provided by applicant): Project Summary/Abstract Dr. Cathy Carlson is an experienced, ACVP-boarded veterinary pathologist and orthopaedic researcher who is a tenured full professor in the College of Veterinary Medicine at the University of Minnesota. Although Dr. Carlson is an active research collaborator, gradually increasing service commitments and other responsibilities have prevented her from having a more central role in research in recent years. In this application, she requests 50% salary support for two years while she acquires new knowledge in the area of high field magnetic resonance imaging (MRI) and applies this knowledge to the development of methods to diagnose subclinical lesions of osteochondrosis. For these studies, she will use a well-characterized animal model of osteochondrosis (spontaneous disease in young pigs) with which she has considerable expertise. She will be assisted in these studies by her primary mentor, Dr. Michael Garwood, who is an expert in the area of high field MRI technique development, and her mentoring committee: Dr. Jutta Ellermann (expert in human orthopaedic MRI, including osteochondrosis), Dr. Denis Clohisy (chair of the Department of Orthopaedic Surgery and experienced orthopaedic researcher), and Dr. Jack Lewis (cartilage matrix biology and biomechanics expert). In the proposed, highly innovative studies, the extensive knowledge and experience that Dr. Carlson has with a well-documented animal model of osteochondrosis will be combined with the state-of- the-art high field MRI expertise of the mentor group to identify subclinical lesions of osteochondrosis in intact joints of young pigs, in which the disease occurs with nearly 100% prevalence. Newly developed high field MRI techniques will be used to detect and characterize early damage to the cartilage. The specific aims of the proposed studies are to 1) Develop high-field MRI techniques to identify and characterize subclinical lesions of osteochondrosis, (locally extensive areas of cartilage necrosis), in intact and disarticulated specimens of distal humerus and distal femur (most common predilection sites in both swine and humans) from young pigs; 2) Develop quantitative techniques to characterize the collagen and proteoglycan components of the lesions for comparison with the MRI results; and 3) Develop high-field MRI techniques to identify and map cartilage canal blood vessels and validate these in cleared specimens in which the vessels have been perfused with barium sulfate. This career development award will allow Dr. Carlson the dedicated time to 1) gain expertise in MRI methodology; 2) interact closely with the scientists at the Center for Magnetic Imaging Research; 3) identify and acquire the appropriate porcine specimens for study; 4) assist in interpreting the MRI findings and modifying the techniques through parallel histological studies in the same sites as those evaluated by MRI; and 5) interact with a highly qualified mentor group, the members of which will provide both specific and global input to her proposed research. Dr. Carlson's long-term career goal is to remain in academic research, obtain and maintain RO1 funding for this project, and to devote at least 75% of her effort to research activities.

描述（由申请人提供）：项目摘要/摘要Cathy Carlson博士是一位经验丰富的ACVP兽医病理学家和骨科研究员，是明尼苏达大学兽医学院的终身教授。虽然卡尔森博士是一个积极的研究合作者，但近年来逐渐增加的服务承诺和其他责任使她无法在研究中发挥更重要的作用。在这份申请中，她要求获得两年50%的工资支持，同时获得高场磁共振成像（MRI）领域的新知识，并将这些知识应用于诊断骨软骨病亚临床病变的方法开发。在这些研究中，她将使用一种特征良好的骨软骨病（幼猪自发性疾病）动物模型，她在这方面具有相当的专业知识。她将在这些研究中得到她的主要导师Michael Garwood博士及其指导委员会的协助，Michael Garwood博士是高场MRI技术开发领域的专家：Jutta Ellermann博士（人类骨科MRI专家，包括骨软骨病），Denis Clohisy博士（骨科主任，经验丰富的骨科研究员），和杰克刘易斯博士（软骨基质生物学和生物力学专家）。在拟议的高度创新的研究中，Carlson博士在骨软骨病动物模型方面的广泛知识和经验将与导师小组最先进的高场MRI专业知识相结合，以确定幼猪完整关节中骨软骨病的亚临床病变，其中该疾病的发生率接近100%。新开发的高场MRI技术将用于检测和表征软骨的早期损伤。拟议研究的具体目的是：1）开发高场MRI技术，以识别和表征骨软骨病的亚临床病变，（局部广泛的软骨坏死区域），在肱骨远端和股骨远端的完整和离断标本中（猪和人中最常见的好发部位）; 2）开发定量技术来表征病变的胶原和蛋白多糖成分，以与MRI结果进行比较;和3）开发高场MRI技术以识别和映射软骨管血管，并在已用硫酸钡灌注血管的透明标本中验证这些。该职业发展奖将使Carlson博士有专门的时间1）获得MRI方法学方面的专业知识; 2）与磁成像研究中心的科学家密切互动; 3）识别并获得适当的猪标本进行研究; 4）通过在MRI评估的相同部位进行平行组织学研究，协助解释MRI结果并修改技术;和5）与高素质的导师小组互动，该小组的成员将为她提出的研究提供具体和全球的投入。卡尔森博士的长期职业目标是继续从事学术研究，获得并维持该项目的RO 1资金，并将至少75%的精力投入研究活动。